Transcript: Human NM_201544.3

Homo sapiens galectin 8 (LGALS8), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-06-09
Taxon:
Homo sapiens (human)
Gene:
LGALS8 (3964)
Length:
5948
CDS:
184..1137

Additional Resources:

NCBI RefSeq record:
NM_201544.3
NBCI Gene record:
LGALS8 (3964)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_201544.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000057353 CGCCTGAATATTAAAGCATTT pLKO.1 880 CDS 100% 10.800 15.120 N LGALS8 n/a
2 TRCN0000419213 TATCATCTATAACCCGGTAAT pLKO_005 213 CDS 100% 10.800 8.640 N LGALS8 n/a
3 TRCN0000435504 TATGGAGTACCTACTATAATA pLKO_005 1417 3UTR 100% 15.000 10.500 N LGALS8 n/a
4 TRCN0000419140 GGGTCCTCTGGGATTAGTTAT pLKO_005 1607 3UTR 100% 13.200 9.240 N LGALS8 n/a
5 TRCN0000416178 TATTACCTCTTTCCCATTTAG pLKO_005 948 CDS 100% 13.200 9.240 N LGALS8 n/a
6 TRCN0000433849 TACACAGCCTGGAGTACAAAC pLKO_005 1037 CDS 100% 10.800 7.560 N LGALS8 n/a
7 TRCN0000057354 CCTGGAACTTTGATTGTGATA pLKO.1 268 CDS 100% 4.950 3.465 N LGALS8 n/a
8 TRCN0000057357 GCTGGAAATTAATGGAGACAT pLKO.1 1089 CDS 100% 4.950 3.465 N LGALS8 n/a
9 TRCN0000057355 GCAAAGTGAATATTCACTCAA pLKO.1 608 CDS 100% 0.495 0.347 N LGALS8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_201544.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10205 pDONR223 100% 99.6% 99.6% None 1_3delATG n/a
2 ccsbBroad304_10205 pLX_304 0% 99.6% 99.6% V5 1_3delATG n/a
3 TRCN0000468715 CCGGTCATATTCAGCACCATGCCT pLX_317 37.3% 99.6% 99.6% V5 1_3delATG n/a
4 ccsbBroadEn_10438 pDONR223 100% 87.1% 86.6% None (many diffs) n/a
5 ccsbBroad304_10438 pLX_304 0% 87.1% 86.6% V5 (many diffs) n/a
6 TRCN0000472443 GGTGAGATCTCTGTGTCTTCTTAT pLX_317 46.4% 87.1% 86.6% V5 (many diffs) n/a
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