Construct: ORF TRCN0000468644

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016524.1_s317c1
Derived from:
ccsbBroadEn_02751
DNA Barcode:
GTGCTTGGTGTGTATATTGGTGTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SATB2 (23314)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468644
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23314 SATB2 SATB homeobox 2 NM_001172509.2 100% 100%
2 human 23314 SATB2 SATB homeobox 2 NM_001172517.1 100% 100%
3 human 23314 SATB2 SATB homeobox 2 NM_015265.4 100% 100%
4 human 23314 SATB2 SATB homeobox 2 XM_011510840.3 100% 100%
5 human 23314 SATB2 SATB homeobox 2 XM_005246396.3 92% 92% 0_1ins174
6 human 23314 SATB2 SATB homeobox 2 XM_017003656.1 92% 92% 0_1ins174
7 human 23314 SATB2 SATB homeobox 2 XM_024452767.1 80.7% 80.7% 0_1ins423
8 human 23314 SATB2 SATB homeobox 2 XM_024452768.1 80.7% 80.7% 0_1ins423
9 mouse 212712 Satb2 special AT-rich sequence bi... NM_139146.2 92.4% 99.5% (many diffs)
10 mouse 212712 Satb2 special AT-rich sequence bi... XM_017319630.1 92.4% 99.5% (many diffs)
11 mouse 212712 Satb2 special AT-rich sequence bi... XM_017319631.1 92.4% 99.5% (many diffs)
12 mouse 212712 Satb2 special AT-rich sequence bi... XM_006495827.3 91.5% 98.6% (many diffs)
13 mouse 212712 Satb2 special AT-rich sequence bi... XM_011238467.2 91.5% 98.6% (many diffs)
14 mouse 212712 Satb2 special AT-rich sequence bi... XM_017319629.1 91.5% 98.6% (many diffs)
15 mouse 212712 Satb2 special AT-rich sequence bi... XM_006495828.3 84.7% 91.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2268
ORF length:
2199
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggagcggcgg agcgagagcc cgtgtctgcg ggacagcccc gaccggcgga
121gcggcagccc ggacgtcaag gggcctcccc cagtgaaggt ggcccggctg gagcagaacg
181gcagccccat gggagcccgc gggaggccca acggcgccgt ggccaaggcc gtgggaggtt
241tgatgattcc tgtcttttgt gtcgtggagc agttggacgg ctctcttgaa tatgacaaca
301gagaagaaca cgccgagttt gtcctggtgc ggaaagatgt gctttttagc cagctggtgg
361agactgcgct cctggccctg gggtattctc acagctctgc ggcccaggcc caaggaataa
421tcaagctggg aaggtggaac cctctccccc tcagttatgt gacagatgca cccgacgcga
481cagtggccga catgctacaa gatgtctatc atgttgtgac gttgaaaatc caattacaaa
541gttgttcaaa gttggaagac ttgcctgcgg agcagtggaa ccatgccaca gtccgcaatg
601ccttaaagga actgctcaaa gagatgaacc agagcacatt agccaaagaa tgccctctct
661cccagagtat gatttcatcc attgtaaata gcacatatta tgccaatgtg tcagcaacca
721agtgccagga gtttgggaga tggtataaaa agtacaagaa gattaaagtg gaaagagtgg
781aacgagaaaa cctttcagac tattgtgttc tgggccagcg tccaatgcat ttaccaaata
841tgaaccagct ggcatccctg gggaaaacca acgaacagtc tcctcacagc caaattcacc
901acagtactcc aatccgaaac caagtgcccg cattacagcc catcatgagc cctggtcttc
961tttctcccca gcttagtcca caacttgtaa ggcaacaaat agccatggcc catctgataa
1021accaacagat tgccgttagc cggctcctgg ctcaccagca tcctcaagcc atcaaccagc
1081agttcctgaa ccatccaccc atccccagag cagttaagcc agagccaacc aactcttccg
1141tggaagtctc tccagatatc taccagcaag tcagagatga gctgaagagg gccagtgtgt
1201cccaagctgt ctttgcaaga gtggcattca accgcacaca gggattgttg tctgagattc
1261tgcgtaagga agaagaccct cggacagcct ctcagtctct tctagtaaac ctgagggcca
1321tgcagaattt cctcaatctg ccagaagtgg agcgagatcg catctaccag gatgagaggg
1381agcggagcat gaatcccaat gtgagcatgg tctcctcggc ctccagcagt cccagctcct
1441cccgaacccc tcaggccaaa acctcgacac cgacaacaga cctccctatt aaggtggacg
1501gcgccaacat caacatcaca gctgccattt atgacgagat ccaacaggag atgaaaaggg
1561ccaaggtgtc tcaagccctg tttgccaaag tggctgcaaa taaaagtcag ggctggctgt
1621gtgaactgct ccgctggaag gagaacccaa gcccagaaaa ccgcaccctc tgggaaaacc
1681tctgtaccat ccgtcgcttc ctgaaccttc cccagcatga gagggatgtc atctatgagg
1741aggagtcaag gcatcaccac agcgaacgca tgcaacacgt ggtccagctt ccccctgagc
1801cggtgcaggt acttcataga cagcagtctc agccagccaa ggagagttcc cctcccagag
1861aagaagcgcc tcccccacct ccTCCGACTG AAGACAGTTG TGCCAAAAAG CCCCGGTCTC
1921GCACAAAGAT CTCCTTAGAA GCCCTGGGGA TCCTCCAAAG CTTTATTCAT GATGTAGGCC
1981TGTACCCAGA CCAGGAAGCC ATCCACACTC TTTCGGCTCA GCTGGATCTC CCCAAACACA
2041CCATCATCAA GTTCTTCCAG AACCAGCGGT ACCACGTGAA GCACCACGGG AAGCTGAAAG
2101AGCACCTGGG CTCCGCGGTG GACGTGGCTG AATATAAGGA CGAGGAGCTG CTGACCGAGT
2161CAGAGGAGAA CGACAGCGAG GAAGGCTCCG AGGAGATGTA CAAAGTGGAG GCTGAGGAGG
2221AAAATGCTGA CAAAAGCAAG GCAGCACCTG CCGAAATTGA CCAGAGATTG CCAACTTTCT
2281TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
2341CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
2401GTGGAAAGGA CGAGTGCTTG GTGTGTATAT TGGTGTAACG CGTTAAGTCg acaatcaacc
2461tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)