Construct: ORF TRCN0000468846
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001990.1_s317c1
- Derived from:
- ccsbBroadEn_03733
- DNA Barcode:
- ATCCTCCCCGATCACTCGTGTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR2S2 (56656)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468846
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56656 | OR2S2 | olfactory receptor family 2... | NM_019897.2 | 100% | 100% | |
| 2 | mouse | 29845 | Olfr155 | olfactory receptor 155 | NM_019473.1 | 84.6% | 82.1% | (many diffs) |
| 3 | mouse | 29848 | Olfr29-ps1 | olfactory receptor 29, pseu... | NR_033638.1 | 83.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1026
- ORF length:
- 957
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaaaagcc aatgagacct cccctgtgat ggggttcgtt ctcctgaggc 121 tctctgccca cccagagctg gaaaagacat tcttcgtgct catcctgctg atgtacctcg 181 tgatcctgct gggcaatggg gtcctcatcc tggtgaccat ccttgactcc cgcctgcaca 241 cgcccatgta cttcttccta gggaacctct ccttcctgga catctgcttc actacctcct 301 cagtcccact ggtcctggac agctttttga ctccccagga aaccatctcc ttctcagcct 361 gtgctgtgca gatggcactc tcctttgcca tggcaggaac agagtgcttg ctcctgagca 421 tgatggcatt tgatcgctat gtggccatct gcaaccccct taggtactcc gtgatcatga 481 gcaaggctgc ctacatgccc atggctgcca gctcctgggc tattggtggt gctgcttccg 541 tggtacacac atccttggca attcagctgc ccttctgtgg agacaatgtc atcaaccact 601 tcacctgtga gattctggct gttctaaagt tggcctgtgc tgacatttcc atcaatgtga 661 tcagcatgga ggtgacgaat gtgatcttcc taggagtccc ggttctgttc atctctttct 721 cctatgtctt catcatcacc accatcctga ggatccccTC AGCTGAGGGG AGGAAAAAGG 781 TCTTCTCCAC CTGCTCTGCC CACCTCACCG TGGTGATCGT CTTCTACGGG ACCTTATTCT 841 TCATGTATGG GAAGCCTAAG TCTAAGGACT CCATGGGAGC AGACAAAGAG GATCTTTCAG 901 ACAAACTCAT CCCCCTTTTC TATGGGGTGG TGACCCCGAT GCTCAACCCC ATCATCTATA 961 GCCTGAGGAA CAAGGATGTG AAGGCTGCTG TGAGGAGACT GCTGAGACCA AAAGGCTTCA 1021 CTCAGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1081 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1141 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATCCTCCCC GATCACTCGT GTCTTACGCG 1201 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt