Construct: ORF TRCN0000468701
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003951.1_s317c1
- Derived from:
- ccsbBroadEn_02767
- DNA Barcode:
- ATATGACCATCACTCATCACCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RHOQ (23433)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468701
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23433 | RHOQ | ras homolog family member Q | NM_012249.4 | 100% | 100% | |
| 2 | human | 23433 | RHOQ | ras homolog family member Q | XM_011532726.2 | 86.3% | 78% | (many diffs) |
| 3 | human | 23433 | RHOQ | ras homolog family member Q | XM_017003716.1 | 80.3% | 78% | (many diffs) |
| 4 | human | 23433 | RHOQ | ras homolog family member Q | XM_011532728.2 | 76.5% | 68.7% | 0_1ins85;54_55ins59 |
| 5 | human | 23433 | RHOQ | ras homolog family member Q | XM_005264229.2 | 57.5% | 57.5% | 199_200ins261 |
| 6 | mouse | 104215 | Rhoq | ras homolog family member Q | NM_145491.2 | 94.1% | 99.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 684
- ORF length:
- 615
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctcacggg cccggcgcgc tgatgctcaa gtgcgtggtg gtcggcgacg 121 gggcggtggg caagacgtgc ctactcatga gctatgccaa cgacgccttc ccggaggagt 181 acgtgcccac cgtcttcgac cactacgcag tcagcgtcac cgtggggggc aagcagtacc 241 tcctaggact ctatgacacg gccggacagg aaGACTATGA CCGTCTGAGG CCTTTATCTT 301 ACCCAATGAC CGATGTCTTC CTTATATGCT TCTCGGTGGT AAATCCAGCC TCATTTCAAA 361 ATGTGAAAGA GGAGTGGGTA CCGGAACTTA AGGAATACGC ACCAAATGTA CCCTTTTTAT 421 TAATAGGAAC TCAGATTGAT CTCCGAGATG ACCCCAAAAC TTTAGCAAGA CTGAATGATA 481 TGAAAGAAAA ACCTATATGT GTGGAACAAG GACAGAAACT AGCAAAAGAG ATAGGAGCAT 541 GCTGCTATGT GGAATGTTCA GCTTTAACCC AGAAGGGATT GAAGACTGTT TTTGATGAGG 601 CTATCATAGC CATTTTAACT CCAAAGAAAC ACACTGTAAA AAAAAGAATA GGATCAAGAT 661 GTATAAACTG TTGTTTAATT ACGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 721 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 781 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TATGACCATC 841 ACTCATCACC CGGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 901 att