Construct: ORF TRCN0000468550

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004834.1_s317c1
Derived from:
ccsbBroadEn_13628
DNA Barcode:
ACATGGAGTCAGTTCTTTTTGGCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WASH3P (374666)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468550
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 100287171 WASHC1 WASH complex subunit 1 NM_182905.5 97.8% 96.3% (many diffs)
2 human 100287171 WASHC1 WASH complex subunit 1 XM_024447369.1 97.8% 96.3% (many diffs)
3 human 100287171 WASHC1 WASH complex subunit 1 XM_011517662.3 96.6% 85% (many diffs)
4 human 100287171 WASHC1 WASH complex subunit 1 XM_011517658.2 95% 93.5% (many diffs)
5 human 100287171 WASHC1 WASH complex subunit 1 XM_011517659.2 94.6% 83.5% (many diffs)
6 human 100287171 WASHC1 WASH complex subunit 1 XM_011517660.2 94.5% 84.1% (many diffs)
7 human 100287171 WASHC1 WASH complex subunit 1 XM_011517665.2 90.9% 87.3% (many diffs)
8 human 100287171 WASHC1 WASH complex subunit 1 XM_011517663.3 90.6% 84.9% (many diffs)
9 human 100287171 WASHC1 WASH complex subunit 1 XM_017014169.1 90.6% 84.9% (many diffs)
10 human 100287171 WASHC1 WASH complex subunit 1 XM_011517664.3 90.4% 84.9% (many diffs)
11 human 100287171 WASHC1 WASH complex subunit 1 XM_017014170.1 90.4% 84.9% (many diffs)
12 human 100287171 WASHC1 WASH complex subunit 1 XM_011517666.2 87.6% 80.7% (many diffs)
13 human 102723897 WASH9P WAS protein family homolog ... XR_001737556.1 77.4% (many diffs)
14 human 100287171 WASHC1 WASH complex subunit 1 XM_017014171.1 77.2% 51.5% (many diffs)
15 human 100287171 WASHC1 WASH complex subunit 1 XM_017014172.1 77.2% 51.5% (many diffs)
16 human 100287171 WASHC1 WASH complex subunit 1 XR_002956737.1 75.8% (many diffs)
17 human 653635 WASH7P WASP family homolog 7, pseu... NR_024540.1 75% (many diffs)
18 human 100288778 WASH8P WAS protein family homolog ... NR_130745.1 72.1% (many diffs)
19 human 375260 WASH2P WASP family homolog 2, pseu... NR_024077.2 68% (many diffs)
20 human 100287171 WASHC1 WASH complex subunit 1 XR_002956741.1 66.4% (many diffs)
21 human 100287171 WASHC1 WASH complex subunit 1 XR_929142.2 64% (many diffs)
22 human 100287171 WASHC1 WASH complex subunit 1 XM_024447370.1 55.3% 54.4% (many diffs)
23 human 374666 WASH3P WASP family homolog 3, pseu... NR_003659.2 52% (many diffs)
24 human 100287171 WASHC1 WASH complex subunit 1 XM_017014173.1 49.5% 41.2% (many diffs)
25 human 100287171 WASHC1 WASH complex subunit 1 XR_002956738.1 36.8% (many diffs)
26 human 100287171 WASHC1 WASH complex subunit 1 XR_002956740.1 17.3% (many diffs)
27 human 100287171 WASHC1 WASH complex subunit 1 XR_002956739.1 17.2% (many diffs)
28 mouse 68767 Washc1 WASH complex subunit 1 NM_001037757.1 81.1% 84.4% (many diffs)
29 mouse 68767 Washc1 WASH complex subunit 1 NM_026833.1 81.1% 84.4% (many diffs)
30 mouse 68767 Washc1 WASH complex subunit 1 XM_006524859.1 81.1% 84.4% (many diffs)
31 mouse 68767 Washc1 WASH complex subunit 1 XM_006524860.3 81.1% 84.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1470
ORF length:
1404
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac tcctgtgagg atgcagcact ccctggcagg tcagacctat gccgtgcccc
121tcatccagcc agacctgcgg cgagaggagg ccgtccagca gatggcggat gccctgcagt
181acctgcagaa ggtctctgga gacatcttca gcaggatctc ccagcgggta gagcagagcc
241ggagccaggt gcaggccatt ggagagaagg tctccttggc ccaggccaag attgagaaga
301tcaagggcag caagaaggcc atcaaggtgt tctccagtgc caagtaccct gctccagagc
361gcctgcagga atatggctcc atcttcacgg gcgcccagga ccctggcctg cagagacgct
421cccgccacag gatccagagc aagcaccgcc ccctggacga gcgggccctg caggagaagc
481tgaagtactt tcctgtgtgt gtgagcacca agccggagcc cgaggacgat gcagaagagg
541gacttggggg tcttcccagc aacatcagct ctgtcagctc cttgctgctc ttcaacacca
601ccgagaacct gtacaagaag tatgtcttcc tggaccccct ggctggtgct gtaacaaaga
661cccatgtgat gctgggggca gagacagagg agaagctgtt tgatgccccc ttgtccatca
721gcaagagaga gcagctggaa cagcaggtcc cagagaacta cttctatgtg ccagacctgg
781gccaggtgcc tgagattgat gttccgtcct acctgcctga cctgcccggc attgccaacg
841acctcatgta cagtgccgac ctgggccccg gcattgcccc ctctgcccct ggcaccattc
901cggaactgcc caccttccac actgaggtag ccgagcctct caaggcagac ctacaagatg
961gggtactaac agcaccacca ccacccccac cgcccccacc acctccccca gctcctgagg
1021tgctggccag tgcatcccca ctcccacccT CAACCGCGGC CCCTGTAGGC CAAGGCGCCA
1081GGCAGGACGA CAGCAGCAGC AGCGCGTCTC CTTCAGTCCA GGGAGCTCCC AGGGAAGTGG
1141TCGACCCCTC CGGTGGCCGG GCCACTCTGC TAGAGTCCAT CCGCCAAGCT GGGGGCATCG
1201GCAAGGCCAA GCTGCGCAGC GTGAAGGAGC GAAAGCTGGA GAAGAAGAAG CAGAAGGAGC
1261AGGAGCAAGT GAGAGCCACG AGCCAAGGTG GGGACTTGAT GTCGGATCTC TTCAACAAGC
1321TGGTCATGAG GCGCAAGGGC ATCTCTGGGA AAGGACCTGG GGCTGGTGAG GGGCCCGGAG
1381GAGCCTTTGC CCGCGTGTCA GACTCCATCC CTCCTCTGCC GCCACCGCAG CAGCCACAGG
1441CAGAGGAGGA CGAGGACGAC TGGGAATCGT ACCCAACTTT CTTGTACAAA GTGGTTGATA
1501TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1561CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACATG
1621GAGTCAGTTC TTTTTGGCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1681tgaaagatt

Download FASTA (ORF) (Full)

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