Construct: ORF TRCN0000469125
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016360.1_s317c1
- Derived from:
- ccsbBroadEn_03551
- DNA Barcode:
- GCATAAAGATCGCATCCGCGAGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMLHE (55217)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469125
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55217 | TMLHE | trimethyllysine hydroxylase... | NM_001184797.2 | 100% | 100% | |
| 2 | human | 55217 | TMLHE | trimethyllysine hydroxylase... | NM_018196.4 | 84.2% | 77.9% | (many diffs) |
| 3 | human | 55217 | TMLHE | trimethyllysine hydroxylase... | XM_011531182.3 | 71.2% | 64.6% | (many diffs) |
| 4 | human | 55217 | TMLHE | trimethyllysine hydroxylase... | XM_017029620.2 | 68.2% | 67.5% | (many diffs) |
| 5 | human | 55217 | TMLHE | trimethyllysine hydroxylase... | XR_247318.3 | 37.8% | (many diffs) | |
| 6 | mouse | 192289 | Tmlhe | trimethyllysine hydroxylase... | NM_138758.1 | 75.2% | 68.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1194
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gtaccacaga ttgtcccacc tacacagcag gcttcaggac ttgctgaagg 121 gaggagtcat atatccggcc cttccacagc ccaacttcaa aagcttactt cctttagctg 181 tccattggca ccatacagcc tccaagtctc tgacttgtgc ttggcagcaa catgaagatc 241 attttgagct gaaatatgct aataccgtga tgcgctttga ttacgtctgg cttcgagacc 301 actgccgctc agcatcgtgc tacaactcta agactcacca gcgcagcctg gatactgcca 361 gtgtggattt atgtatcaag ccaaagacca ttcgtctgga tgagaccaca ctctttttca 421 cttggccaga tggtcatgtg actaaatatg atttgaattg gctggtgaaa aacagctatg 481 aagggcagaa acaaaaagtc atccagccta gaatactatg gaatgctgaa atctaccagc 541 aagcccaagt tccatcggta gattgccaga gcttcttaga aaccaacgag ggactgaaga 601 agtttctgca aaactttctg ctctatggaa ttgcattcgt agaaaatgtc cctcccactc 661 aagagcacac agagaagttg gcagaaagga tcagcttaat cagagaaacc atttatggga 721 ggatgtggta tttcacttca gacttctcca gaggtgacac tgcgtacacc aagctagctc 781 tggatcggca cactgacact acctattttc aagagcccTG TGGCATTCAA GTGTTTCATT 841 GTCTTAAACA TGAAGGAACT GGTGGCAGGA CACTGCTAGT AGATGGATTC TATGCAGCAG 901 AACAGGTACT TCAAAAGGCA CCTGAGGAAT TTGAACTCCT CAGTAAAGTG CCATTGAAGC 961 ATGAATATAT TGAAGATGTT GGAGAATGTC ACAACCACAT GATTGGGATT GGGCCAGTCT 1021 TAAATATCTA CCCATGGAAT AAAGAGCTGT ATTTGATCAG ATTATTCAAA GAAAAACAAA 1081 ACACGGTCAA CAGGCAGTGG AACTCCTCAC TCCAATGTGA TATTCCTGAG AGAATATTGA 1141 CTTATCGTCA CTTCGTCTCT GGGACAAGTA TTGAACATAG GGGAAGCCTT ATATACCCAA 1201 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1261 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1321 TATCTTGTGG AAAGGACGAG CATAAAGATC GCATCCGCGA GCTACGCGTT AAGTCgacaa 1381 tcaacctctg gattacaaaa tttgtgaaag att