Construct: ORF TRCN0000468644
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016524.1_s317c1
- Derived from:
- ccsbBroadEn_02751
- DNA Barcode:
- GTGCTTGGTGTGTATATTGGTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SATB2 (23314)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468644
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23314 | SATB2 | SATB homeobox 2 | NM_001172509.2 | 100% | 100% | |
| 2 | human | 23314 | SATB2 | SATB homeobox 2 | NM_001172517.1 | 100% | 100% | |
| 3 | human | 23314 | SATB2 | SATB homeobox 2 | NM_015265.4 | 100% | 100% | |
| 4 | human | 23314 | SATB2 | SATB homeobox 2 | XM_011510840.3 | 100% | 100% | |
| 5 | human | 23314 | SATB2 | SATB homeobox 2 | XM_005246396.3 | 92% | 92% | 0_1ins174 |
| 6 | human | 23314 | SATB2 | SATB homeobox 2 | XM_017003656.1 | 92% | 92% | 0_1ins174 |
| 7 | human | 23314 | SATB2 | SATB homeobox 2 | XM_024452767.1 | 80.7% | 80.7% | 0_1ins423 |
| 8 | human | 23314 | SATB2 | SATB homeobox 2 | XM_024452768.1 | 80.7% | 80.7% | 0_1ins423 |
| 9 | mouse | 212712 | Satb2 | special AT-rich sequence bi... | NM_139146.2 | 92.4% | 99.5% | (many diffs) |
| 10 | mouse | 212712 | Satb2 | special AT-rich sequence bi... | XM_017319630.1 | 92.4% | 99.5% | (many diffs) |
| 11 | mouse | 212712 | Satb2 | special AT-rich sequence bi... | XM_017319631.1 | 92.4% | 99.5% | (many diffs) |
| 12 | mouse | 212712 | Satb2 | special AT-rich sequence bi... | XM_006495827.3 | 91.5% | 98.6% | (many diffs) |
| 13 | mouse | 212712 | Satb2 | special AT-rich sequence bi... | XM_011238467.2 | 91.5% | 98.6% | (many diffs) |
| 14 | mouse | 212712 | Satb2 | special AT-rich sequence bi... | XM_017319629.1 | 91.5% | 98.6% | (many diffs) |
| 15 | mouse | 212712 | Satb2 | special AT-rich sequence bi... | XM_006495828.3 | 84.7% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2268
- ORF length:
- 2199
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcggcgg agcgagagcc cgtgtctgcg ggacagcccc gaccggcgga 121 gcggcagccc ggacgtcaag gggcctcccc cagtgaaggt ggcccggctg gagcagaacg 181 gcagccccat gggagcccgc gggaggccca acggcgccgt ggccaaggcc gtgggaggtt 241 tgatgattcc tgtcttttgt gtcgtggagc agttggacgg ctctcttgaa tatgacaaca 301 gagaagaaca cgccgagttt gtcctggtgc ggaaagatgt gctttttagc cagctggtgg 361 agactgcgct cctggccctg gggtattctc acagctctgc ggcccaggcc caaggaataa 421 tcaagctggg aaggtggaac cctctccccc tcagttatgt gacagatgca cccgacgcga 481 cagtggccga catgctacaa gatgtctatc atgttgtgac gttgaaaatc caattacaaa 541 gttgttcaaa gttggaagac ttgcctgcgg agcagtggaa ccatgccaca gtccgcaatg 601 ccttaaagga actgctcaaa gagatgaacc agagcacatt agccaaagaa tgccctctct 661 cccagagtat gatttcatcc attgtaaata gcacatatta tgccaatgtg tcagcaacca 721 agtgccagga gtttgggaga tggtataaaa agtacaagaa gattaaagtg gaaagagtgg 781 aacgagaaaa cctttcagac tattgtgttc tgggccagcg tccaatgcat ttaccaaata 841 tgaaccagct ggcatccctg gggaaaacca acgaacagtc tcctcacagc caaattcacc 901 acagtactcc aatccgaaac caagtgcccg cattacagcc catcatgagc cctggtcttc 961 tttctcccca gcttagtcca caacttgtaa ggcaacaaat agccatggcc catctgataa 1021 accaacagat tgccgttagc cggctcctgg ctcaccagca tcctcaagcc atcaaccagc 1081 agttcctgaa ccatccaccc atccccagag cagttaagcc agagccaacc aactcttccg 1141 tggaagtctc tccagatatc taccagcaag tcagagatga gctgaagagg gccagtgtgt 1201 cccaagctgt ctttgcaaga gtggcattca accgcacaca gggattgttg tctgagattc 1261 tgcgtaagga agaagaccct cggacagcct ctcagtctct tctagtaaac ctgagggcca 1321 tgcagaattt cctcaatctg ccagaagtgg agcgagatcg catctaccag gatgagaggg 1381 agcggagcat gaatcccaat gtgagcatgg tctcctcggc ctccagcagt cccagctcct 1441 cccgaacccc tcaggccaaa acctcgacac cgacaacaga cctccctatt aaggtggacg 1501 gcgccaacat caacatcaca gctgccattt atgacgagat ccaacaggag atgaaaaggg 1561 ccaaggtgtc tcaagccctg tttgccaaag tggctgcaaa taaaagtcag ggctggctgt 1621 gtgaactgct ccgctggaag gagaacccaa gcccagaaaa ccgcaccctc tgggaaaacc 1681 tctgtaccat ccgtcgcttc ctgaaccttc cccagcatga gagggatgtc atctatgagg 1741 aggagtcaag gcatcaccac agcgaacgca tgcaacacgt ggtccagctt ccccctgagc 1801 cggtgcaggt acttcataga cagcagtctc agccagccaa ggagagttcc cctcccagag 1861 aagaagcgcc tcccccacct ccTCCGACTG AAGACAGTTG TGCCAAAAAG CCCCGGTCTC 1921 GCACAAAGAT CTCCTTAGAA GCCCTGGGGA TCCTCCAAAG CTTTATTCAT GATGTAGGCC 1981 TGTACCCAGA CCAGGAAGCC ATCCACACTC TTTCGGCTCA GCTGGATCTC CCCAAACACA 2041 CCATCATCAA GTTCTTCCAG AACCAGCGGT ACCACGTGAA GCACCACGGG AAGCTGAAAG 2101 AGCACCTGGG CTCCGCGGTG GACGTGGCTG AATATAAGGA CGAGGAGCTG CTGACCGAGT 2161 CAGAGGAGAA CGACAGCGAG GAAGGCTCCG AGGAGATGTA CAAAGTGGAG GCTGAGGAGG 2221 AAAATGCTGA CAAAAGCAAG GCAGCACCTG CCGAAATTGA CCAGAGATTG CCAACTTTCT 2281 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2341 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2401 GTGGAAAGGA CGAGTGCTTG GTGTGTATAT TGGTGTAACG CGTTAAGTCg acaatcaacc 2461 tctggattac aaaatttgtg aaagatt