Construct: ORF TRCN0000468731
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002472.1_s317c1
- Derived from:
- ccsbBroadEn_01924
- DNA Barcode:
- TGCGAACAGACTGACTAACACAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BCAR3 (8412)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468731
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8412 | BCAR3 | BCAR3 adaptor protein, NSP ... | NM_001261408.2 | 100% | 100% | |
| 2 | human | 8412 | BCAR3 | BCAR3 adaptor protein, NSP ... | NM_001261409.1 | 100% | 100% | |
| 3 | human | 8412 | BCAR3 | BCAR3 adaptor protein, NSP ... | NM_003567.4 | 100% | 100% | |
| 4 | human | 8412 | BCAR3 | BCAR3 adaptor protein, NSP ... | XM_017002480.1 | 100% | 100% | |
| 5 | human | 8412 | BCAR3 | BCAR3 adaptor protein, NSP ... | NM_001261410.2 | 88.2% | 85.3% | (many diffs) |
| 6 | human | 8412 | BCAR3 | BCAR3 adaptor protein, NSP ... | NM_001308251.1 | 60.7% | 60.7% | 0_1ins972 |
| 7 | mouse | 29815 | Bcar3 | breast cancer anti-estrogen... | NM_013867.2 | 84.3% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2541
- ORF length:
- 2475
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcaggaaaa tttgcaagcc ttcccagaaa catgccggtg aatcaccagt 121 tccccctggc ctcatccatg gaccttctga gcagcaggtc ccctctcgct gagcatcgcc 181 cagatgccta tcaagatgtg tctatacatg gcacccttcc acggaagaaa aaaggtcctc 241 ctcccataag gtcctgtgat gacttcagtc acatgggcac cctcccccac tccaaatccc 301 cacggcagaa ctcgcctgtg acccaggatg gcatccagga gagcccatgg caggaccggc 361 acggcgaaac cttcaccttc agggatccac atcttctgga cccaactgtg gaatatgtga 421 agttctccaa ggagaggcac atcatggaca ggacccccga gaaactgaag aaggagctgg 481 aggaggagct gctcctgagc agcgaggacc tgcgcagcca tgcctggtac cacggccgca 541 tcccccgaca ggtgtctgaa aaccttgtgc agcgagatgg tgacttccta gttcgtgact 601 ctctgtccag ccctgggaac tttgtcctga cctgtcagtg gaagaacctc gctcagcact 661 tcaaaatcaa ccggacagtt ctgcgactca gcgaggccta cagccgcgtg cagtaccagt 721 tcgagatgga gagcttcgac tccatccccg gcctggtgcg ctgctacgtg ggcaaccgcc 781 ggcccatctc ccagcagagt ggcgccatca tcttccagcc catcaacagg acggtgcctc 841 tgcggtgcct ggaggagcat tatggcacct ccccaggcca ggcccgggag ggcagcctca 901 ccaagggaag gccggatgtg gccaagaggc tgagcctcac catgggtggc gtccaggccc 961 gagagcagaa tttgcccagg ggaaacctcc tcagaaacaa agaaaagagt ggtagccagc 1021 ccgcctgcct ggatcacatg caggacagaa gagccttgtc cctcaaagcc caccagtcag 1081 agagctacct gccgattggc tgcaagctgc cacctcagtc ctcgggtgtg gacacaagcc 1141 cctgcccaaa ctcacctgtg ttcaggacgg gaagcgagcc tgccctgagc ccagcagtgg 1201 ttcggagggt ctcctcagac gccagggctg gggaggcgct gaggggatca gacagtcaac 1261 tgtgccctaa gcccccgcct aagccctgca aggtgccgtt cctcaaggtt ccctcgtctc 1321 cctctgcctg gctcaactca gaggccaact actgtgaact gaacccagcg tttgccacag 1381 gctgcggcag gggagcaaag ctaccctcat gtgcccaggg aagccacaca gaactgctca 1441 cagccaagca gaatgaggcg ccaggtcccc ggaactctgg cgtcaactac ttgatccttg 1501 atgatgatga cagggaaaga ccttgggaac ctgcggcagc tcagatggag aaggggcagt 1561 gggacaaggg cgagtttgtg acgcccctcc tggagactgt ctcctccttc aggcccaacg 1621 agtttgagtc aaagttcctt ccccctgaga ataagcccct ggaaacagca atgttgaaac 1681 gtgcaaaaga actgttcacc aacaacgacc ccaaggtcat cgcccagcac gtactgagca 1741 tggactgcag ggttgctagg atacttggag tctctgaaga gatgaggagg aacatggggg 1801 tgagctcagg cctggaactc attaccttgc ctcacggaca ccagctgcgc ctggacataa 1861 ttgaaagaca caacacaatg gccatcggca ttgcagtgga cattctggga tgcacgggca 1921 ctttggagga ccgagcggcc actctgagta agatcatcca ggtggcggtg gaactgaagg 1981 attccatggg ggacctctat tccttctcag ctctcatgaa agccctggaa atgccacaga 2041 tcacaaggtt agaaaagacg tggactgctc tgcggcacca gtacacccaa actgccattc 2101 tctatgagaa acagctgaag cccttcagca aactcctGCA TGAAGGCAGA GAGTCCACAT 2161 GTGTTCCCCC AAACAATGTA TCAGTCCCAC TGCTGATGCC GCTTGTGACG TTAATGGAGC 2221 GCCAGGCTGT GACTTTTGAA GGAACCGACA TGTGGGAAAA AAACGACCAG AGCTGTGAAA 2281 TCATGCTGAA CCATTTGGCA ACAGCGCGAT TCATGGCCGA GGCTGCAGAC AGCTACCGGA 2341 TGAATGCTGA GAGGATCCTG GCAGGTTTTC AACCAGATGA AGAAATGAAT GAAATCTGCA 2401 AGACTGAATT TCAAATGCGA TTGCTATGGG GCAGCAAAGG TGCACAAGTC AATCAGACAG 2461 AGAGATATGA GAAATTCAAC CAGATTTTAA CTGCCCTCTC GCGTAAATTG GAACCTCCTC 2521 CTGTAAAGCA GGCAGAGCTT TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2581 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2641 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGCG AACAGACTGA 2701 CTAACACAGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt