Construct: ORF TRCN0000468772
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002134.1_s317c1
- Derived from:
- ccsbBroadEn_14052
- DNA Barcode:
- GGAAGCTCCTTCTCCACGAGATCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CCT6B (10693)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468772
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10693 | CCT6B | chaperonin containing TCP1 ... | NM_006584.4 | 99.7% | 2.4% | (many diffs) |
| 2 | human | 10693 | CCT6B | chaperonin containing TCP1 ... | NM_001193529.3 | 92.7% | 2.6% | (many diffs) |
| 3 | human | 10693 | CCT6B | chaperonin containing TCP1 ... | NM_001193530.2 | 91.2% | 2.6% | (many diffs) |
| 4 | human | 10693 | CCT6B | chaperonin containing TCP1 ... | XM_017024023.2 | 87.6% | 2.7% | 0_1ins197 |
| 5 | human | 10693 | CCT6B | chaperonin containing TCP1 ... | XM_011524203.3 | 73.2% | 3.3% | 0_1ins425 |
| 6 | human | 10693 | CCT6B | chaperonin containing TCP1 ... | XM_017024024.2 | 66.2% | 3.6% | 0_1ins425;188_189ins111 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 195
- ORF length:
- 129
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcgataagg ccgtcaactc caaggctgag gtggcgcggg cccaggcagc 121 tttggctgtc aatatatgcg ccgcccgagg gctgcaggat gtgctgcgga ccaacttggg 181 tcctaaaggc accatgaaaa tgcttgcttc tggtgcaggt gacatcaaac tcaccaaaga 241 tggcaatgta ctgctcgatg agatgcaaat tcaacatcca acagcttcct tgatagcaaa 301 agtagcaaca gctcaggatg acgtcacagg agatggtact acttcaaatg ttctaattat 361 tggagagtta ttaaaacaag ctgacctgta catttctgag ggcctgcacc ctagaataat 421 agctgaagga tttgaagctg caaagataaa agcacttgaa gttttggagg aagttaaagt 481 gacaaaggag atgaaaagaa aaatcctctt agatgtagct agaacatcat tacaaactaa 541 agttcatgct gaactggctg atgtcttaac agaggttgtg gtggattctg ttttggctgt 601 tagaagacca ggttacccta ttgatctctt catggtagaa ataatggaga tgaagcataa 661 attaggaaca gatacaaagt tgatccaagg attagttttg gatcatggtg cccgtcatcc 721 agatatgaag aagcgagtag aagatgcatt tatccttatt tgcaacgttt cactggaata 781 tgaaaaaaca gaggtgaact ctggtttctt ttataagact gcagaagaga aagagaaatt 841 ggtaaaagct gaaagaaaat ttattgaaga tagagtacaa aaaataatag acctgaagga 901 caaagtctgt gctcagtcaa ataaaggatt tgtcgtcatt aatcaaaagg gaattgatcc 961 attttcctta gattctcttg caaaacatgg aatagtagct cttcgcagag caaaaagaag 1021 aaatatggaa agactctctc ttgcttgtgg tggaatggcc gtgaattctt ttgaagatct 1081 cactgtagat tgcttgggac atgctggtct tgtgtatgag tatacattag gtgaagaaaa 1141 gttcactttt attgaggagt gtgttaaccc ttgctctgtt accttgttGG TTAAAGGACC 1201 AAATAAGCAT ACTCTCACAC AAGTCAAGGA TGCCATAAGA GATGGACTTC GTGCTATCAA 1261 AAATGCCATT GAAGATGGTT GTATGGTTCC TGGAGCTGGT GCAATTGAAG TGGCAATGGC 1321 TGAAGCTCTT GTTACATATA AGAACAGTAT AAAAGGAAGA GCTCGTCTTG GAGTCCAAGC 1381 TTTTGCTGAT GCCTTACTCA TTATTCCCAA GGTTCTTGCT CAGAATGCTG GTTATGACCC 1441 ACAGGAAACA TTAGTAAAAG TTCAGGCTGA GCATGTCGAG TCAAAACAAC TTGTGGGCGT 1501 AGATTTGAAT ACAGGTGAGC CAATGGTAGC AGCAGATGCA GGAGTTTGGG ATAATTATTG 1561 TGTAAAAAAA CAACTTCTTC ACTCTTGCAC AGTGATTGCC ACCAACATTC TCCTGGTTGA 1621 TGAAATTATG CGAGCTGGGA TGTCTTCTCT CAAATGCCCA ACTTTCTTGT ACAAAGTGGT 1681 TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC 1741 TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 1801 GGAAGCTCCT TCTCCACGAG ATCAACGCGT TAAGTCgaca atcaacctct ggattacaaa 1861 atttgtgaaa gatt