Construct: ORF TRCN0000469027
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013026.1_s317c1
- Derived from:
- ccsbBroadEn_12579
- DNA Barcode:
- CTATCTAAACTCATGATACTCAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SCRN3 (79634)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469027
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79634 | SCRN3 | secernin 3 | NM_024583.5 | 96.7% | 98.1% | (many diffs) |
| 2 | human | 79634 | SCRN3 | secernin 3 | XM_005246854.5 | 96.7% | 98.1% | (many diffs) |
| 3 | human | 79634 | SCRN3 | secernin 3 | XM_005246855.3 | 96.7% | 98.1% | (many diffs) |
| 4 | human | 79634 | SCRN3 | secernin 3 | XM_005246856.3 | 96.7% | 98.1% | (many diffs) |
| 5 | human | 79634 | SCRN3 | secernin 3 | XM_005246857.3 | 96.7% | 98.1% | (many diffs) |
| 6 | human | 79634 | SCRN3 | secernin 3 | XM_017004910.2 | 96.7% | 98.1% | (many diffs) |
| 7 | human | 79634 | SCRN3 | secernin 3 | XM_005246853.3 | 92.4% | 93.7% | (many diffs) |
| 8 | human | 79634 | SCRN3 | secernin 3 | NM_001193528.1 | 92.3% | 93.2% | (many diffs) |
| 9 | human | 79634 | SCRN3 | secernin 3 | XM_011511839.3 | 78.3% | 79.3% | (many diffs) |
| 10 | mouse | 74616 | Scrn3 | secernin 3 | NM_029022.1 | 81.2% | 80% | (many diffs) |
| 11 | mouse | 74616 | Scrn3 | secernin 3 | XM_006500314.2 | 81.2% | 80% | (many diffs) |
| 12 | mouse | 74616 | Scrn3 | secernin 3 | XM_006500313.3 | 77.1% | 75.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1347
- ORF length:
- 1278
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaacctttt tcctgtgaca ctttcgtggc attacctcca gcaacagtcg 121 ataacaggat tatttttgga aaaaattcag atagactcta tgatgaagta caagaggtgg 181 tttattttcc tgctgtagtt catgataacc tgggagaacg tcttaagtgt acatatatag 241 aaattgatca agttcctgaa acatatgctg ttgtcctgag tcgcccagcg tggttgtggg 301 gggcagaaat gggagccaat gagcatggag tttgcattgg gaatgaagct gtatggggaa 361 gagaagaagt ttgtgatgaa gaagcactat taggaatgga ccttgtcaga cttggccttg 421 aaagagctga tacagctgaa aaagccctca atgtcattgt tgacttacta gaaaaatatg 481 gccagggtgg aaattgcaca gagggtagaa tggtatttag ctatcacaac agtttcctga 541 tagctgatag gaatgaagcc tggattctgg agactgcagg gaagtactgg gcagcagaaa 601 aagtacaaga gggagttcgt aatatttcta atcaactttc cataacaacc aagattgccc 661 gggaacaccc agacatgaga aactatgcta agcggaaagg ttggtgggat ggtaaaaagg 721 agtttgattt tgctgcagca tattcctatc ttgacacagc caagatgatg acttcatcag 781 gcagatactg tgagggctac aagcttctaa ataagcacaa aggaaatata acttttgaaa 841 caatgatgga aattcttcga gataaaccaa gtggcattaa tatggaggga gaattcctga 901 ccactgcaag catggtttct attttacctc aagactccag CCTTCCTTGC ATTCACTTCT 961 TTACAGGGAC TCCTGATCCT GAGAGATCTG TTTTTAAGCC TTTCATATTT GTGCCACATA 1021 TTTCACAACT ATTGGATACC AGTTCACCAA CATTTGAACT TGAAGATCTA GTTAAAAAGA 1081 AATCACATTT TAAGCCTGAC AGAAGACACC CACTCTACCA AAAACATCAA CAGGCATTGG 1141 AAGTAGTAAA TAATAATGAG GAAAAAGCCA AAATAATGTT GGACAACATG AGGAAACTGG 1201 AGAAAGAACT ATTCAGAGAG ATGGAATCAA TCCTTCAAAA CAAGCATCTT GATGTGGAGA 1261 AAATTGTTAA TCTCTTTCCT CAGTGTACAA AAGATGAAAT TCAAATTTAT CAGTCAAAGT 1321 TAGTTCTTAG TGATCATATG GTCAGCTAAT ATTAGTTCTT AGTGATCATA TGGTCAGCTT 1381 GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG 1441 TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT 1501 TTATATATCT TGTGGAAAGG ACGACTATCT AAACTCATGA TACTCAATAC GCGTTAAGTC 1561 gacaatcaac ctctggatta caaaatttgt gaaagatt