Construct: ORF TRCN0000468616
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017119.1_s317c1
- Derived from:
- ccsbBroadEn_03308
- DNA Barcode:
- CAACCGAAACATCCGAAGCTCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YTHDF2 (51441)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468616
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51441 | YTHDF2 | YTH N6-methyladenosine RNA ... | NM_001173128.1 | 100% | 100% | |
| 2 | human | 51441 | YTHDF2 | YTH N6-methyladenosine RNA ... | NM_016258.3 | 100% | 100% | |
| 3 | human | 51441 | YTHDF2 | YTH N6-methyladenosine RNA ... | NM_001172828.2 | 91.3% | 91.3% | 0_1ins150 |
| 4 | mouse | 213541 | Ythdf2 | YTH domain family 2 | NM_145393.4 | 95.6% | 99.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1803
- ORF length:
- 1737
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggccagcagc ctcttggagc agagaccaaa aggtcaagga aacaaagtac 121 aaaatggatc tgtacatcaa aaggatggat taaacgatga tgattttgaa ccttacttga 181 gtccacaggc aaggcccaat aatgcatata ctgccatgtc agattcctac ttacccagtt 241 actacagtcc ctccattggc ttctcctatt ctttgggtga agctgcttgg tctacggggg 301 gtgacacagc catgccctac ttaacttctt atggacagct gagcaacgga gagccccact 361 tcctaccaga tgcaatgttt gggcaaccag gagccctagg tagcactcca tttcttggtc 421 agcatggttt taatttcttt cccagtggga ttgacttctc agcatgggga aataacagtt 481 ctcagggaca gtctactcag agctctggat atagtagcaa ttatgcttat gcacctagct 541 ccttaggtgg agccatgatt gatggacagt cagcttttgc caatgagacc ctcaataagg 601 ctcctggcat gaatactata gaccaaggga tggcagcact gaagttgggt agcacagaag 661 ttgcaagcaa tgttccaaaa gttgtaggtt ctgctgttgg tagcgggtcc attactagta 721 acatcgtggc ttccaatagt ttgcctccag ccaccattgc tcctccaaaa ccagcatctt 781 gggctgatat tgctagcaag cctgcaaaac agcaacctaa actgaagacc aagaatggca 841 ttgcagggtc aagtcttccg ccacccccga taaagcataa catggatatt ggaacttggg 901 ataacaaggg tcccgttgca aaagccccct cacaggcttt ggttcagaat ataggtcagc 961 caacccaggg gtctcctcag cctgtaggtc agcaggctaa caatagccca ccagtggctc 1021 aggcatcagt agggcaacag acacagccat tgcctccacc tccaccacag cctgcccagc 1081 tttcagtcca gcaacaggca gctcagccaa cccgctgggt agcacctcgg aaccgtggca 1141 gtgggttcgg tcataatggg gtggatggta atggagtagg acagtctcag gctggttctg 1201 gatctactcc ttcagaaccc cacccagtgt tggagaagct tcggtccatt aataactata 1261 accccaaaga ttttgactgg aatctgaaac atggccgggt tttcatcatt aagagctact 1321 ctgaggacga tattcaccgt tccattaagt ataatatttg gtgcagcaca gagcatggta 1381 acaagagact ggatgctgct tatcgttcca tgaacgggaa aggccccgtt tacttacttt 1441 tcagtgtcaa cggcagtgga cacttctgtg gcgtggcaga aatgaaaTCT GCTGTGGACT 1501 ACAACACATG TGCAGGTGTG TGGTCCCAGG ACAAATGGAA GGGTCGTTTT GATGTCAGGT 1561 GGATTTTTGT GAAGGACGTT CCCAATAGCC AACTGCGACA CATTCGCCTA GAGAACAACG 1621 AGAATAAACC AGTGACCAAC TCTAGGGACA CTCAGGAAGT GCCTCTGGAA AAGGCTAAGC 1681 AGGTGTTGAA AATTATAGCC AGCTACAAGC ACACCACTTC CATTTTTGAT GACTTCTCAC 1741 ACTATGAGAA ACGCCAAGAG GAAGAAGAAA GTGTTAAAAA GGAACGTCAA GGTCGTGGGA 1801 AATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1861 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1921 GGCTTTATAT ATCTTGTGGA AAGGACGACA ACCGAAACAT CCGAAGCTCA AGACGCGTTA 1981 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt