Construct: ORF TRCN0000469145
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007354.1_s317c1
- Derived from:
- ccsbBroadEn_08408
- DNA Barcode:
- GTTTAAAAACTTCCCGTATGGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM44 (54765)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469145
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54765 | TRIM44 | tripartite motif containing 44 | NM_017583.6 | 99.9% | 100% | 879T>G |
| 2 | human | 54765 | TRIM44 | tripartite motif containing 44 | XM_006718254.1 | 76.7% | 76.7% | 747_748ins240 |
| 3 | mouse | 80985 | Trim44 | tripartite motif-containing 44 | NM_020267.2 | 88.1% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1098
- ORF length:
- 1032
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctctggagtg ggcgcggcct tcgaggaact gcctcacgac ggcacgtgtg 121 acgagtgcga gcccgacgag gctccggggg ccgaggaagt gtgccgagaa tgcggcttct 181 gctactgccg ccgccatgcc gaggcgcaca ggcagaagtt cctcagtcac catctggccg 241 aatacgtcca cggctcccag gcctggaccc cgccagctga cggagagggg gcggggaagg 301 aagaagcgga ggtcaaggtg gagcaggaga gggagataga aagcgaggca ggggaagaga 361 gtgagtcgga ggaagagagc gagtcagagg aagagagcga gacagaggaa gagagtgagg 421 atgagagcga tgaggagagt gaagaagaca gcgaggaaga aatggaggat gagcaagaaa 481 gcgaggccga agaagacaac caagaagaag gggaatccga ggcggaggga gaaactgagg 541 cagaaagtga atttgaccca gaaatagaaa tggaagcaga gagagtggcc aagaggaagt 601 gtccggacca tgggcttgat ttgagtacct attgccagga agataggcag ctcatctgtg 661 tcctgtgtcc agtcattggg gctcaccagg gccaccaact ctccacccta gacgaagcct 721 ttgaAGAATT AAGAAGCAAA GACTCAGGTG GACTGAAGGC CGCTATGATC GAATTGGTGG 781 AAAGGTTGAA GTTCAAGAGC TCAGACCCTA AAGTAACTCG GGACCAAATG AAGATGTTTA 841 TACAGCAGGA ATTTAAGAAA GTTCAGAAAG TGATTGCTGA TGAGGAGCAG AAGGCCCTTC 901 ATCTAGTGGA CATCCAAGAG GCAATGGCCA CAGCTCATGT GACGGAGATA CTGGCAGACA 961 TCCAATCCCA CATGGATAGG TTGATGACTC AGATGGCCCA AGCCAAGGAA CAACTTGATA 1021 CCTCTAATGA ATCAGCTGAG CCAAAGGCAG AGGGCGATGA GGAAGGACCC AGTGGTGCCA 1081 GTGAAGAAGA GGACACATGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1141 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1201 GTATTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTTTAAAA ACTTCCCGTA 1261 TGGTTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt