Construct: ORF TRCN0000468731

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002472.1_s317c1
Derived from:
ccsbBroadEn_01924
DNA Barcode:
TGCGAACAGACTGACTAACACAGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BCAR3 (8412)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468731
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8412 BCAR3 BCAR3 adaptor protein, NSP ... NM_001261408.2 100% 100%
2 human 8412 BCAR3 BCAR3 adaptor protein, NSP ... NM_001261409.1 100% 100%
3 human 8412 BCAR3 BCAR3 adaptor protein, NSP ... NM_003567.4 100% 100%
4 human 8412 BCAR3 BCAR3 adaptor protein, NSP ... XM_017002480.1 100% 100%
5 human 8412 BCAR3 BCAR3 adaptor protein, NSP ... NM_001261410.2 88.2% 85.3% (many diffs)
6 human 8412 BCAR3 BCAR3 adaptor protein, NSP ... NM_001308251.1 60.7% 60.7% 0_1ins972
7 mouse 29815 Bcar3 breast cancer anti-estrogen... NM_013867.2 84.3% 85.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2541
ORF length:
2475
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tgcaggaaaa tttgcaagcc ttcccagaaa catgccggtg aatcaccagt
121tccccctggc ctcatccatg gaccttctga gcagcaggtc ccctctcgct gagcatcgcc
181cagatgccta tcaagatgtg tctatacatg gcacccttcc acggaagaaa aaaggtcctc
241ctcccataag gtcctgtgat gacttcagtc acatgggcac cctcccccac tccaaatccc
301cacggcagaa ctcgcctgtg acccaggatg gcatccagga gagcccatgg caggaccggc
361acggcgaaac cttcaccttc agggatccac atcttctgga cccaactgtg gaatatgtga
421agttctccaa ggagaggcac atcatggaca ggacccccga gaaactgaag aaggagctgg
481aggaggagct gctcctgagc agcgaggacc tgcgcagcca tgcctggtac cacggccgca
541tcccccgaca ggtgtctgaa aaccttgtgc agcgagatgg tgacttccta gttcgtgact
601ctctgtccag ccctgggaac tttgtcctga cctgtcagtg gaagaacctc gctcagcact
661tcaaaatcaa ccggacagtt ctgcgactca gcgaggccta cagccgcgtg cagtaccagt
721tcgagatgga gagcttcgac tccatccccg gcctggtgcg ctgctacgtg ggcaaccgcc
781ggcccatctc ccagcagagt ggcgccatca tcttccagcc catcaacagg acggtgcctc
841tgcggtgcct ggaggagcat tatggcacct ccccaggcca ggcccgggag ggcagcctca
901ccaagggaag gccggatgtg gccaagaggc tgagcctcac catgggtggc gtccaggccc
961gagagcagaa tttgcccagg ggaaacctcc tcagaaacaa agaaaagagt ggtagccagc
1021ccgcctgcct ggatcacatg caggacagaa gagccttgtc cctcaaagcc caccagtcag
1081agagctacct gccgattggc tgcaagctgc cacctcagtc ctcgggtgtg gacacaagcc
1141cctgcccaaa ctcacctgtg ttcaggacgg gaagcgagcc tgccctgagc ccagcagtgg
1201ttcggagggt ctcctcagac gccagggctg gggaggcgct gaggggatca gacagtcaac
1261tgtgccctaa gcccccgcct aagccctgca aggtgccgtt cctcaaggtt ccctcgtctc
1321cctctgcctg gctcaactca gaggccaact actgtgaact gaacccagcg tttgccacag
1381gctgcggcag gggagcaaag ctaccctcat gtgcccaggg aagccacaca gaactgctca
1441cagccaagca gaatgaggcg ccaggtcccc ggaactctgg cgtcaactac ttgatccttg
1501atgatgatga cagggaaaga ccttgggaac ctgcggcagc tcagatggag aaggggcagt
1561gggacaaggg cgagtttgtg acgcccctcc tggagactgt ctcctccttc aggcccaacg
1621agtttgagtc aaagttcctt ccccctgaga ataagcccct ggaaacagca atgttgaaac
1681gtgcaaaaga actgttcacc aacaacgacc ccaaggtcat cgcccagcac gtactgagca
1741tggactgcag ggttgctagg atacttggag tctctgaaga gatgaggagg aacatggggg
1801tgagctcagg cctggaactc attaccttgc ctcacggaca ccagctgcgc ctggacataa
1861ttgaaagaca caacacaatg gccatcggca ttgcagtgga cattctggga tgcacgggca
1921ctttggagga ccgagcggcc actctgagta agatcatcca ggtggcggtg gaactgaagg
1981attccatggg ggacctctat tccttctcag ctctcatgaa agccctggaa atgccacaga
2041tcacaaggtt agaaaagacg tggactgctc tgcggcacca gtacacccaa actgccattc
2101tctatgagaa acagctgaag cccttcagca aactcctGCA TGAAGGCAGA GAGTCCACAT
2161GTGTTCCCCC AAACAATGTA TCAGTCCCAC TGCTGATGCC GCTTGTGACG TTAATGGAGC
2221GCCAGGCTGT GACTTTTGAA GGAACCGACA TGTGGGAAAA AAACGACCAG AGCTGTGAAA
2281TCATGCTGAA CCATTTGGCA ACAGCGCGAT TCATGGCCGA GGCTGCAGAC AGCTACCGGA
2341TGAATGCTGA GAGGATCCTG GCAGGTTTTC AACCAGATGA AGAAATGAAT GAAATCTGCA
2401AGACTGAATT TCAAATGCGA TTGCTATGGG GCAGCAAAGG TGCACAAGTC AATCAGACAG
2461AGAGATATGA GAAATTCAAC CAGATTTTAA CTGCCCTCTC GCGTAAATTG GAACCTCCTC
2521CTGTAAAGCA GGCAGAGCTT TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
2581CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
2641AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGCG AACAGACTGA
2701CTAACACAGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)