Transcript: Human NM_001302617.2

Homo sapiens acylphosphatase 1 (ACYP1), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-08-15
Taxon:
Homo sapiens (human)
Gene:
ACYP1 (97)
Length:
790
CDS:
200..589

Additional Resources:

NCBI RefSeq record:
NM_001302617.2
NBCI Gene record:
ACYP1 (97)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001302617.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000353091 CATCTCCAAGGTGCGTCATAT pLKO_005 454 CDS 100% 13.200 9.240 N ACYP1 n/a
2 TRCN0000344801 GGGCACAGTGCAAGGACAATT pLKO_005 424 CDS 100% 13.200 9.240 N ACYP1 n/a
3 TRCN0000050447 AGTCATCTTGAAGTTGGATTA pLKO.1 544 CDS 100% 10.800 7.560 N ACYP1 n/a
4 TRCN0000333277 AGTCATCTTGAAGTTGGATTA pLKO_005 544 CDS 100% 10.800 7.560 N ACYP1 n/a
5 TRCN0000344802 CTAAATCACACATCGACAAAG pLKO_005 504 CDS 100% 10.800 7.560 N ACYP1 n/a
6 TRCN0000344800 ATAAACTCAGTGGTTTGGTTT pLKO_005 614 3UTR 100% 4.950 3.465 N ACYP1 n/a
7 TRCN0000050443 CCTAAATCACACATCGACAAA pLKO.1 503 CDS 100% 4.950 3.465 N ACYP1 n/a
8 TRCN0000050444 CGACAAAGCAAACTTCAACAA pLKO.1 517 CDS 100% 4.950 3.465 N ACYP1 n/a
9 TRCN0000378581 GAGATAGAACTATTGTGTGTT pLKO_005 647 3UTR 100% 4.950 3.465 N ACYP1 n/a
10 TRCN0000378662 GAATGGCTTGAAACAAGAGGA pLKO_005 479 CDS 100% 2.640 1.848 N ACYP1 n/a
11 TRCN0000050446 CCCATCTCCAAGGTGCGTCAT pLKO.1 452 CDS 100% 1.350 0.945 N ACYP1 n/a
12 TRCN0000050445 GATTACTCAGACTTCCAAATT pLKO.1 560 CDS 100% 13.200 7.920 N ACYP1 n/a
13 TRCN0000363131 CATACTCAGGCTGAGGGTAAA pLKO_005 365 CDS 100% 10.800 6.480 N ACYP1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001302617.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00021 pDONR223 100% 76.7% 76.7% None 1_90del n/a
2 ccsbBroad304_00021 pLX_304 0% 76.7% 76.7% V5 1_90del n/a
3 TRCN0000468816 AGCCAGTCACCCTGAGGCTAACGC pLX_317 100% 76.7% 76.7% V5 1_90del n/a
4 TRCN0000488485 GTTCCTGCCATCGTCTCGCAAGAG pLX_317 100% 76.7% 76.7% V5 (not translated due to prior stop codon) 1_90del n/a
5 TRCN0000491344 AATCGTATTCCGTGCACCCGACTG pLX_317 100% 76.5% 76.1% V5 1_90del;387_388insG n/a
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