Construct: ORF TRCN0000468726
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010174.1_s317c1
- Derived from:
- ccsbBroadEn_10023
- DNA Barcode:
- CAAAGAGTATTCCATGTAGACAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYLK4 (340156)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468726
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 340156 | MYLK4 | myosin light chain kinase f... | NM_001012418.5 | 99.9% | 99.7% | 148G>A |
| 2 | human | 340156 | MYLK4 | myosin light chain kinase f... | XM_005249079.2 | 99.9% | 99.7% | 148G>A |
| 3 | human | 340156 | MYLK4 | myosin light chain kinase f... | XM_006715082.3 | 90.5% | 85.1% | (many diffs) |
| 4 | human | 340156 | MYLK4 | myosin light chain kinase f... | NM_001347872.2 | 87.3% | 87.1% | 1_168del;316G>A |
| 5 | human | 340156 | MYLK4 | myosin light chain kinase f... | XM_005249078.4 | 82.6% | 82.5% | 1_243del;391G>A |
| 6 | human | 340156 | MYLK4 | myosin light chain kinase f... | XR_926185.3 | 36.9% | (many diffs) | |
| 7 | mouse | 238564 | Mylk4 | myosin light chain kinase f... | NM_001166030.1 | 83.2% | 84.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1233
- ORF length:
- 1164
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttaaaagtg aagaggctgg aagaattcaa cacgtgttat aacagcaacc 121 agctggagaa aatggccttt tttcagtgca gggaagaggt ggagaaagtg aagtgttttc 181 tggaaaaaaa ttctggggac caggattcaa gatctagaca taatgaggcg aaggaggtgt 241 ggtcaaacgc cgacctgacg gaaaggatgc ccgtcaaaag caaaaggaca tcagccctcg 301 cagttgacat cccggctcct ccggccccat ttgatcatcg tattgtgaca gccaagcaag 361 gagcggtcaa cagcttctat actgtgagca agacagaaat cctaggagga gggcgtttcg 421 gccaggttca caagtgtgag gagacggcca caggtctgaa gctggcagcc aaaatcatca 481 agaccagagg catgaaggac aaggaggagg tgaagaacga gatcagcgtc atgaaccagc 541 tggaccacgc gaacctcatc cagctgtacg atgccttcga gtctaagaac gacattgtcc 601 tggtcatgga gtatgtggat ggtggggagc tgtttgaccg catcatcgat gagagctaca 661 atttgacgga gcttgatacc atcctgttca tgaagcagat atgtgagggg ataaggcaca 721 tgcatcagat gtacattctc cacttggacc tgaagcctga gaatatcctg tgtgtgaatc 781 gggatgctaa gcaaataaaa attattgatt ttggattggc cagaagatac aaacccagag 841 agaagctgaa ggtgaacttt GGAACCCCAG AATTTCTCGC CCCTGAAGTT GTGAACTATG 901 ATTTTGTTTC ATTTCCCACT GACATGTGGA GTGTGGGGGT CATCGCCTAT ATGCTACTTA 961 GCGGTTTGTC GCCTTTCCTG GGTGACAATG ATGCTGAGAC GCTGAACAAC ATCCTGGCCT 1021 GCAGGTGGGA CTTAGAGGAT GAAGAATTTC AGGACATCTC GGAGGAGGCC AAGGAGTTCA 1081 TCTCTAAGCT TCTGATTAAG GAGAAGAGTT GGCGAATAAG TGCAAGCGAA GCTCTCAAGC 1141 ACCCCTGGTT GTCAGACCAC AAGCTCCACT CCAGACTCAA TGCCCAGAAG AAGAAGAATC 1201 GTGGCTCTGA TGCCCAGGAC TTTGTGACCA AATTGCCAAC TTTCTTGTAC AAAGTGGTTG 1261 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1321 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 1381 AAGAGTATTC CATGTAGACA AGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1441 ttgtgaaaga tt