Construct: ORF TRCN0000468715
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009463.1_s317c1
- Derived from:
- ccsbBroadEn_10205
- DNA Barcode:
- CCGGTCATATTCAGCACCATGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LGALS8 (3964)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468715
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3964 | LGALS8 | galectin 8 | NM_201543.3 | 99.6% | 99.6% | 1_3delATG |
| 2 | human | 3964 | LGALS8 | galectin 8 | NM_201544.3 | 99.6% | 99.6% | 1_3delATG |
| 3 | human | 3964 | LGALS8 | galectin 8 | NM_006499.4 | 88% | 88% | 1_3delATG;550_675del |
| 4 | human | 3964 | LGALS8 | galectin 8 | NM_201545.2 | 88% | 88% | 1_3delATG;550_675del |
| 5 | human | 3964 | LGALS8 | galectin 8 | XM_017001274.2 | 87.9% | 85.4% | (many diffs) |
| 6 | human | 3964 | LGALS8 | galectin 8 | XM_011544188.3 | 77.7% | 75.4% | (many diffs) |
| 7 | human | 3964 | LGALS8 | galectin 8 | XM_017001275.2 | 61.8% | 59.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1014
- ORF length:
- 948
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt gtccttaaac aacctacaga atatcatcta taacccggta atcccgtttg 121 ttggcaccat tcctgatcag ctggatcctg gaactttgat tgtgatacgt gggcatgttc 181 ctagtgacgc agacagattc caggtggatc tgcagaatgg cagcagcatg aaacctcgag 241 ccgatgtggc ctttcatttc aatcctcgtt tcaaaagggc cggctgcatt gtttgcaata 301 ctttgataaa tgaaaaatgg ggacgggaag agatcaccta tgacacgcct ttcaaaagag 361 aaaagtcttt tgagatcgtg attatggtgc tgaaggacaa attccaggtg gctgtaaatg 421 gaaaacatac tctgctctat ggccacagga tcggcccaga gaaaatagac actctgggca 481 tttatggcaa agtgaatatt cactcaattg gttttagctt cagctcggac ttacaaagta 541 cccaagcatc tagtctggaa ctgacagaga taagtagaga aaatgttcca aagtctggca 601 cgccccagct taggctgcca ttcgctgcaa ggttgaacac ccccatgggc cctggacgaa 661 ctGTCGTCGT TAAAGGAGAA GTGAATGCAA ATGCCAAAAG CTTTAATGTT GACCTACTAG 721 CAGGAAAATC AAAGGATATT GCTCTACACT TGAACCCACG CCTGAATATT AAAGCATTTG 781 TAAGAAATTC TTTTCTTCAG GAGTCCTGGG GAGAAGAAGA GAGAAATATT ACCTCTTTCC 841 CATTTAGTCC TGGGATGTAC TTTGAGATGA TAATTTATTG TGATGTTAGA GAATTCAAGG 901 TTGCAGTAAA TGGCGTACAC AGCCTGGAGT ACAAACACAG ATTTAAAGAG CTCAGCAGTA 961 TTGACACGCT GGAAATTAAT GGAGACATCC ACTTACTGGA AGTAAGGAGC TGGTACCCAA 1021 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1081 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1141 TATCTTGTGG AAAGGACGAC CGGTCATATT CAGCACCATG CCTACGCGTT AAGTCgacaa 1201 tcaacctctg gattacaaaa tttgtgaaag att