Construct: ORF TRCN0000468816
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008874.1_s317c1
- Derived from:
- ccsbBroadEn_00021
- DNA Barcode:
- AGCCAGTCACCCTGAGGCTAACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACYP1 (97)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468816
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 97 | ACYP1 | acylphosphatase 1 | NM_001107.5 | 100% | 100% | |
| 2 | human | 97 | ACYP1 | acylphosphatase 1 | NM_001302616.2 | 100% | 100% | |
| 3 | human | 97 | ACYP1 | acylphosphatase 1 | NM_001302617.2 | 76.7% | 76.7% | 1_90del |
| 4 | human | 97 | ACYP1 | acylphosphatase 1 | NR_126393.2 | 45% | 1_79del;161_239del;456_659del | |
| 5 | human | 97 | ACYP1 | acylphosphatase 1 | NR_126395.2 | 43.1% | (many diffs) | |
| 6 | human | 97 | ACYP1 | acylphosphatase 1 | NR_126394.2 | 37% | (many diffs) | |
| 7 | mouse | 66204 | Acyp1 | acylphosphatase 1, erythroc... | NM_025421.2 | 85.9% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 363
- ORF length:
- 297
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGC AGAAGGAAAC ACCCTGATAT CAGTGGATTA TGAAATTTTT GGGAAGGTGC 121 AAGGGGTGTT TTTCCGTAAG CATACTCAGG CTGAGGGTAA AAAGCTGGGA TTGGTAGGCT 181 GGGTCCAGAA CACTGACCGG GGCACAGTGC AAGGACAATT GCAAGGTCCC ATCTCCAAGG 241 TGCGTCATAT GCAGGAATGG CTTGAAACAA GAGGAAGTCC TAAATCACAC ATCGACAAAG 301 CAAACTTCAA CAATGAAAAA GTCATCTTGA AGTTGGATTA CTCAGACTTC CAAATTGTAA 361 AATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 421 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 481 GGCTTTATAT ATCTTGTGGA AAGGACGAAG CCAGTCACCC TGAGGCTAAC GCACGCGTTA 541 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt