Construct: ORF TRCN0000468620

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016286.1_s317c1
Derived from:
ccsbBroadEn_03794
DNA Barcode:
AGCCAACAGCAGTTTGATTGCGGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RTN4 (57142)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468620
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57142 RTN4 reticulon 4 NM_153828.3 100% 100%
2 human 57142 RTN4 reticulon 4 NM_207520.2 95.1% 95.1% 556_612del
3 human 57142 RTN4 reticulon 4 NM_007008.3 50.4% 50.4% (many diffs)
4 human 57142 RTN4 reticulon 4 NM_020532.5 31.2% 31.2% 556_3012del
5 human 57142 RTN4 reticulon 4 NR_135829.1 30% (many diffs)
6 human 57142 RTN4 reticulon 4 NR_135830.2 27.7% (many diffs)
7 mouse 68585 Rtn4 reticulon 4 NM_194052.3 84.8% 87.4% (many diffs)
8 mouse 68585 Rtn4 reticulon 4 NM_194053.3 80.7% 82.9% (many diffs)
9 mouse 68585 Rtn4 reticulon 4 NM_024226.4 46.9% 50.1% (many diffs)
10 mouse 68585 Rtn4 reticulon 4 NM_194054.3 28.2% 27.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1185
ORF length:
1119
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga agacctggac cagtctcctc tggtctcgtc ctcggacagc ccaccccggc
121cgcagcccgc gttcaagtac cagttcgtga gggagcccga ggacgaggag gaagaagagg
181aggaggaaga ggaggacgag gacgaagacc tggaggagct ggaggtgctg gagaggaagc
241ccgccgccgg gctgtccgcg gccccagtgc ccaccgcccc tgccgccggc gcgcccctga
301tggacttcgg aaatgacttc gtgccgccgg cgccccgggg acccctgccg gccgctcccc
361ccgtcgcccc ggagcggcag ccgtcttggg acccgagccc ggtgtcgtcg accgtgcccg
421cgccatcccc gctgtctgct gccgcagtct cgccctccaa gctccctgag gacgacgagc
481ctccggcccg gcctccccct cctcccccgg ccagcgtgag cccccaggca gagcccgtgt
541ggaccccgcc agccccggct cccgccgcgc ccccctccac cccggccgcg cccaagcgca
601ggggctcctc gggctcagtg gttgttgacc tcctgtactg gagagacatt aagaagactg
661gagtggtgtt tggtgccagc ctattcctgc tgctttcatt gacagtattc agcattgtga
721gcgtaacagc ctacattgcc ttggccctgc tctctgtgac catcagcttt aggatataca
781agggtgtgat ccaagctatc cagaaatcag atgaaggCCA CCCATTCAGG GCATATCTGG
841AATCTGAAGT TGCTATATCT GAGGAGTTGG TTCAGAAGTA CAGTAATTCT GCTCTTGGTC
901ATGTGAACTG CACGATAAAG GAACTCAGGC GCCTCTTCTT AGTTGATGAT TTAGTTGATT
961CTCTGAAGTT TGCAGTGTTG ATGTGGGTAT TTACCTATGT TGGTGCCTTG TTTAATGGTC
1021TGACACTACT GATTTTGGCT CTCATTTCAC TCTTCAGTGT TCCTGTTATT TATGAACGGC
1081ATCAGGCACA GATAGATCAT TATCTAGGAC TTGCAAATAA GAATGTTAAA GATGCTATGG
1141CTAAAATCCA AGCAAAAATC CCTGGATTGA AGCGCAAAGC TGAATGCCCA ACTTTCTTGT
1201ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1261AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1321GAAAGGACGA AGCCAACAGC AGTTTGATTG CGGTACGCGT TAAGTCgaca atcaacctct
1381ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)