Transcript: Human NM_007008.3

Homo sapiens reticulon 4 (RTN4), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-27
Taxon:
Homo sapiens (human)
Gene:
RTN4 (57142)
Length:
1719
CDS:
153..752

Additional Resources:

NCBI RefSeq record:
NM_007008.3
NBCI Gene record:
RTN4 (57142)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_007008.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000417227 GTATGGATTTAAACCGTAATC pLKO_005 964 3UTR 100% 10.800 15.120 N RTN4 n/a
2 TRCN0000420965 AGACGAGATCATACCGGTAAA pLKO_005 1186 3UTR 100% 10.800 8.640 N RTN4 n/a
3 TRCN0000071688 GCAGTGTTGATGTGGGTATTT pLKO.1 537 CDS 100% 13.200 9.240 N Rtn4 n/a
4 TRCN0000179649 GCAGTGTTGATGTGGGTATTT pLKO.1 537 CDS 100% 13.200 9.240 N RTN4 n/a
5 TRCN0000183585 GCTGCTTTCATTGACAGTATT pLKO.1 254 CDS 100% 13.200 9.240 N RTN4 n/a
6 TRCN0000147624 GCATATCTGGAATCTGAAGTT pLKO.1 396 CDS 100% 4.950 3.465 N RTN4 n/a
7 TRCN0000179762 GCTATATCTGAGGAGTTGGTT pLKO.1 417 CDS 100% 3.000 2.100 N RTN4 n/a
8 TRCN0000147846 GAAGTACAGTAATTCTGCTCT pLKO.1 440 CDS 100% 2.640 1.848 N RTN4 n/a
9 TRCN0000146691 CCTGTTATTTATGAACGGCAT pLKO.1 627 CDS 100% 2.160 1.512 N RTN4 n/a
10 TRCN0000435974 AGTTGATGATTTAGTTGATTC pLKO_005 506 CDS 100% 10.800 6.480 N RTN4 n/a
11 TRCN0000375427 CCACCCATTCAGGGCATATTT pLKO_005 383 CDS 100% 15.000 21.000 N Rtn4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_007008.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15937 pDONR223 0% 100% 100% None n/a
2 ccsbBroadEn_15938 pDONR223 0% 54.7% 54.8% None (many diffs) n/a
3 ccsbBroadEn_03794 pDONR223 100% 50.4% 50.4% None (many diffs) n/a
4 ccsbBroad304_03794 pLX_304 0% 50.4% 50.4% V5 (many diffs) n/a
5 TRCN0000468620 AGCCAACAGCAGTTTGATTGCGGT pLX_317 33.1% 50.4% 50.4% V5 (many diffs) n/a
6 ccsbBroadEn_03793 pDONR223 100% 49.1% 47.9% None (many diffs) n/a
7 ccsbBroad304_03793 pLX_304 0% 49.1% 47.9% V5 (many diffs) n/a
8 TRCN0000473077 TTAAATCCGCAGGACACTGCGCTG pLX_317 37.4% 49.1% 47.9% V5 (many diffs) n/a
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