Construct: ORF TRCN0000468756
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004002.1_s317c1
- Derived from:
- ccsbBroadEn_08755
- DNA Barcode:
- TCAAGCCAGCCGACGTACGTGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BEND3 (57673)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468756
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57673 | BEND3 | BEN domain containing 3 | NM_001080450.2 | 99.9% | 99.8% | 1787A>G |
| 2 | human | 57673 | BEND3 | BEN domain containing 3 | NM_001367314.1 | 99.9% | 99.8% | 1787A>G |
| 3 | human | 57673 | BEND3 | BEN domain containing 3 | XM_005267080.4 | 99.9% | 99.8% | 1787A>G |
| 4 | human | 57673 | BEND3 | BEN domain containing 3 | XM_011536005.3 | 99.9% | 99.8% | 1787A>G |
| 5 | human | 650623 | BEND3P3 | BEN domain containing 3 pse... | NR_027512.1 | 34.8% | (many diffs) | |
| 6 | mouse | 331623 | Bend3 | BEN domain containing 3 | NM_199028.2 | 86.1% | 90.7% | (many diffs) |
| 7 | mouse | 331623 | Bend3 | BEN domain containing 3 | XM_006512769.3 | 86.1% | 90.7% | (many diffs) |
| 8 | mouse | 331623 | Bend3 | BEN domain containing 3 | XM_006512770.3 | 86.1% | 90.7% | (many diffs) |
| 9 | mouse | 331623 | Bend3 | BEN domain containing 3 | XM_006512771.3 | 86.1% | 90.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2553
- ORF length:
- 2484
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaactcaact gaattcaccg aagatgtaga agaagttcta aaaagtatca 121 ctgtgaaagt ggagacagag gctgaagatg ctgctctgga ctgctccgtg aattccagga 181 cttctgagaa gcactctgtg gacagcgtcc tcactgccct gcaggactcc agcaaacgaa 241 agcagctggt cagcgatggc ctgctagact ctgtccccgg cgtgaagagg aggcggctga 301 tccccgaggc tctcctagca ggcatgcgga accgtgagaa cagctcgccc tgccaaggca 361 atggtgagca ggccggcagg ggcaggagcc tgggcaatgt gtggcctgga gaggaggagc 421 cctgcaacga tgccaccacc ccttcctaca agaagcctct gtatggcatc tcgcacaaga 481 tcatggagaa gaagaatcct ccctcggggg acctgctaaa cgtgtacgag ctctttgaga 541 aggcaaacgc cagcaacagc ccctcgtcac tgcggctcct gaatgagcca cagaagcggg 601 actgtggcag caccggggca ggcactgaca acgaccccaa catctacttc ctgatccaga 661 agatgttcta catgctgaac accctcacgt ccaacatgtc ccagctgcac agcaaggtgg 721 acctgctctc ccttgaggtg agccgcatca agaagcaggt gagccccact gagatggtgg 781 ccaaattcca gccgccccct gagtaccagc tcacagccgc agagctcaag cagatcgtgg 841 accagagcct gtcagggggg gacctggcct gccgcttgct ggtgcagctc ttccccgagc 901 tcttcagcga cgtggacttc tcccggggct gcagtgcctg tggctttgcg gccaagcgca 961 agctggagtc gctgcacctg cagctcatcc gcaactatgt ggaggtctac tacccctcgg 1021 tgaaggacac ggctgtgtgg caggccgagt gcctgcccca gctgaacgac ttcttcagcc 1081 gcttctgggc ccagcgggaa atggaggaca gccagcccag cggccaggtc gccagcttct 1141 ttgaggcaga gcaggtggac cccggccact tcctggacaa caaagaccag gaggaggccc 1201 tgtctcttga ccggagcagc accatcgcct cagaccacgt ggtggacacg caggacctca 1261 ctgagttcct ggacgaagcc tcctcaccag gcgagtttgc cgtcttcctc ctccaccggc 1321 tcttccccga gctcttcgac caccgcaagc tgggtgaaca gtacagctgc tacggggacg 1381 gtggaaagca ggagctggac ccgcagcggc tgcagatcat ccgcaactac acggagatct 1441 acttccctga catgcaggag gaggaggcct ggctgcagca gtgtgcccag cgcatcaacg 1501 acgagctcga gggcctgggg ctggacgcgg gcagtgaagg cgaccccccg cgtgatgact 1561 gctacgactc ctccagtctg cccgacgaca tctcagtggt caaggtggag gacagcttcg 1621 agggcgagcg gccgggtcgc cgctccaaga agatctggct ggtgcccatc gacttcgaca 1681 agttagagat cccccagcct gacttcgagg tgcccggtgc cgactgcctg ctcagcaagg 1741 agcagctacg cagcatctac gagagcagcc tgtccatcgg caacttcgcc tcgcgcctgc 1801 tggtgcacct gttccccgag ctcttcacgc acgagaacct gcgcaagcag tacagctgca 1861 gcggctccct gggcaagaag cagctggacc cctcccgcat caagctcatc cgccactacg 1921 tgcagctgct ctacccacgc gccaaaaacg accgcgtctg gaccctggag ttcgtgggca 1981 aactggatga gcgctgccgg cgccgggaca cggagcaaag gcgctcctac cagcagcagc 2041 gcaaggtcca cgtgccgggc cctgagtgca gagacttgac cagctatgca atcaaccccg 2101 agaggttccg ggaggagttt gaggggcccc cactgccccc cgagaggagc agcaaggact 2161 tttgcaagat ccccttggac gagctggtgg tcccctcgcc tgacttcccg gtgccttctc 2221 cctacctgct gtctgacaag gaggtgcgtg agatcgtgca gcagagcctc tccgtgggca 2281 actttgccgc ccggctcctc gtccgcctgt ttcccgaact cttcaccgcc gagaacctcc 2341 ggctgcagta caaccattcc ggggcttgca acaagaagca actggacccc acgcggctgc 2401 ggctcatccg ccacTACGTG GAAGCCGTCT ACCCGGTGGA GAAGATGGAG GAGGTGTGGC 2461 ACTACGAATG TATCCCCAGC ATCGATGAGA GGTGCCGCCG CCCCAACAGG AAAAAATGCG 2521 ACATCCTCAA GAAAGCAAAG AAAGTGGAGA AGTTGCCAAC TTTCTTGTAC AAAGTGGTTG 2581 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2641 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 2701 AAGCCAGCCG ACGTACGTGC CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 2761 ttgtgaaaga tt