Construct: ORF TRCN0000468977
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015983.1_s317c1
- Derived from:
- ccsbBroadEn_02626
- DNA Barcode:
- GTTCGTCTCCGAGGATAGTAATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PKIG (11142)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468977
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11142 | PKIG | cAMP-dependent protein kina... | NM_001281444.1 | 100% | 100% | |
| 2 | human | 11142 | PKIG | cAMP-dependent protein kina... | NM_001281445.2 | 100% | 100% | |
| 3 | human | 11142 | PKIG | cAMP-dependent protein kina... | NM_007066.5 | 100% | 100% | |
| 4 | human | 11142 | PKIG | cAMP-dependent protein kina... | NM_181804.2 | 100% | 100% | |
| 5 | human | 11142 | PKIG | cAMP-dependent protein kina... | NM_181805.2 | 100% | 100% | |
| 6 | human | 11142 | PKIG | cAMP-dependent protein kina... | XM_017027614.1 | 100% | 100% | |
| 7 | human | 11142 | PKIG | cAMP-dependent protein kina... | XM_017027615.1 | 100% | 100% | |
| 8 | human | 11142 | PKIG | cAMP-dependent protein kina... | XM_017027616.1 | 100% | 100% | |
| 9 | mouse | 18769 | Pkig | protein kinase inhibitor, g... | NM_001039390.2 | 88.1% | 90.7% | (many diffs) |
| 10 | mouse | 18769 | Pkig | protein kinase inhibitor, g... | NM_001039391.2 | 88.1% | 90.7% | (many diffs) |
| 11 | mouse | 18769 | Pkig | protein kinase inhibitor, g... | NM_001164053.1 | 88.1% | 90.7% | (many diffs) |
| 12 | mouse | 18769 | Pkig | protein kinase inhibitor, g... | NM_001164055.1 | 88.1% | 90.7% | (many diffs) |
| 13 | mouse | 18769 | Pkig | protein kinase inhibitor, g... | NM_011106.2 | 88.1% | 90.7% | (many diffs) |
| 14 | mouse | 18769 | Pkig | protein kinase inhibitor, g... | XM_006498926.3 | 88.1% | 90.7% | (many diffs) |
| 15 | mouse | 18769 | Pkig | protein kinase inhibitor, g... | XM_006498927.1 | 88.1% | 90.7% | (many diffs) |
| 16 | mouse | 18769 | Pkig | protein kinase inhibitor, g... | NM_001277096.1 | 86.8% | 89.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 220
- ORF end:
- 448
- ORF length:
- 228
- Sequence:
-
1 GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA GACTGAAGTT AGGCCAGCTT 61 GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC 121 TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGCTAGC 181 ATCGATTGAT CAACAAGTTT GTACAAAAAA GTTGGCACCA TGATGGAGGT CGAGTCCTCC 241 TACTCGGACT TCATCTCCTG TGACCGGACA GGCCGTCGGA ATGCGGTCCC TGACATCCAG 301 GGAGACTCAG AGGCTGTGAG CGTGAGGAAG CTGGCTGGAG ACATGGGCGA GCTGGCACTC 361 GAGGGGGCAG AAGGACAGGT GGAGGGAAGC GCCCCAGACA AGGAAGCTGG CAACCAGCCC 421 CAGAGCAGCG ATGGGACCAC CTCGTCTTTG CCAACTTTCT TGTACAAAGT GGTTGATATC 481 GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG 541 TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGTTCGTC 601 TCCGAGGATA GTAATGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg 661 aaagatt