Construct: ORF TRCN0000468726

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010174.1_s317c1
Derived from:
ccsbBroadEn_10023
DNA Barcode:
CAAAGAGTATTCCATGTAGACAAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MYLK4 (340156)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468726
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 340156 MYLK4 myosin light chain kinase f... NM_001012418.5 99.9% 99.7% 148G>A
2 human 340156 MYLK4 myosin light chain kinase f... XM_005249079.2 99.9% 99.7% 148G>A
3 human 340156 MYLK4 myosin light chain kinase f... XM_006715082.3 90.5% 85.1% (many diffs)
4 human 340156 MYLK4 myosin light chain kinase f... NM_001347872.2 87.3% 87.1% 1_168del;316G>A
5 human 340156 MYLK4 myosin light chain kinase f... XM_005249078.4 82.6% 82.5% 1_243del;391G>A
6 human 340156 MYLK4 myosin light chain kinase f... XR_926185.3 36.9% (many diffs)
7 mouse 238564 Mylk4 myosin light chain kinase f... NM_001166030.1 83.2% 84.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1233
ORF length:
1164
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gttaaaagtg aagaggctgg aagaattcaa cacgtgttat aacagcaacc
121agctggagaa aatggccttt tttcagtgca gggaagaggt ggagaaagtg aagtgttttc
181tggaaaaaaa ttctggggac caggattcaa gatctagaca taatgaggcg aaggaggtgt
241ggtcaaacgc cgacctgacg gaaaggatgc ccgtcaaaag caaaaggaca tcagccctcg
301cagttgacat cccggctcct ccggccccat ttgatcatcg tattgtgaca gccaagcaag
361gagcggtcaa cagcttctat actgtgagca agacagaaat cctaggagga gggcgtttcg
421gccaggttca caagtgtgag gagacggcca caggtctgaa gctggcagcc aaaatcatca
481agaccagagg catgaaggac aaggaggagg tgaagaacga gatcagcgtc atgaaccagc
541tggaccacgc gaacctcatc cagctgtacg atgccttcga gtctaagaac gacattgtcc
601tggtcatgga gtatgtggat ggtggggagc tgtttgaccg catcatcgat gagagctaca
661atttgacgga gcttgatacc atcctgttca tgaagcagat atgtgagggg ataaggcaca
721tgcatcagat gtacattctc cacttggacc tgaagcctga gaatatcctg tgtgtgaatc
781gggatgctaa gcaaataaaa attattgatt ttggattggc cagaagatac aaacccagag
841agaagctgaa ggtgaacttt GGAACCCCAG AATTTCTCGC CCCTGAAGTT GTGAACTATG
901ATTTTGTTTC ATTTCCCACT GACATGTGGA GTGTGGGGGT CATCGCCTAT ATGCTACTTA
961GCGGTTTGTC GCCTTTCCTG GGTGACAATG ATGCTGAGAC GCTGAACAAC ATCCTGGCCT
1021GCAGGTGGGA CTTAGAGGAT GAAGAATTTC AGGACATCTC GGAGGAGGCC AAGGAGTTCA
1081TCTCTAAGCT TCTGATTAAG GAGAAGAGTT GGCGAATAAG TGCAAGCGAA GCTCTCAAGC
1141ACCCCTGGTT GTCAGACCAC AAGCTCCACT CCAGACTCAA TGCCCAGAAG AAGAAGAATC
1201GTGGCTCTGA TGCCCAGGAC TTTGTGACCA AATTGCCAAC TTTCTTGTAC AAAGTGGTTG
1261ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1321GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA
1381AAGAGTATTC CATGTAGACA AGACGCGTTA AGTCgacaat caacctctgg attacaaaat
1441ttgtgaaaga tt

Download FASTA (ORF) (Full)