Transcript: Human NM_024574.4

Homo sapiens neuron derived neurotrophic factor (NDNF), mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
NDNF (79625)
Length:
2902
CDS:
545..2251

Additional Resources:

NCBI RefSeq record:
NM_024574.4
NBCI Gene record:
NDNF (79625)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_024574.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000152481 CGTTCGACTGAAAGGAAACAA pLKO.1 1798 CDS 100% 5.625 7.875 N NDNF n/a
2 TRCN0000150752 GTATCAGAGTAAGGTTGTGAA pLKO.1 2212 CDS 100% 4.950 6.930 N NDNF n/a
3 TRCN0000150994 CCCAAGGTTGATATTCAGAAA pLKO.1 1394 CDS 100% 4.950 3.960 N NDNF n/a
4 TRCN0000151353 GCATAGGAAACAAGAACATCT pLKO.1 1419 CDS 100% 4.950 3.960 N NDNF n/a
5 TRCN0000151107 GTACTATGGAAGGAAGGATAT pLKO.1 2359 3UTR 100% 10.800 7.560 N NDNF n/a
6 TRCN0000151920 CAAATCCAAGTGAGAAGAGAT pLKO.1 1700 CDS 100% 4.950 3.465 N NDNF n/a
7 TRCN0000158163 CCCTTTGACTTTGCCCACTTT pLKO.1 1289 CDS 100% 4.950 3.465 N NDNF n/a
8 TRCN0000153470 CTCTTCCTGAAGACACAAGAA pLKO.1 1881 CDS 100% 4.950 3.465 N NDNF n/a
9 TRCN0000151108 GAAGGATATCAACGTGTGTAT pLKO.1 2371 3UTR 100% 4.950 3.465 N NDNF n/a
10 TRCN0000152117 CCTAAAGCTAAATACCTCGTT pLKO.1 1781 CDS 100% 2.640 1.848 N NDNF n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_024574.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12576 pDONR223 100% 58% 58.2% None (many diffs) n/a
2 ccsbBroad304_12576 pLX_304 0% 58% 58.2% V5 (many diffs) n/a
3 TRCN0000469126 AAAAAACACTCAGTGTGAAACCGC pLX_317 41.2% 58% 58.2% V5 (many diffs) n/a
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