Construct: ORF TRCN0000468696
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006983.1_s317c1
- Derived from:
- ccsbBroadEn_01713
- DNA Barcode:
- TTCATTGTTAAATCCATGTTCTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRPC1 (7220)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468696
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7220 | TRPC1 | transient receptor potentia... | NM_003304.4 | 100% | 100% | |
| 2 | human | 7220 | TRPC1 | transient receptor potentia... | NM_001251845.2 | 95.7% | 95.7% | 328_429del |
| 3 | human | 7220 | TRPC1 | transient receptor potentia... | XM_005247738.4 | 89.5% | 85.4% | (many diffs) |
| 4 | human | 7220 | TRPC1 | transient receptor potentia... | XM_017007121.2 | 88% | 88% | 0_1ins183;145_246del |
| 5 | human | 7220 | TRPC1 | transient receptor potentia... | XM_005247739.2 | 87% | 85.3% | 0_1ins139;32_33ins155 |
| 6 | human | 7220 | TRPC1 | transient receptor potentia... | XR_001740246.1 | 62.5% | (many diffs) | |
| 7 | human | 7220 | TRPC1 | transient receptor potentia... | XR_241506.4 | 48.7% | (many diffs) | |
| 8 | mouse | 22063 | Trpc1 | transient receptor potentia... | NM_001311123.1 | 88.9% | 97.6% | (many diffs) |
| 9 | mouse | 22063 | Trpc1 | transient receptor potentia... | NM_011643.3 | 85.2% | 93.5% | (many diffs) |
| 10 | mouse | 22063 | Trpc1 | transient receptor potentia... | XM_006511053.3 | 84.4% | 92.7% | (many diffs) |
| 11 | mouse | 22063 | Trpc1 | transient receptor potentia... | XM_006511055.3 | 80.9% | 85.4% | (many diffs) |
| 12 | mouse | 22063 | Trpc1 | transient receptor potentia... | XM_006511056.3 | 78.5% | 85.3% | (many diffs) |
| 13 | mouse | 22063 | Trpc1 | transient receptor potentia... | XM_006511057.2 | 76.8% | 85.3% | (many diffs) |
| 14 | mouse | 22063 | Trpc1 | transient receptor potentia... | XM_011242717.2 | 46% | 49% | (many diffs) |
| 15 | mouse | 22063 | Trpc1 | transient receptor potentia... | XR_870756.2 | 38.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2346
- ORF length:
- 2277
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatggcggcc ctgtacccga gcacggacct ctcgggcgcc tcctcctcct 121 ccctgccttc ctctccatcc tcttcctcgc cgaacgaggt gatggcgctg aaggatgtgc 181 gggaggtgaa ggaggagaat acgctgaatg agaagctttt cttgctggcg tgcgacaagg 241 gtgactatta tatggttaaa aagattttgg aggaaaacag ttcaggtgac ttgaacataa 301 attgcgtaga tgtgcttggg agaaatgctg ttaccataac tattgaaaac gaaaacttgg 361 atatactgca gcttcttttg gactacggtt gtcagaaact aatggaacga attcagaatc 421 ctgagtattc aacaactatg gatgttgcac ctgtcatttt agctgctcat cgtaacaact 481 atgaaattct tacaatgctc ttaaaacagg atgtatctct acccaagccc catgcagttg 541 gctgtgaatg cacattgtgt tctgcaaaaa acaaaaagga tagcctccgg cattccaggt 601 ttcgtcttga tatatatcga tgtttggcca gtccagctct aataatgtta acagaggagg 661 atccaattct gagagcattt gaacttagtg ctgatttaaa agaactaagt cttgtggagg 721 tggaattcag gaatgattat gaggaactag cccggcaatg taaaatgttt gctaaggatt 781 tacttgcaca agcccggaat tctcgtgaat tggaagttat tctaaaccat acgtctagtg 841 acgagcctct tgacaaacgg ggattattag aagaaagaat gaatttaagt cgtctaaaac 901 ttgctatcaa atataaccag aaagagtttg tctcccagtc taactgccag cagttcctga 961 acactgtttg gtttggacag atgtcgggtt accgacgcaa gcccacctgt aagaagataa 1021 tgactgtttt gacagtaggc atcttttggc cagttttgtc actttgttat ttgatagctc 1081 ccaaatctca gtttggcaga atcattcaca caccttttat gaaatttatc attcatggag 1141 catcatattt cacatttctg ctgttgctta atctatactc tcttgtctac aatgaggata 1201 agaaaaacac aatggggcca gcccttgaaa gaatagacta tcttcttatt ctgtggatta 1261 ttgggatgat ttggtcagac attaaaagac tctggtatga agggttggaa gactttttag 1321 aagaatctcg taatcaactc agttttgtca tgaattctct ttatttggca acctttgccc 1381 tcaaagtggt tgctcacaac aagtttcatg attttgctga tcggaaggat tgggatgcat 1441 tccatcctac actggtggca gaagggcttt ttgcatttgc aaatgttcta agttatcttc 1501 gtctcttttt tatgtataca accagctcta tcttgggtcc attacagatt tcaatgggac 1561 agatgttaca agattttgga aaatttcttg ggatgtttct tcttgttttg ttttctttca 1621 caattggact gacacaactg tatgataaag gatatacttc aaaggagcag aaggactgtg 1681 taggcatctt ctgtgaacag caaagcaatg ataccttcca ttcgttcatt ggcacctgct 1741 ttgctttgtt ctggtatatt ttctccttag cgcatgtggc aatctttgtc acaagattta 1801 gctatggaga agaactgcag tcctttgtgg gagctgtcat tgttggtaca tacaatgtcg 1861 tggttgtgat tgtgcttacc aaactgctgg tggcaatgct tcataaaagc tttcagttga 1921 tagcaaaTCA TGAAGACAAA GAATGGAAGT TTGCTCGAGC AAAATTATGG CTTAGCTACT 1981 TTGATGACAA ATGTACGTTA CCTCCACCTT TCAACATCAT TCCCTCACCA AAGACTATCT 2041 GCTATATGAT TAGTAGCCTC AGTAAGTGGA TTTGCTCTCA TACATCAAAA GGCAAGGTCA 2101 AACGGCAAAA CAGTTTAAAG GAATGGAGGA ATTTGAAACA GAAGAGAGAT GAAAACTATC 2161 AAAAAGTGAT GTGCTGCCTA GTGCATCGTT ACTTGACTTC CATGAGACAG AAGATGCAAA 2221 GTACAGATCA GGCAACTGTG GAAAATCTAA ACGAACTGCG CCAAGATCTG TCAAAATTCC 2281 GAAATGAAAT AAGGGATTTA CTTGGCTTTC GGACTTCTAA ATATGCTATG TTTTATCCAA 2341 GAAATTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2401 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2461 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTCATTGTT AAATCCATGT TCTCCACGCG 2521 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt