Construct: ORF TRCN0000468662
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008749.1_s317c1
- Derived from:
- ccsbBroadEn_01226
- DNA Barcode:
- GGACCCTTACTTCTTAAAGTAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNOC (5368)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468662
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5368 | PNOC | prepronociceptin | NM_006228.5 | 100% | 100% | |
| 2 | human | 5368 | PNOC | prepronociceptin | XM_005273532.2 | 87% | 86.5% | 522_526delCGTCCinsTA;528G>T;532_603del |
| 3 | human | 5368 | PNOC | prepronociceptin | XM_011544559.2 | 87% | 86.5% | 522_526delCGTCCinsTA;528G>T;532_603del |
| 4 | human | 5368 | PNOC | prepronociceptin | NM_001284244.2 | 63.6% | 63.6% | 0_1ins192 |
| 5 | human | 5368 | PNOC | prepronociceptin | XM_017013578.1 | 55.2% | 54.7% | (many diffs) |
| 6 | mouse | 18155 | Pnoc | prepronociceptin | NM_010932.2 | 81.2% | 78.6% | (many diffs) |
| 7 | mouse | 18155 | Pnoc | prepronociceptin | NM_001205075.1 | 71.3% | 68.3% | (many diffs) |
| 8 | mouse | 18155 | Pnoc | prepronociceptin | XM_006518684.2 | 71.3% | 68.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 594
- ORF length:
- 528
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa agtcctgctt tgtgacctgc tgctgctcag tctcttctcc agtgtgttca 121 gcagttgtca gagggactgt ctcacatgcc aggagaagct ccacccagcc ctggacagct 181 tcgacctgga ggtgtgcatc ctcgagtgtg aagagaaggt cttccccagc cccctctgga 241 ctccatgcac caaggtcatg gcCAGGAGCT CTTGGCAGCT CAGCCCTGCC GCCCCAGAGC 301 ATGTGGCGGC TGCTCTCTAC CAGCCGAGAG CTTCGGAGAT GCAGCATCTG CGGCGAATGC 361 CCCGAGTCCG GAGCTTGTTC CAGGAGCAGG AAGAGCCCGA GCCTGGCATG GAGGAGGCTG 421 GTGAGATGGA GCAGAAGCAG CTGCAGAAGA GATTTGGGGG CTTCACCGGG GCCCGGAAGT 481 CGGCCAGGAA GTTGGCCAAT CAGAAGCGGT TCAGTGAGTT TATGAGGCAA TACTTGGTCC 541 TGAGCATGCA GTCCAGCCAG CGCCGGCGCA CCCTGCACCA GAATGGTAAT GTGTACCCAA 601 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 661 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 721 TATCTTGTGG AAAGGACGAG GACCCTTACT TCTTAAAGTA AATACGCGTT AAGTCgacaa 781 tcaacctctg gattacaaaa tttgtgaaag att