Construct: ORF TRCN0000468811
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016133.1_s317c1
- Derived from:
- ccsbBroadEn_09958
- DNA Barcode:
- AATAAAAGCTATTTCATATTATAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZADH2 (284273)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468811
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284273 | ZADH2 | zinc binding alcohol dehydr... | NM_175907.6 | 99.9% | 100% | 225C>T |
| 2 | human | 284273 | ZADH2 | zinc binding alcohol dehydr... | NM_001306093.1 | 67.3% | 67.3% | 0_1ins369 |
| 3 | human | 284273 | ZADH2 | zinc binding alcohol dehydr... | XM_024451166.1 | 67.3% | 67.3% | 0_1ins369 |
| 4 | mouse | 225791 | Zadh2 | zinc binding alcohol dehydr... | NM_146090.5 | 84.5% | 88% | (many diffs) |
| 5 | mouse | 225791 | Zadh2 | zinc binding alcohol dehydr... | XM_006526460.3 | 54.8% | 57.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1197
- ORF length:
- 1131
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gcggctggtg cccaccgggg cccgggccat cgtggacatg tcgtacgccc 121 gccacttcct ggacttccag ggctccgcca ttccccaagc catgcagaag ctggtggtga 181 cccggctgag ccccaacttc cgcgaggccg tcaccctgag ccgggactgc ccggtgccgc 241 tccccgggga cggagacctc ctcgtccgga accgatttgt tggtgttaat gcatctgaca 301 tcaactattc agcaggccgc tatgacccct cagttaagcc tccctttgac ataggtttcg 361 aaggcattgg ggaggtggtg gccctaggcc tctctgctag tgccagatac acagttggcc 421 aagctgtggc ttacatggca cctggttctt ttgctgagta cacagttgtg cctgccagca 481 ttgcaactcc agtgccctca gtgaaacccg agtatcttac cctgctggta agtggcacca 541 ccgcatacat cagcctgaaa gagctcggag gactgtcgga agggaaaaaa gttttggtga 601 cagcagcagc tgggggaacg ggccagtttg ccatgcagct ttcaaagaag gcaaagtgcc 661 atgtaattgg aacctgctct tctgatgaaa agtctgcttt tctgaaatct cttggctgtg 721 atcgtcctat caactataaa actgaacccg taggtaccgt ccttaagcag gagtaccctg 781 aaggtgtcga tgtggtctat gaatctgttg ggggagccat gtttgacttg gctgtagacg 841 ccctGGCTAC GAAAGGGCGC TTGATAGTAA TAGGGTTTAT CTCTGGCTAC CAAACTCCTA 901 CTGGCCTTTC GCCTGTGAAA GCAGGAACAT TGCCAGCCAA ACTGCTCAAG AAATCTGCCA 961 GCGTACAGGG CTTCTTCCTG AACCATTACC TTTCTAAGTA TCAAGCAGCC ATGAGCCACT 1021 TGCTCGAGAT GTGTGTGAGC GGAGACCTGG TTTGTGAGGT GGACCTTGGA GATCTGTCTC 1081 CAGAGGGCAG GTTTACTGGC CTGGAGTCCA TATTCCGTGC TGTCAATTAT ATGTACATGG 1141 GAAAAAACAC TGGAAAAATT GTAGTTGAAT TACCTCACTC TGTCAACAGT AAGCTGTACC 1201 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GAAATAAAAG CTATTTCATA TTATAGACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt