Transcript: Mouse NM_024226.4

Mus musculus reticulon 4 (Rtn4), transcript variant 4, mRNA.

Source:
NCBI, updated 2017-06-26
Taxon:
Mus musculus (mouse)
Gene:
Rtn4 (68585)
Length:
3271
CDS:
287..886

Additional Resources:

NCBI RefSeq record:
NM_024226.4
NBCI Gene record:
Rtn4 (68585)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_024226.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000375427 CCACCCATTCAGGGCATATTT pLKO_005 517 CDS 100% 15.000 21.000 N Rtn4 n/a
2 TRCN0000379233 CAGGCGCAGATAGATCATTAT pLKO_005 782 CDS 100% 13.200 10.560 N Rtn4 n/a
3 TRCN0000366604 ACTATCAGCTTTAGGATATAT pLKO_005 458 CDS 100% 15.000 10.500 N Rtn4 n/a
4 TRCN0000375501 ACTGTTAGATTGCCAATATAA pLKO_005 1363 3UTR 100% 15.000 10.500 N Rtn4 n/a
5 TRCN0000071688 GCAGTGTTGATGTGGGTATTT pLKO.1 671 CDS 100% 13.200 9.240 N Rtn4 n/a
6 TRCN0000179649 GCAGTGTTGATGTGGGTATTT pLKO.1 671 CDS 100% 13.200 9.240 N RTN4 n/a
7 TRCN0000435974 AGTTGATGATTTAGTTGATTC pLKO_005 640 CDS 100% 10.800 7.560 N RTN4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_024226.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15937 pDONR223 0% 92.7% 97.4% None (many diffs) n/a
2 ccsbBroadEn_15938 pDONR223 0% 50.9% 54.5% None (many diffs) n/a
3 ccsbBroadEn_03794 pDONR223 100% 46.9% 50.1% None (many diffs) n/a
4 ccsbBroad304_03794 pLX_304 0% 46.9% 50.1% V5 (many diffs) n/a
5 TRCN0000468620 AGCCAACAGCAGTTTGATTGCGGT pLX_317 33.1% 46.9% 50.1% V5 (many diffs) n/a
6 ccsbBroadEn_03793 pDONR223 100% 46.5% 47.1% None (many diffs) n/a
7 ccsbBroad304_03793 pLX_304 0% 46.5% 47.1% V5 (many diffs) n/a
8 TRCN0000473077 TTAAATCCGCAGGACACTGCGCTG pLX_317 37.4% 46.5% 47.1% V5 (many diffs) n/a
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