Construct: ORF TRCN0000468996

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016932.1_s317c1
Derived from:
ccsbBroadEn_15905
DNA Barcode:
CTAATGCTTATACTGTCAATCCCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KIF16B (55614)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468996
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55614 KIF16B kinesin family member 16B NM_001199865.2 13.6% 13.5% (many diffs)
2 human 55614 KIF16B kinesin family member 16B XM_005260755.3 13.5% 13.3% (many diffs)
3 human 55614 KIF16B kinesin family member 16B XM_005260754.3 13.4% 13.4% 1_3372del
4 human 55614 KIF16B kinesin family member 16B NM_024704.5 13.2% 13.2% 1_3339del;3620_3709del
5 human 55614 KIF16B kinesin family member 16B XM_005260753.3 13.1% 13.1% 1_3372del;3653_3742del
6 human 55614 KIF16B kinesin family member 16B XM_017027926.1 11.7% 10.7% (many diffs)
7 human 55614 KIF16B kinesin family member 16B XM_006723588.3 11.6% 10.6% (many diffs)
8 mouse 16558 Kif16b kinesin family member 16B XM_006498819.3 11.6% 12.1% (many diffs)
9 mouse 16558 Kif16b kinesin family member 16B NM_001081133.2 11.2% 11.6% (many diffs)
10 mouse 16558 Kif16b kinesin family member 16B XM_006498817.3 11.1% 11.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
588
ORF length:
522
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga tgccaggatc aatgcttaca ttgaagaaga agtccaaaga cgccttcagg
121atttgcatcg tgtgattagt gaaggctgca gtacatctgc agacacgatg aaggataatg
181agaaacttca caatggcacc attcaacgta aactaaaata tgagcggatg gtttctcgct
241ctttgggcgc aaatccagat gacctgaagg acccaattaa aattagtatc ccacgctacg
301tcctctgcgg gcaaggaaag gatgcacact tcgagtttga ggtcaagctt gctgcccttg
361aatttcctcc aaagaaacta tttggaaata aggatgaacg tgtgattgct gagagacgaa
421gtcacttaga gaaatacctc agGGACTTTT TCAGCGTGAT GCTCCAGTCC GCAACATCTC
481CCCTCCACAT CAACAAAGTG GGACTGACTC TCTCGAAACA TACCATTTGT GAGTTTTCAC
541CATTCTTCAA GAAAGGAGTC TTTGACTACA GCAGCCACGG GACGGGGTAC CCAACTTTCT
601TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
661CGTAGTAATG AACTAGCCCG TAACTTGAAA GTTTTCGATT TCTTGGCTTT ATATATCTTG
721TGGAAAGGAC GACTAATGCT TATACTGTCA ATCCCCACGC GTTAAGTCga caatcaacct
781ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)