Construct: ORF TRCN0000468885
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018295.1_s317c1
- Derived from:
- ccsbBroadEn_10824
- DNA Barcode:
- GTGTAATATTCACATTTTTAGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FLOT2 (2319)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468885
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2319 | FLOT2 | flotillin 2 | NM_004475.3 | 88.7% | 88.1% | (many diffs) |
| 2 | human | 2319 | FLOT2 | flotillin 2 | NM_001330170.2 | 85.8% | 85.8% | (many diffs) |
| 3 | human | 2319 | FLOT2 | flotillin 2 | XM_024450666.1 | 82.4% | 81.8% | (many diffs) |
| 4 | human | 2319 | FLOT2 | flotillin 2 | XM_017024394.1 | 77.3% | 76.7% | (many diffs) |
| 5 | human | 2319 | FLOT2 | flotillin 2 | XM_017024395.1 | 77.3% | 76.7% | (many diffs) |
| 6 | human | 2319 | FLOT2 | flotillin 2 | XM_017024396.1 | 75% | 74.8% | (many diffs) |
| 7 | human | 2319 | FLOT2 | flotillin 2 | XM_005257953.2 | 70.8% | 70.2% | (many diffs) |
| 8 | human | 2319 | FLOT2 | flotillin 2 | XM_024450667.1 | 70.8% | 70.2% | (many diffs) |
| 9 | mouse | 14252 | Flot2 | flotillin 2 | NM_001040403.1 | 81.7% | 87.9% | (many diffs) |
| 10 | mouse | 14252 | Flot2 | flotillin 2 | NM_001284227.1 | 76.7% | 82.3% | (many diffs) |
| 11 | mouse | 14252 | Flot2 | flotillin 2 | NM_001284228.1 | 71.1% | 76.5% | (many diffs) |
| 12 | mouse | 14252 | Flot2 | flotillin 2 | NM_008028.2 | 71.1% | 76.5% | (many diffs) |
| 13 | mouse | 14252 | Flot2 | flotillin 2 | XM_006532192.2 | 71.1% | 76.5% | (many diffs) |
| 14 | mouse | 14252 | Flot2 | flotillin 2 | XM_006532193.3 | 71.1% | 76.5% | (many diffs) |
| 15 | mouse | 14252 | Flot2 | flotillin 2 | XM_006532191.3 | 68.6% | 74.1% | (many diffs) |
| 16 | mouse | 14252 | Flot2 | flotillin 2 | XM_006532194.1 | 64.6% | 70% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1221
- ORF length:
- 1155
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg caattgccac acggtggggc ccaacgaggc gctggtggtt tcagggggct 121 gttgtggttc cgactataaa cagtacgtgt ttggcggctg ggcctgggcc tggtggtgta 181 tctccgacac tcagaggatt tccctagaga ttatgacgtt gcagccccgc tgcgaggacg 241 tagagacggc cgagggggta gctttaactg tgacgggtgt cgcccaggtg aagatcatga 301 cggagaagga actcctggcc gtggcttgtg agcagtttct gggtaagaat gtgcaggaca 361 tcaaaaacgt cgtcctgcag accctggagg gacatctgcg ctccatcctc gggaccctga 421 cagtggagca gatttatcag gaccgggacc agtttgccaa gctggtgcgg gaggtggcag 481 cccctgatgt tggccgcatg ggcattgaga tcctcagctt caccatcaag gacgtgtatg 541 acaaagtgga ctatctgagc tccctgggca agacgcagac tgccgtggtg cagagagatg 601 ctgacattgg cgtggccgag gctgaacggg acgcaggcat ccgggaagct gagtgcaaga 661 aggagatgct ggatgtgaag ttcatggcag acaccaagat tgctgactct aagcgagcct 721 tcgagctgca aaagtcagcc ttcagtgagg aggttaacat caagacagct gaggcccagt 781 tggcctatga gctgcagggg gcccgtgaac agcagaagat ccggcaggaa gagattgaga 841 ttgaggttgt gcagcgcaag aaacagattg ccgtggaggc acaggagatc ctgcgtacgg 901 acaaggagct catcgctaca gtgcgccggc ctgccgaggc cgaggcccac cgcatccagc 961 agattgccga gggtgaaaag gtgaagcagg tcctcttggc acaggcagag gctgagaaga 1021 tccgcaaaat cggggaggcg gaagcgacag tcatcgaggc gatgggcaag gcagaggctg 1081 agcggatgaa gctcaaggca gaagcctacc agaaatacgg gGATGCAGCC AAGATGGCCT 1141 TGGTGCTAGA GGCCCTGCCC CAGATTGCTG CCAAAATCGC TGCCCCACTG CCTGTCCCTT 1201 CTGGTAAAAT AAAGAACTCT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1261 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1321 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTGT AATATTCACA 1381 TTTTTAGTGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt