Construct: ORF TRCN0000468987
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017256.1_s317c1
- Derived from:
- ccsbBroadEn_08808
- DNA Barcode:
- TGCGTAGTGGTGGACTTACTCTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL17 (63875)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468987
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 63875 | MRPL17 | mitochondrial ribosomal pro... | NM_022061.4 | 99.8% | 99.4% | 520G>C |
| 2 | mouse | 27397 | Mrpl17 | mitochondrial ribosomal pro... | NM_025301.2 | 84.6% | 84% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 591
- ORF length:
- 525
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gctgtcggtc gctgcagcga tctcccatgg ccgcgtattt cgccgtatgg 121 gcctcggtcc cgagTCCCGC ATCCATCTGT TGCGGAACTT GCTCACAGGG CTGGTGCGGC 181 ACGAACGCAT CGAGGCACCA TGGGCGCGTG TGGACGAAAT GAGGGGCTAC GCGGAGAAGC 241 TCATCGACTA TGGGAAGCTG GGAGACACTA ACGAACGAGC CATGCGCATG GCTGACTTCT 301 GGCTCACAGA GAAGGATTTG ATCCCAAAGC TGTTTCAAGT ACTGGCCCCT CGGTACAAAG 361 ATCAAACTGG GGGCTACACA AGAATGCTGC AGATCCCAAA TCGGAGTTTG GATCGGGCCA 421 AGATGGCAGT GATCGAGTAT AAAGGGAATT GCCTCCCACC CCTGCCTCTG CCTCGCAGAG 481 ACAGCCACCT TACACTCCTA AACCAGCTGC TGCAGGGTTT GCGGCAGGAC CTCAGGCAAA 541 GCCAGGAAGC AAGCAACCAC AGCTCCCACA CAGCTCAAAC ACCACGGATT TACCCAACTT 601 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 661 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 721 CTTGTGGAAA GGACGATGCG TAGTGGTGGA CTTACTCTGC ACGCGTTAAG TCgacaatca 781 acctctggat tacaaaattt gtgaaagatt