Construct: ORF TRCN0000468571
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011269.1_s317c1
- Derived from:
- ccsbBroadEn_15113
- DNA Barcode:
- ACGACCGTCCCTTAAAGTGCGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPHK2 (56848)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468571
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56848 | SPHK2 | sphingosine kinase 2 | NM_001204159.3 | 100% | 100% | |
| 2 | human | 56848 | SPHK2 | sphingosine kinase 2 | NM_020126.5 | 100% | 100% | |
| 3 | human | 56848 | SPHK2 | sphingosine kinase 2 | XM_011527133.1 | 100% | 100% | |
| 4 | human | 56848 | SPHK2 | sphingosine kinase 2 | NM_001204160.3 | 94.4% | 94.4% | 0_1ins108 |
| 5 | human | 56848 | SPHK2 | sphingosine kinase 2 | XM_006723292.1 | 94.4% | 94.4% | 0_1ins108 |
| 6 | human | 56848 | SPHK2 | sphingosine kinase 2 | XM_011527134.1 | 94.4% | 94.4% | 0_1ins108 |
| 7 | human | 56848 | SPHK2 | sphingosine kinase 2 | NM_001204158.3 | 90.9% | 90.9% | 36_37ins177 |
| 8 | human | 56848 | SPHK2 | sphingosine kinase 2 | XM_017027008.1 | 89.3% | 89.8% | (many diffs) |
| 9 | human | 56848 | SPHK2 | sphingosine kinase 2 | NM_001243876.1 | 68.5% | 68.5% | 0_1ins618 |
| 10 | human | 56848 | SPHK2 | sphingosine kinase 2 | XM_017027010.2 | 54.3% | 46.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2028
- ORF length:
- 1962
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tggacacctt gaagcagagg agcagcagga ccagaggcca gaccaggagc 121 tgaccgggag ctggggccac gggcctagga gcaccctggt cagggctaag gccatggccc 181 cgcccccacc gccactggct gccagcaccc cgctcctcca tggcgagttt ggctcctacc 241 cagcccgagg cccacgcttt gccctcaccc ttacatcgca ggccctgcac atacagcggc 301 tgcgccccaa acctgaagcc aggccccggg gtggcctggt cccgttggcc gaggtctcag 361 gctgctgcac cctgcgaagc cgcagcccct cagactcagc ggcctacttc tgcatctaca 421 cctaccctcg gggccggcgc ggggcccggc gcagagccac tcgcaccttc cgggcagatg 481 gggccgccac ctacgaagag aaccgtgccg aggcccagcg ctgggccact gccctcacct 541 gtctgctccg aggactgcca ctgcccgggg atggggagat cacccctgac ctgctacctc 601 ggccgccccg gttgcttcta ttggtcaatc cctttggggg tcggggcctg gcctggcagt 661 ggtgtaagaa ccacgtgctt cccatgatct ctgaagctgg gctgtccttc aacctcatcc 721 agacagaacg acagaaccac gcccgggagc tggtccaggg gctgagcctg agtgagtggg 781 atggcatcgt cacggtctcg ggagacgggc tgctccatga ggtgctgaac gggctcctag 841 atcgccctga ctgggaggaa gctgtgaaga tgcctgtggg catcctcccc tgcggctcgg 901 gcaacgcgct ggccggagca gtgaaccagc acgggggatt tgagccagcc ctgggcctcg 961 acctgttgct caactgctca ctgttgctgt gccggggtgg tggccaccca ctggacctgc 1021 tctccgtgac gctggcctcg ggctcccgct gtttctcctt cctgtctgtg gcctggggct 1081 tcgtgtcaga tgtggatatc cagagcgagc gcttcagggc cttgggcagt gcccgcttca 1141 cactgggcac ggtgctgggc ctcgccacac tgcacaccta ccgcggacgc ctctcctacc 1201 tccccgccac tgtggaacct gcctcgccca cccctgccca tagcctgcct cgtgccaagt 1261 cggagctgac cctaacccca gacccagccc cgcccatggc ccactcaccc ctgcatcgtt 1321 ctgtgtctga cctgcctctt cccctgcccc agcctgccct ggcctctcct ggctcgccag 1381 aacccctgcc catcctgtcc ctcaacggtg ggggcccaga gctggctggg gactggggtg 1441 gggctgggga tgctccgctg tccccggacc cactgctgtc ttcacctcct ggctctccca 1501 aggcagctct acactcaccc gtctccgaag gggcccccgt aattccccca tcctctgggc 1561 tcccacttcc cacccctgat gcccgggtag gggcctccac ctgcggcccg cccgaccacc 1621 tgctgcctcc gctgggcacc ccgctgcccc cagactgggt gacgctggag ggggactttg 1681 tgctcatgtt ggccatctcg cccagccacc TAGGCGCTGA CCTGGTGGCA GCTCCGCATG 1741 CGCGCTTCGA CGACGGCCTG GTGCACCTGT GCTGGGTGCG TAGCGGCATC TCGCGGGCTG 1801 CGCTGCTGCG CCTTTTCTTG GCCATGGAGC GTGGTAGCCA CTTCAGCCTG GGCTGTCCGC 1861 AGCTGGGCTA CGCCGCGGCC CGTGCCTTCC GCCTAGAGCC GCTCACACCA CGCGGCGTGC 1921 TCACAGTGGA CGGGGAGCAG GTGGAGTATG GGCCGCTACA GGCACAGATG CACCCTGGCA 1981 TCGGTACACT GCTCACTGGG CCTCCTGGCT GCCCGGGGCG GGAGCCCTGC CCAACTTTCT 2041 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2101 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2161 GTGGAAAGGA CGAACGACCG TCCCTTAAAG TGCGCCCACG CGTTAAGTCg acaatcaacc 2221 tctggattac aaaatttgtg aaagatt