Construct: ORF TRCN0000469126
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005801.1_s317c1
- Derived from:
- ccsbBroadEn_12576
- DNA Barcode:
- AAAAAACACTCAGTGTGAAACCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDNF (79625)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469126
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79625 | NDNF | neuron derived neurotrophic... | NM_024574.4 | 58% | 58.2% | (many diffs) |
| 2 | human | 79625 | NDNF | neuron derived neurotrophic... | XM_024454212.1 | 58% | 58.2% | (many diffs) |
| 3 | human | 79625 | NDNF | neuron derived neurotrophic... | XM_024454213.1 | 58% | 58.2% | (many diffs) |
| 4 | mouse | 68169 | Ndnf | neuron-derived neurotrophic... | NM_172399.3 | 49.8% | 51.2% | (many diffs) |
| 5 | mouse | 68169 | Ndnf | neuron-derived neurotrophic... | XM_006506528.3 | 49.8% | 51.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1059
- ORF length:
- 993
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ggcaccgaaa cctggtctgg acttcagccc ctttgacttt gcccactttg 121 gatttccttc tgataattca ggtaaagaac gcagtttcca ggcaaagcct tctccaaaac 181 tggggcgtca tgtctactcc aggcccaagg ttgatattca gaaaatctgc ataggaaaca 241 agaacatctt caccgtctct gatctgaaac ccgacacgca gtactacttt gacgtatttg 301 tggtcaacat caacagcaac atgagcaccg cttatgtagg tacctttgcc aggaccaagg 361 aagaagccaa acagaagaca gtcgagctca aagatgggaa gataacagat gtatttgtta 421 aaaggaaggg agcaaagttt ctacggtttg ctccagtctc ttctcaccaa aaagtcacct 481 tctttattca ctcttgtctg gatgctgtcc aaatccaagt gagaagagat gggaaacttc 541 ttctgtctca gaatgtggaa ggcattcagc agtttcagct tagaggaaaa cctaaagcta 601 aatacctcgt tcgactgaaa ggaaacaaga aaggagcatc tatgttgaaa aTTCTAGCTA 661 CCACAAGGCC TACTAAGCAG TCATTTCCCT CTCTTCCTGA AGACACAAGA ATCAAAGCCT 721 TTGACAAGCT CCGTACCTGT TCCTCGGCCA CCGTGGCTTG GCTAGGCACT CAGGAAAGGA 781 ACAAGTTTTG CATCTACAAA AAAGAAGTGG ATGATAACTA CAATGAAGAC CAGAAGAAAA 841 GAGAGCAAAA CCAATGTCTA GGACCAGATA TAAGGAAGAA GTCAGAAAAG GTCCTCTGTA 901 AATATTTCCA CAGTCAAAAC CTGCAGAAAG CAGTGACCAC AGAAACAATT AAAGGTCTTC 961 AGCCTGGCAA ATCTTACCTG CTGGATGTTT ATGTCATAGG ACATGGGGGG CACTCTGTAA 1021 AGTATCAGAG TAAGGTTGTG AAAACTAGAA AGTTCTGTTA CCCAACTTTC TTGTACAAAG 1081 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1141 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1201 ACGAAAAAAA CACTCAGTGT GAAACCGCAC GCGTTAAGTC gacaatcaac ctctggatta 1261 caaaatttgt gaaagatt