Construct: ORF TRCN0000468620
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016286.1_s317c1
- Derived from:
- ccsbBroadEn_03794
- DNA Barcode:
- AGCCAACAGCAGTTTGATTGCGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTN4 (57142)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468620
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57142 | RTN4 | reticulon 4 | NM_153828.3 | 100% | 100% | |
| 2 | human | 57142 | RTN4 | reticulon 4 | NM_207520.2 | 95.1% | 95.1% | 556_612del |
| 3 | human | 57142 | RTN4 | reticulon 4 | NM_007008.3 | 50.4% | 50.4% | (many diffs) |
| 4 | human | 57142 | RTN4 | reticulon 4 | NM_020532.5 | 31.2% | 31.2% | 556_3012del |
| 5 | human | 57142 | RTN4 | reticulon 4 | NR_135829.1 | 30% | (many diffs) | |
| 6 | human | 57142 | RTN4 | reticulon 4 | NR_135830.2 | 27.7% | (many diffs) | |
| 7 | mouse | 68585 | Rtn4 | reticulon 4 | NM_194052.3 | 84.8% | 87.4% | (many diffs) |
| 8 | mouse | 68585 | Rtn4 | reticulon 4 | NM_194053.3 | 80.7% | 82.9% | (many diffs) |
| 9 | mouse | 68585 | Rtn4 | reticulon 4 | NM_024226.4 | 46.9% | 50.1% | (many diffs) |
| 10 | mouse | 68585 | Rtn4 | reticulon 4 | NM_194054.3 | 28.2% | 27.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1185
- ORF length:
- 1119
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agacctggac cagtctcctc tggtctcgtc ctcggacagc ccaccccggc 121 cgcagcccgc gttcaagtac cagttcgtga gggagcccga ggacgaggag gaagaagagg 181 aggaggaaga ggaggacgag gacgaagacc tggaggagct ggaggtgctg gagaggaagc 241 ccgccgccgg gctgtccgcg gccccagtgc ccaccgcccc tgccgccggc gcgcccctga 301 tggacttcgg aaatgacttc gtgccgccgg cgccccgggg acccctgccg gccgctcccc 361 ccgtcgcccc ggagcggcag ccgtcttggg acccgagccc ggtgtcgtcg accgtgcccg 421 cgccatcccc gctgtctgct gccgcagtct cgccctccaa gctccctgag gacgacgagc 481 ctccggcccg gcctccccct cctcccccgg ccagcgtgag cccccaggca gagcccgtgt 541 ggaccccgcc agccccggct cccgccgcgc ccccctccac cccggccgcg cccaagcgca 601 ggggctcctc gggctcagtg gttgttgacc tcctgtactg gagagacatt aagaagactg 661 gagtggtgtt tggtgccagc ctattcctgc tgctttcatt gacagtattc agcattgtga 721 gcgtaacagc ctacattgcc ttggccctgc tctctgtgac catcagcttt aggatataca 781 agggtgtgat ccaagctatc cagaaatcag atgaaggCCA CCCATTCAGG GCATATCTGG 841 AATCTGAAGT TGCTATATCT GAGGAGTTGG TTCAGAAGTA CAGTAATTCT GCTCTTGGTC 901 ATGTGAACTG CACGATAAAG GAACTCAGGC GCCTCTTCTT AGTTGATGAT TTAGTTGATT 961 CTCTGAAGTT TGCAGTGTTG ATGTGGGTAT TTACCTATGT TGGTGCCTTG TTTAATGGTC 1021 TGACACTACT GATTTTGGCT CTCATTTCAC TCTTCAGTGT TCCTGTTATT TATGAACGGC 1081 ATCAGGCACA GATAGATCAT TATCTAGGAC TTGCAAATAA GAATGTTAAA GATGCTATGG 1141 CTAAAATCCA AGCAAAAATC CCTGGATTGA AGCGCAAAGC TGAATGCCCA ACTTTCTTGT 1201 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1261 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1321 GAAAGGACGA AGCCAACAGC AGTTTGATTG CGGTACGCGT TAAGTCgaca atcaacctct 1381 ggattacaaa atttgtgaaa gatt