Construct: ORF TRCN0000468634
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006535.1_s317c1
- Derived from:
- ccsbBroadEn_01433
- DNA Barcode:
- ACAACCATTAGTATCTCTTACACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL31 (6160)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468634
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6160 | RPL31 | ribosomal protein L31 | NM_000993.5 | 100% | 100% | |
| 2 | human | 6160 | RPL31 | ribosomal protein L31 | NM_001098577.2 | 93.3% | 92.1% | (many diffs) |
| 3 | human | 6160 | RPL31 | ribosomal protein L31 | NM_001099693.1 | 93.1% | 92% | (many diffs) |
| 4 | human | 641311 | RPL31P11 | ribosomal protein L31 pseud... | NR_002595.1 | 22.2% | (many diffs) | |
| 5 | mouse | 114641 | Rpl31 | ribosomal protein L31 | NM_001252218.1 | 90.9% | 100% | (many diffs) |
| 6 | mouse | 114641 | Rpl31 | ribosomal protein L31 | NM_001252219.1 | 90.9% | 100% | (many diffs) |
| 7 | mouse | 114641 | Rpl31 | ribosomal protein L31 | NM_053257.3 | 90.9% | 100% | (many diffs) |
| 8 | mouse | 665562 | Rpl31-ps12 | ribosomal protein L31, pseu... | NM_001258458.1 | 77.9% | 84.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 79
- ORF end:
- 454
- ORF length:
- 375
- Sequence:
-
1 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT 61 TTGTACAAAA AAGTTGGCAT GGCTCCCGCA AAGAAGGGTG GCGAGAAGAA AAAGGGCCGT 121 TCTGCCATCA ACGAAGTGGT AACCCGAGAA TACACCATCA ACATTCACAA GCGCATCCAT 181 GGAGTGGGCT TCAAGAAGCG TGCACCTCGG GCACTCAAAG AGATTCGGAA ATTTGCCATG 241 AAGGAGATGG GAACTCCAGA TGTGCGCATT GACACCAGGC TCAACAAAGC TGTCTGGGCC 301 AAAGGAATAA GGAATGTGCC ATACCGAATC CGTGTGCGGC TGTCCAGAAA ACGTAATGAG 361 GATGAAGATT CACCAAATAA GCTATATACT TTGGTTACCT ATGTACCTGT TACCACTTTC 421 AAAAATCTAC AGACAGTCAA TGTGGATGAG AACTACCCAA CTTTCTTGTA CAAAGTGGTT 481 GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT 541 AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA 601 CAACCATTAG TATCTCTTAC ACAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa 661 tttgtgaaag att