Construct: ORF TRCN0000469135
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000646.1_s317c1
- Derived from:
- ccsbBroadEn_12031
- DNA Barcode:
- AACATACCTTGTTGTCATTTATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2BS1 (54145)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469135
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54145 | H2BS1 | H2B.S histone 1 | NM_017445.3 | 100% | 100% | |
| 2 | human | 102724334 | LOC102724334 | histone H2B type F-S-like | XM_006723918.4 | 100% | 100% | |
| 3 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_001312653.1 | 98.9% | 98.4% | (many diffs) |
| 4 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_080593.2 | 98.9% | 98.4% | (many diffs) |
| 5 | human | 8344 | H2BC6 | H2B clustered histone 6 | NM_003523.2 | 93.6% | 97.6% | (many diffs) |
| 6 | human | 8340 | H2BC13 | H2B clustered histone 13 | NM_003519.3 | 92.3% | 96% | (many diffs) |
| 7 | human | 8970 | H2BC11 | H2B clustered histone 11 | NM_021058.3 | 92% | 97.6% | (many diffs) |
| 8 | human | 8341 | H2BC15 | H2B clustered histone 15 | NM_003520.3 | 91.7% | 96.8% | (many diffs) |
| 9 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001024599.4 | 91.5% | 96% | (many diffs) |
| 10 | human | 8343 | H2BC7 | H2B clustered histone 7 | NM_003522.3 | 91% | 97.6% | (many diffs) |
| 11 | human | 8348 | H2BC17 | H2B clustered histone 17 | NM_003527.4 | 91% | 96% | (many diffs) |
| 12 | human | 8345 | H2BC9 | H2B clustered histone 9 | NM_003524.2 | 90.7% | 96.8% | (many diffs) |
| 13 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_021063.3 | 90.7% | 96.8% | (many diffs) |
| 14 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_138720.2 | 90.7% | 96.8% | (many diffs) |
| 15 | human | 8349 | H2BC21 | H2B clustered histone 21 | NM_003528.2 | 90.4% | 96.8% | (many diffs) |
| 16 | human | 8346 | H2BC10 | H2B clustered histone 10 | NM_003525.2 | 90.2% | 97.6% | (many diffs) |
| 17 | human | 8347 | H2BC4 | H2B clustered histone 4 | NM_003526.2 | 90.2% | 97.6% | (many diffs) |
| 18 | human | 8342 | H2BC14 | H2B clustered histone 14 | NM_003521.2 | 87.8% | 94.4% | (many diffs) |
| 19 | human | 8339 | H2BC8 | H2B clustered histone 8 | NM_003518.3 | 87.5% | 97.6% | (many diffs) |
| 20 | human | 128312 | H2BU1 | H2B.U histone 1 | NM_175055.2 | 87.3% | 92.8% | (many diffs) |
| 21 | human | 3018 | H2BC3 | H2B clustered histone 3 | NM_021062.2 | 86.5% | 95.2% | (many diffs) |
| 22 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001161334.1 | 86% | 90.2% | (many diffs) |
| 23 | human | 3017 | H2BC5 | H2B clustered histone 5 | XM_005249039.4 | 76.2% | 81.3% | (many diffs) |
| 24 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160944.2 | 68.9% | (many diffs) | |
| 25 | human | 337873 | H2BC20P | H2B clustered histone 20, p... | NR_036461.1 | 58.1% | (many diffs) | |
| 26 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160915.1 | 21.4% | (many diffs) | |
| 27 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_027337.2 | 18.8% | (many diffs) | |
| 28 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160941.1 | 17.4% | (many diffs) | |
| 29 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160942.1 | 16.1% | (many diffs) | |
| 30 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160940.1 | 15.7% | (many diffs) | |
| 31 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160913.1 | 14% | (many diffs) | |
| 32 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160914.1 | 11.5% | (many diffs) | |
| 33 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_001290380.1 | 92.3% | 97.6% | (many diffs) |
| 34 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_023422.3 | 92.3% | 97.6% | (many diffs) |
| 35 | mouse | 319181 | Hist1h2bg | histone cluster 1, H2bg | NM_178196.4 | 91.7% | 97.6% | (many diffs) |
| 36 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001313878.1 | 91.7% | 96.8% | (many diffs) |
| 37 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001313880.1 | 91.7% | 96.8% | (many diffs) |
| 38 | mouse | 319180 | Hist1h2bf | histone cluster 1, H2bf | NM_178195.2 | 91.7% | 96.8% | (many diffs) |
| 39 | mouse | 319186 | Hist1h2bm | histone cluster 1, H2bm | NM_178200.2 | 91.7% | 96.8% | (many diffs) |
| 40 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001177653.1 | 91.5% | 97.6% | (many diffs) |
| 41 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001290530.1 | 91.5% | 97.6% | (many diffs) |
| 42 | mouse | 319184 | Hist1h2bk | histone cluster 1, H2bk | NM_175665.2 | 91.5% | 97.6% | (many diffs) |
| 43 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_178194.4 | 91.5% | 97.6% | (many diffs) |
| 44 | mouse | 319185 | Hist1h2bl | histone cluster 1, H2bl | NM_178199.2 | 91.5% | 96.8% | (many diffs) |
| 45 | mouse | 319182 | Hist1h2bh | histone cluster 1, H2bh | NM_178197.2 | 91.2% | 96.8% | (many diffs) |
| 46 | mouse | 319183 | Hist1h2bj | histone cluster 1, H2bj | NM_178198.2 | 91.2% | 96.8% | (many diffs) |
| 47 | mouse | 319187 | Hist1h2bn | histone cluster 1, H2bn | NM_178201.2 | 91% | 96.8% | (many diffs) |
| 48 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_001290466.1 | 90.2% | 95.2% | (many diffs) |
| 49 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | XM_006516690.2 | 90.2% | 95.2% | (many diffs) |
| 50 | mouse | 319178 | Hist1h2bb | histone cluster 1, H2bb | NM_175664.3 | 89.9% | 94.4% | (many diffs) |
| 51 | mouse | 319189 | Hist2h2bb | histone cluster 2, H2bb | NM_175666.2 | 88.6% | 96% | (many diffs) |
| 52 | mouse | 78303 | Hist3h2ba | histone cluster 3, H2ba | NM_030082.4 | 86.3% | 91.2% | (many diffs) |
| 53 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001097979.2 | 86% | 90.2% | (many diffs) |
| 54 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001110555.1 | 86% | 90.2% | (many diffs) |
| 55 | mouse | 319190 | Hist2h2be | histone cluster 2, H2be | NM_178214.4 | 85.4% | 95.2% | (many diffs) |
| 56 | mouse | 319177 | Hist1h2ba | histone cluster 1, H2ba | NM_175663.2 | 84.5% | 86.6% | (many diffs) |
| 57 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_178202.2 | 82.1% | 86.9% | (many diffs) |
| 58 | mouse | 382522 | Hist3h2bb-ps | histone cluster 3, H2bb, ps... | NR_134309.1 | 70.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 471
- ORF length:
- 378
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGCCGGA GCCAGCGAAG TCCGCTCCCG 121 CGCCCAAGAA GGGCTCGAAG AAAGCCGTGA CTAAGGCGCA GAAGAAGGAC GGCAGGAAGC 181 GCAAGCGCAG CCGCAAGGAG AGCTACTCCG TATACGTGTA CAAGGTGCTG AAGCAGGTCC 241 ACCCCGACAC CGGCATCTCC TCTAAGGCCA TGGGAATCAT GAACTCCTTC GTCAACGACA 301 TCTTCGAACG CATCGCAGGT GAGGCTTCCC GCCTGCCGCA TTACAACAAG CGCTCGACCA 361 TCACCTCCAG GGAGATCCAG ACGGCCGTGC GCCTGCTGCT GCCCGGGGAG TTGGCCAAGC 421 ACGCCGTGTC CGAGGGCACC AAGGCCGTCA CCAAGTACAC CAGCGCTAAG TTGCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 601 CTTGTGGAAA GGACGAAACA TACCTTGTTG TCATTTATTA ACGCGTTAAG TCgacaatca 661 acctctggat tacaaaattt gtgaaagatt