Construct: ORF TRCN0000469133
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014384.1_s317c1
- Derived from:
- ccsbBroadEn_06893
- DNA Barcode:
- CCCGAAGAAAGTCTACAGATGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS4Y1 (6192)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469133
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6192 | RPS4Y1 | ribosomal protein S4 Y-link... | NM_001008.4 | 99.7% | 99.6% | 771_772delCAinsTG |
| 2 | human | 140032 | RPS4Y2 | ribosomal protein S4 Y-link... | NM_001039567.3 | 93.7% | 94.2% | (many diffs) |
| 3 | human | 6191 | RPS4X | ribosomal protein S4 X-linked | NM_001007.5 | 82.2% | 93.1% | (many diffs) |
| 4 | mouse | 20102 | Rps4x | ribosomal protein S4, X-linked | NM_009094.2 | 81.1% | 93.1% | (many diffs) |
| 5 | mouse | 20102 | Rps4x | ribosomal protein S4, X-linked | XM_011247553.2 | 75.6% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 858
- ORF length:
- 789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcccggggc cccaagaagc acttaaagcg tgttgcagcg ccgaagcatt 121 ggatgcttga caaactaacg ggtgtatttg cacctcgtcc atcgacaggt ccccacaagc 181 tgagggaatg tcttcctctg atcgtcttcc tcaggaatag actcaagtat gcgttgactg 241 gagatgaggt aaagaagata tgtatgcaac gtttcatcaa aattgatggc aaggttcgag 301 tggatgtcac ataccctgct ggattcatgg atgtcatcag catcgagaag acaggtgaac 361 atttccgcct ggtctatgac accaagggcc gttttgctgt tcaccgcatc acagtggaag 421 aggcaaagta caagttgtgc aaagtgagga agattactgt gggagtgaag ggaaTCCCTC 481 ACCTGGTGAC TCATGATGCT CGAACCATCC GCTACCCAGA TCCTGTCATC AAGGTGAACG 541 ATACTGTGCA GATTGATTTA GGGACTGGCA AGATAATCAA CTTTATCAAA TTTGATACAG 601 GCAATTTGTG TATGGTGATT GGTGGAGCCA ACCTCGGTCG TGTTGGTGTG ATCACCAACA 661 GGGAAAGACA TCCTGGTTCT TTTGATGTGG TGCATGTGAA GGATGCCAAT GGCAACAGCT 721 TTGCCACGAG GCTTTCCAAC ATTTTTGTCA TTGGCAATGG CAATAAACCT TGGATTTCCC 781 TGCCCAGGGG AAAGGGCATT CGACTTACTG TTGCTGAAGA GAGAGATAAG AGGCTGGCTG 841 CCAAACAGAG CAGTGGCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCCGAAG AAAGTCTACA 1021 GATGCCTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt