Construct: ORF TRCN0000468584
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004960.1_s317c1
- Derived from:
- ccsbBroadEn_03043
- DNA Barcode:
- CGATCCGATAACGGACCGGCAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEXI (28955)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468584
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28955 | DEXI | Dexi homolog | NM_014015.4 | 100% | 100% | |
| 2 | human | 100289656 | LOC100289656 | Dexi homolog (mouse) pseudo... | NR_036475.2 | 19.4% | 1_701del;879_880delTTinsCC;895_896ins91 | |
| 3 | mouse | 58239 | Dexi | dexamethasone-induced trans... | NM_021428.4 | 90.8% | 94.7% | (many diffs) |
| 4 | mouse | 58239 | Dexi | dexamethasone-induced trans... | XM_011245991.1 | 90.8% | 94.7% | (many diffs) |
| 5 | mouse | 58239 | Dexi | dexamethasone-induced trans... | XM_017317106.1 | 90.8% | 94.7% | (many diffs) |
| 6 | mouse | 58239 | Dexi | dexamethasone-induced trans... | XM_017317107.1 | 90.8% | 94.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 375
- ORF length:
- 285
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCTCGGCGC CCGGGTCGCG GCCCACCTGG 121 ACGCACTGGG CCCCCTGGTC CCCTACGTGC CGCCGCCGCT GCTGCCCTCT ATGTTCTACG 181 TGGGCCTGTT CTTCGTCAAT GTGCTGATCC TGTACTACGC CTTCCTCATG GAGTACATCG 241 TCCTCAACGT GGGCCTCGTC TTCCTGCCCG AGGACATGGA CCAGGCGCTC GTGGACCTCG 301 GCGTGCTCTC CGACCCCGGC TCGGGCCTTT ACGATGCTGA CTCGGAGCTC GACGTCTTTG 361 ATGCGTACTT GGAGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 421 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 481 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGATCCGATA ACGGACCGGC 541 AGTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt