Construct: ORF TRCN0000468870
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006942.1_s317c1
- Derived from:
- ccsbBroadEn_09678
- DNA Barcode:
- AATCTGTCAGAGGCGTTTCTTGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- METTL21A (151194)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468870
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151194 | METTL21A | methyltransferase like 21A | NM_001127395.3 | 99.6% | 99.5% | 471G>A;575C>T |
| 2 | human | 151194 | METTL21A | methyltransferase like 21A | NM_001330130.2 | 99.6% | 99.5% | 471G>A;575C>T |
| 3 | human | 151194 | METTL21A | methyltransferase like 21A | NM_001330134.1 | 99.6% | 99.5% | 471G>A;575C>T |
| 4 | human | 151194 | METTL21A | methyltransferase like 21A | NM_145280.6 | 99.6% | 99.5% | 471G>A;575C>T |
| 5 | human | 151194 | METTL21A | methyltransferase like 21A | XM_005246339.4 | 99.6% | 99.5% | 471G>A;575C>T |
| 6 | human | 151194 | METTL21A | methyltransferase like 21A | XM_005246340.4 | 99.6% | 99.5% | 471G>A;575C>T |
| 7 | human | 151194 | METTL21A | methyltransferase like 21A | XM_024452722.1 | 99.6% | 99.5% | 471G>A;575C>T |
| 8 | human | 151194 | METTL21A | methyltransferase like 21A | XM_024452723.1 | 99.6% | 99.5% | 471G>A;575C>T |
| 9 | human | 151194 | METTL21A | methyltransferase like 21A | NM_001330137.1 | 92% | 91.9% | 259_312del;525G>A;629C>T |
| 10 | human | 151194 | METTL21A | methyltransferase like 21A | XM_005246336.3 | 92% | 91.9% | 259_312del;525G>A;629C>T |
| 11 | human | 151194 | METTL21A | methyltransferase like 21A | XM_005246337.4 | 92% | 91.9% | 259_312del;525G>A;629C>T |
| 12 | human | 151194 | METTL21A | methyltransferase like 21A | XM_006712327.3 | 92% | 91.9% | 259_312del;525G>A;629C>T |
| 13 | human | 151194 | METTL21A | methyltransferase like 21A | XM_024452721.1 | 92% | 91.9% | 259_312del;525G>A;629C>T |
| 14 | human | 151194 | METTL21A | methyltransferase like 21A | XM_011510725.2 | 89.4% | 89.3% | 1_75del;546G>A;650C>T |
| 15 | human | 151194 | METTL21A | methyltransferase like 21A | XM_011510724.2 | 83.2% | 83.1% | (many diffs) |
| 16 | human | 151194 | METTL21A | methyltransferase like 21A | NM_001308021.3 | 54.7% | 42.1% | (many diffs) |
| 17 | human | 151194 | METTL21A | methyltransferase like 21A | NM_001330132.1 | 54.7% | 42.1% | (many diffs) |
| 18 | human | 151194 | METTL21A | methyltransferase like 21A | NM_001330136.1 | 54.7% | 42.1% | (many diffs) |
| 19 | human | 151194 | METTL21A | methyltransferase like 21A | XM_024452724.1 | 54.7% | 42.1% | (many diffs) |
| 20 | human | 151194 | METTL21A | methyltransferase like 21A | XM_024452725.1 | 54.7% | 42.1% | (many diffs) |
| 21 | human | 151194 | METTL21A | methyltransferase like 21A | XM_011510727.3 | 49.1% | 37.9% | (many diffs) |
| 22 | human | 151194 | METTL21A | methyltransferase like 21A | XM_011510728.3 | 49.1% | 37.9% | (many diffs) |
| 23 | human | 151194 | METTL21A | methyltransferase like 21A | XM_024452726.1 | 45.5% | 40.4% | (many diffs) |
| 24 | human | 151194 | METTL21A | methyltransferase like 21A | XM_017003446.1 | 42.4% | 37.5% | (many diffs) |
| 25 | human | 151194 | METTL21A | methyltransferase like 21A | XM_017003447.1 | 42.4% | 37.5% | (many diffs) |
| 26 | human | 151194 | METTL21A | methyltransferase like 21A | NM_001330131.2 | 41.1% | 39.9% | (many diffs) |
| 27 | human | 151194 | METTL21A | methyltransferase like 21A | NM_001330133.1 | 41.1% | 39.9% | (many diffs) |
| 28 | human | 151194 | METTL21A | methyltransferase like 21A | NM_001330135.2 | 41.1% | 39.9% | (many diffs) |
| 29 | human | 151194 | METTL21A | methyltransferase like 21A | XM_011510729.2 | 40.9% | 36.3% | (many diffs) |
| 30 | human | 151194 | METTL21A | methyltransferase like 21A | XM_017003444.1 | 38.1% | 33.8% | (many diffs) |
| 31 | human | 151194 | METTL21A | methyltransferase like 21A | XM_017003445.1 | 36.8% | 35.8% | (many diffs) |
| 32 | mouse | 67099 | Mettl21a | methyltransferase like 21A | NM_025964.3 | 88.8% | 89.4% | (many diffs) |
| 33 | mouse | 67099 | Mettl21a | methyltransferase like 21A | XM_006496203.3 | 88.8% | 89.4% | (many diffs) |
| 34 | mouse | 67099 | Mettl21a | methyltransferase like 21A | XM_006496204.3 | 88.8% | 89.4% | (many diffs) |
| 35 | mouse | 67099 | Mettl21a | methyltransferase like 21A | XR_001785519.1 | 13.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 720
- ORF length:
- 654
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cctcgtgccc tatgaggaga ccacggaatt tgggttgcag aaattccaca 121 agcctcttgc aactttttcc tttgcaaacc acacgatcca gatccggcag gactggagac 181 acctgggagt cgcagcggtg gtttgggatg cggccatcgt tctttccaca tacctggaga 241 tgggagctgt ggagctcagg ggccgcTCTG CCGTGGAGCT GGGTGCTGGC ACGGGGCTGG 301 TGGGCATAGT GGCTGCCCTG CTGGGTGCTC ATGTGACTAT CACGGATCGA AAAGTAGCAT 361 TAGAATTTCT TAAATCAAAC GTTCAAGCCA ACTTACCTCC TCATATCCAA ACTAAAACTG 421 TTGTTAAGGA GCTGACTTGG GGACAAAATT TGGGGAGTTT TTCTCCTGGA GAATTTGACC 481 TGATACTTGG TGCTGATATC ATATATTTAG AAGAAACATT CACAGATCTT CTTCAAACAC 541 TGGAACATCT CTGTAGCAAT CACTCTGTGA TTCTTTTAGC ATGCCGAATT CGCTATGAAC 601 GGGATAACAA CTTCTTAGCA ATGCTGGAGA GGCAATTTAT TGTGAGAAAG GTTCACTACG 661 ATCCTGAAAA AGATGTACAT ATTTACGAAG CACAGAAGAG AAACCAGAAG GAGGACTTAT 721 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 841 TTTATATATC TTGTGGAAAG GACGAAATCT GTCAGAGGCG TTTCTTGGAA CGCGTTAAGT 901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt