Construct: ORF TRCN0000468768
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015581.1_s317c1
- Derived from:
- ccsbBroadEn_12234
- DNA Barcode:
- ACGACCCCGTTCCCTTAATACACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF16B (55614)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468768
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55614 | KIF16B | kinesin family member 16B | NM_001199865.2 | 52.5% | 52.5% | 1996_3798del |
| 2 | human | 55614 | KIF16B | kinesin family member 16B | XM_017027926.1 | 52.1% | 52.1% | 1996_3828del |
| 3 | human | 55614 | KIF16B | kinesin family member 16B | XM_005260755.3 | 52% | 52% | 1784_1816del;2029_3831del |
| 4 | human | 55614 | KIF16B | kinesin family member 16B | XM_006723588.3 | 51.6% | 51.6% | 1784_1816del;2029_3861del |
| 5 | human | 55614 | KIF16B | kinesin family member 16B | XM_005260754.3 | 51.2% | 51.2% | 1784_1816del;2029_3894del |
| 6 | human | 55614 | KIF16B | kinesin family member 16B | NM_024704.5 | 50.4% | 50.4% | 1996_3951del |
| 7 | human | 55614 | KIF16B | kinesin family member 16B | XM_005260753.3 | 50% | 50% | 1784_1816del;2029_3984del |
| 8 | human | 55614 | KIF16B | kinesin family member 16B | NM_001199866.2 | 47.7% | 47.7% | 1996_4176del |
| 9 | human | 55614 | KIF16B | kinesin family member 16B | XM_005260752.3 | 36.2% | 36.2% | 1784_1816del;2029_5496del |
| 10 | human | 55614 | KIF16B | kinesin family member 16B | XM_005260751.4 | 35.5% | 35.5% | 1996_5616del |
| 11 | human | 55614 | KIF16B | kinesin family member 16B | XM_005260750.3 | 35.3% | 35.3% | 1784_1816del;2029_5649del |
| 12 | mouse | 16558 | Kif16b | kinesin family member 16B | XM_006498819.3 | 47.8% | 51.5% | (many diffs) |
| 13 | mouse | 16558 | Kif16b | kinesin family member 16B | XM_006498818.3 | 47% | 50.7% | (many diffs) |
| 14 | mouse | 16558 | Kif16b | kinesin family member 16B | NM_001081133.2 | 46% | 49.5% | (many diffs) |
| 15 | mouse | 16558 | Kif16b | kinesin family member 16B | XM_006498817.3 | 45.6% | 49.1% | (many diffs) |
| 16 | mouse | 16558 | Kif16b | kinesin family member 16B | XM_006498815.3 | 33.1% | 35.6% | (many diffs) |
| 17 | mouse | 16558 | Kif16b | kinesin family member 16B | XM_006498814.2 | 32.4% | 34.8% | (many diffs) |
| 18 | mouse | 16558 | Kif16b | kinesin family member 16B | XM_006498813.3 | 32.2% | 34.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2061
- ORF length:
- 1995
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc atcggtcaag gtggccgtga gggtccggcc catgaatcgc agggaaaagg 121 acttggaggc caagttcatt attcagatgg agaaaagcaa aacgacaatc acaaacttaa 181 agataccaga aggaggcact ggggactcag gaagagaacg gaccaagacc ttcacctatg 241 acttttcttt ttattctgct gatacaaaaa gcccagatta cgtttcacaa gaaatggttt 301 tcaaaaccct cggcacagat gtcgtgaagt ctgcatttga aggttataat gcttgtgtct 361 ttgcatatgg gcaaactgga tctggaaagt catacactat gatgggaaat tctggagatt 421 ctggcttaat acctcggatc tgtgaaggac tcttcagtcg gataaatgaa accaccagat 481 gggatgaagc ttcttttcga actgaagtca gctacttaga aatttataac gaacgtgtga 541 gagatctact tcggcggaag tcatctaaaa ccttcaattt gagagtccgt gagcatccca 601 aagaaggccc ttatgttgag gatttatcca aacatttagt acagaattat ggtgacgtag 661 aagaacttat ggatgcgggc aatatcaacc ggaccaccgc agcgactggg atgaacgacg 721 tcagtagcag gtctcatgcc atcttcacca tcaagttcac tcaggctaaa tttgattctg 781 aaatgccatg tgaaaccgtc agtaagatcc acttggttga tcttgccgga agtgagcgtg 841 cagatgccac cggagccacc ggggttaggc taaaggaagg gggaaatatt aacaagtccc 901 tcgtgactct ggggaacgtc atttctgcct tagctgattt atctcaggat gctgcaaata 961 ctcttgcaaa gaagaagcaa gttttcgtgc cttacaggga ttctgtgttg acttggttgt 1021 taaaagatag ccttggagga aactctaaaa ctatcatgat tgccaccatt tcacctgctg 1081 atgtcaatta tggagaaacc ctaagtactc ttcgctatgc aaatagagcc aaaaacatca 1141 tcaacaagcc taccattaat gaggatgcca acgtcaaact tatccgtgag ctgcgagctg 1201 aaatagccag actgaaaacg ctgcttgctc aagggaatca gattgccctc ttagactccc 1261 ccacagcttt aagtatggag gaaaaacttc agcagaatga agcaagagtt caagaattga 1321 ccaaggaatg gacaaataag tggaatgaaa cccaaaatat tttgaaagaa caaactctag 1381 ccctcaggaa agaagggatt ggagttgttt tggattctga actgcctcat ttgattggca 1441 tcgatgatga ccttttgagt actggaatca tcttatatca tttaaaggaa ggtcagacat 1501 acgttggtag agacgatgct tccacggagc aagatattgt tcttcatggc cttgacttgg 1561 agagtgagca ttgcatcttt gaaaatatcg gggggacagt gactctgata cccctgagtg 1621 ggtcccagtg ctctgtgaat ggtgttcaga tcgtggaggC CACACATCTA AATCAAGGTG 1681 CTGTGATTCT CTTGGGAAGA ACCAATATGT TTCGCTTTAA CCATCCAAAG GAAGCCGCCA 1741 AGCTCAGGGA GAAGAGGAAG AGTGGCCTTC TGTCCTCCTT CAGCTTGTCC ATGACCGACC 1801 TCTCGAAGTC CCGTGAGAAC CTGTCTGCAG TCATGTTGTA TAACCCCGGA CTTGAATTTG 1861 AGAGGCAACA GCGTGAAGAA CTTGAAAAAT TAGAAAGTAA AAGGAAACTC ATAGAAGAAA 1921 TGGAGGAAAA GCAGAAATCA GACAAGGCTG AACTGGAGCG GATGCAGCAG GAGGTGGAGA 1981 CCCAGCGCAA GGAGACAGAA ATCGTGCAGC TCCAGATTCG CAAGCAGGAG GAGAGCCTCA 2041 AACGCCGCAG CTTCCACATC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2101 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2161 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACGA CCCCGTTCCC 2221 TTAATACACA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt