Construct: ORF TRCN0000469065
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015434.1_s317c1
- Derived from:
- ccsbBroadEn_15146
- DNA Barcode:
- CGCCTTGTCACGCTGGTATCGCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF343 (79175)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469065
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79175 | ZNF343 | zinc finger protein 343 | NM_001282495.1 | 67.2% | 67.1% | 1205T>C;1210_1797del |
| 2 | human | 79175 | ZNF343 | zinc finger protein 343 | NM_001282496.2 | 67.2% | 67.1% | 1205T>C;1210_1797del |
| 3 | human | 79175 | ZNF343 | zinc finger protein 343 | NM_001321800.2 | 67.2% | 67.1% | 1205T>C;1210_1797del |
| 4 | human | 79175 | ZNF343 | zinc finger protein 343 | NM_024325.6 | 67.2% | 67.1% | 1205T>C;1210_1797del |
| 5 | human | 79175 | ZNF343 | zinc finger protein 343 | NM_001321805.2 | 67% | 66.9% | 303_304insGCA;1202T>C;1207_1794del |
| 6 | human | 79175 | ZNF343 | zinc finger protein 343 | NM_001321802.2 | 63% | 62.7% | 305_424del;1325T>C;1330_1917del |
| 7 | human | 79175 | ZNF343 | zinc finger protein 343 | NM_001321803.2 | 63% | 62.7% | 305_424del;1325T>C;1330_1917del |
| 8 | human | 79175 | ZNF343 | zinc finger protein 343 | NM_001282497.2 | 62.9% | 62.8% | 304_426del;1328T>C;1333_1920del |
| 9 | human | 79175 | ZNF343 | zinc finger protein 343 | NM_001321801.2 | 62.9% | 62.8% | 304_426del;1328T>C;1333_1920del |
| 10 | human | 79175 | ZNF343 | zinc finger protein 343 | NM_001282498.1 | 52.1% | 52% | 0_1ins270;935T>C;940_1527del |
| 11 | human | 79175 | ZNF343 | zinc finger protein 343 | NM_001282499.2 | 28.4% | 25% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1275
- ORF length:
- 1209
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gttgccttat ccttcagcac tgggagatca atactgggaa gagattttgc 121 ttccaaagaa tggggaaaat gtagagacta tgaagaaatt gacccaaaat cataaagcga 181 aaggcttgcc ttctaatgat actgactgcc cccagaaaaa ggagggaaag gcccaaatag 241 tggtaccagt tacattcagg gatgtgactg tgatcttcac agaagcagaa tggaagagac 301 tgagtccaga gcagaggaat ctatacaaag aagtgatgct ggagaattac aggaatcttc 361 tctcattggc agaaccaaag ccagaaatct acacttgttc ctcctgcctt ctggccttct 421 cctgtcagca gttcctcagt caacatgtac ttcagatctt cctgggctta tgtgcagaaa 481 atcacttcca tccagggaat tctagcccag ggcattggaa acagcagggg cagcagtatt 541 cccatgtaag ctgttggttt gaaaatgcag aaggtcagga gagaggaggt ggctccaaac 601 cctggtctgc aaggacagag gagagagaaa cctcaagggc attccccagc ccactccaaa 661 gacagtcagc aagtcctaga aaaggcaaca tggtggtaga aacagagccc agctcagccc 721 aaagaccaaa ccctgtgcag ctagacaaag gcttgaagga attagaaacc ttgagatttg 781 gagcaatcaa ctgtagagag tatgaaccgg accataacct ggaatcaaac tttattacaa 841 acccgaggac cctcttaggg aagaagccct acatttgcag tgattgtggg cgaagcttta 901 aagatagatc aaccctcatc agacaccatc gtatacactc gatggagaag cctTATGTGT 961 GCAGTGAGTG CGGGCGAGGT TTTAGCCAGA AGTCCAACCT CAGCAGACAC CAGAGAACAC 1021 ATTCAGAAGA GAAGCCTTAT TTGTGCAGGG AGTGTGGGCA AAGCTTTAGA AGTAAGTCCA 1081 TCCTCAATAG ACATCAGTGG ACTCACTCAG AGGAGAAGCC CTATGTTTGC AGCGAGTGTG 1141 GGCGAGGCTT TAGCGAGAAG TCATCCTTCA TCAGACACCA GAGGACACAC TCCGGTGAGA 1201 AACCCTATGT GTGCCTGGAG TGTGGACGAA GCTTTTGTGA TAAGTCAACC CTCAGAAAAC 1261 ACCAGAGGAC ACACTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1321 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1381 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGCCTTGTCA CGCTGGTATC 1441 GCTAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt