Construct: ORF TRCN0000468885

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018295.1_s317c1
Derived from:
ccsbBroadEn_10824
DNA Barcode:
GTGTAATATTCACATTTTTAGTGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FLOT2 (2319)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468885
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2319 FLOT2 flotillin 2 NM_004475.3 88.7% 88.1% (many diffs)
2 human 2319 FLOT2 flotillin 2 NM_001330170.2 85.8% 85.8% (many diffs)
3 human 2319 FLOT2 flotillin 2 XM_024450666.1 82.4% 81.8% (many diffs)
4 human 2319 FLOT2 flotillin 2 XM_017024394.1 77.3% 76.7% (many diffs)
5 human 2319 FLOT2 flotillin 2 XM_017024395.1 77.3% 76.7% (many diffs)
6 human 2319 FLOT2 flotillin 2 XM_017024396.1 75% 74.8% (many diffs)
7 human 2319 FLOT2 flotillin 2 XM_005257953.2 70.8% 70.2% (many diffs)
8 human 2319 FLOT2 flotillin 2 XM_024450667.1 70.8% 70.2% (many diffs)
9 mouse 14252 Flot2 flotillin 2 NM_001040403.1 81.7% 87.9% (many diffs)
10 mouse 14252 Flot2 flotillin 2 NM_001284227.1 76.7% 82.3% (many diffs)
11 mouse 14252 Flot2 flotillin 2 NM_001284228.1 71.1% 76.5% (many diffs)
12 mouse 14252 Flot2 flotillin 2 NM_008028.2 71.1% 76.5% (many diffs)
13 mouse 14252 Flot2 flotillin 2 XM_006532192.2 71.1% 76.5% (many diffs)
14 mouse 14252 Flot2 flotillin 2 XM_006532193.3 71.1% 76.5% (many diffs)
15 mouse 14252 Flot2 flotillin 2 XM_006532191.3 68.6% 74.1% (many diffs)
16 mouse 14252 Flot2 flotillin 2 XM_006532194.1 64.6% 70% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1221
ORF length:
1155
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg caattgccac acggtggggc ccaacgaggc gctggtggtt tcagggggct
121gttgtggttc cgactataaa cagtacgtgt ttggcggctg ggcctgggcc tggtggtgta
181tctccgacac tcagaggatt tccctagaga ttatgacgtt gcagccccgc tgcgaggacg
241tagagacggc cgagggggta gctttaactg tgacgggtgt cgcccaggtg aagatcatga
301cggagaagga actcctggcc gtggcttgtg agcagtttct gggtaagaat gtgcaggaca
361tcaaaaacgt cgtcctgcag accctggagg gacatctgcg ctccatcctc gggaccctga
421cagtggagca gatttatcag gaccgggacc agtttgccaa gctggtgcgg gaggtggcag
481cccctgatgt tggccgcatg ggcattgaga tcctcagctt caccatcaag gacgtgtatg
541acaaagtgga ctatctgagc tccctgggca agacgcagac tgccgtggtg cagagagatg
601ctgacattgg cgtggccgag gctgaacggg acgcaggcat ccgggaagct gagtgcaaga
661aggagatgct ggatgtgaag ttcatggcag acaccaagat tgctgactct aagcgagcct
721tcgagctgca aaagtcagcc ttcagtgagg aggttaacat caagacagct gaggcccagt
781tggcctatga gctgcagggg gcccgtgaac agcagaagat ccggcaggaa gagattgaga
841ttgaggttgt gcagcgcaag aaacagattg ccgtggaggc acaggagatc ctgcgtacgg
901acaaggagct catcgctaca gtgcgccggc ctgccgaggc cgaggcccac cgcatccagc
961agattgccga gggtgaaaag gtgaagcagg tcctcttggc acaggcagag gctgagaaga
1021tccgcaaaat cggggaggcg gaagcgacag tcatcgaggc gatgggcaag gcagaggctg
1081agcggatgaa gctcaaggca gaagcctacc agaaatacgg gGATGCAGCC AAGATGGCCT
1141TGGTGCTAGA GGCCCTGCCC CAGATTGCTG CCAAAATCGC TGCCCCACTG CCTGTCCCTT
1201CTGGTAAAAT AAAGAACTCT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1261CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1321AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTGT AATATTCACA
1381TTTTTAGTGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)