Construct: ORF TRCN0000468656
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016924.1_s317c1
- Derived from:
- ccsbBroadEn_04381
- DNA Barcode:
- GTTCCTGAATCCCGGAATTACAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCRIP2 (84331)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468656
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NM_138418.4 | 100% | 100% | |
| 2 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NM_001331229.2 | 78.7% | 78.1% | 310_311ins102 |
| 3 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NR_138605.2 | 50.1% | (many diffs) | |
| 4 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NR_138610.1 | 49.1% | (many diffs) | |
| 5 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NR_138609.1 | 49.1% | (many diffs) | |
| 6 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NM_001331230.2 | 46.2% | 46.2% | 50_51ins258 |
| 7 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NR_138607.2 | 45.8% | (many diffs) | |
| 8 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NR_138608.2 | 45.1% | 1_164del;347_479del;778_1064del | |
| 9 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NR_138604.2 | 44.9% | 1_164del;345_481del;782_1068del | |
| 10 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NR_138611.1 | 44.1% | (many diffs) | |
| 11 | human | 84331 | MCRIP2 | MAPK regulated corepressor ... | NR_138606.2 | 37.8% | 1_164del;344_345ins128;517_803del | |
| 12 | mouse | 68241 | Mcrip2 | MAPK regulated corepressor ... | NM_026633.3 | 85.8% | 85% | (many diffs) |
| 13 | mouse | 68241 | Mcrip2 | MAPK regulated corepressor ... | XM_006524838.2 | 78% | 76.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 546
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta caccatcacc aaggggccca gcaagctggt cgcgcagcgc cgcacaggTC 121 CCACGCAGCA GCAGGTGGAG GGCCGGCTCG GCGAGCTCCT GAAATGCCGG CAGCCCGCGC 181 CGCCGACCTC GCAGCCCCCG CGGGCGCAGC CCTTTGCGCA GCCGCCGGGA CCCTGGCCCC 241 TGTCGAGTCC AGGGCCAAGG CTTGTGTTCA ATCGTGTGAA TGGCCGGCGG GCCCCCTCCA 301 CGTCCCCATC CTTCGAGGGG ACCCAGGAGA CCTACACAGT GGCCCACGAG GAGAATGTCC 361 GCTTTGTGTC CGAAGCCTGG CAGCAGGTGC AACAGCAGCT GGATGGTGGC CCAGCCGGTG 421 AGGGCGGGCC AAGGCCTGTG CAGTACGTGG AGAGGACCCC CAATCCCCGG CTGCAGAACT 481 TTGTGCCCAT TGACCTAGAC GAGTGGTGGG CGCAGCAGTT CCTGGCGAGA ATCACCAGCT 541 GTTCCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 601 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 661 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTTCCTGAA TCCCGGAATT ACAAAACGCG 721 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt