Construct: ORF TRCN0000469165
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008887.1_s317c1
- Derived from:
- ccsbBroadEn_08026
- DNA Barcode:
- CTCTACGTTAAGCCTTTGGCGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR10H2 (26538)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469165
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26538 | OR10H2 | olfactory receptor family 1... | NM_013939.2 | 99.7% | 100% | 114G>A;321C>T |
| 2 | human | 284433 | OR10H5 | olfactory receptor family 1... | NM_001004466.1 | 90.9% | 87.3% | (many diffs) |
| 3 | human | 26539 | OR10H1 | olfactory receptor family 1... | NM_013940.3 | 88.5% | 86.7% | (many diffs) |
| 4 | human | 26539 | OR10H1 | olfactory receptor family 1... | XM_011527911.1 | 88.5% | 86.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1014
- ORF length:
- 945
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctggggcta aaccacacct ccatgtctga attcatcctc gtcggcttct 121 ctgccttccc ccacctccaa ctgatgctct tcctgctgtt cctgctgatg tacctgttca 181 cactgctggg caacctgctc atcatggcca ccgtctggag cgagcgcagc ctccacacgc 241 ccatgtacct cttcctgtgc gtcctctcag tctccgagat cctctacacc gtggccatca 301 tcccgcgcat gctggccgac ctgctgtcca cccagcgctc catcgccttc ctggcctgtg 361 ccagtcagat gttcttctcc ttcagctttg gcttcaccca ctccttcctg ctcaccgtca 421 tgggctacga ccgctacgtg gccatctgcc accccctgcg ctacaacgtg ctcatgagcc 481 cacggggctg cgcctgcctg gtgggctgct cctgggctgg tggctcggtc atggggatgg 541 tggtgacctc ggccattttc caactgactt tctgtggatc ccatgagatc cagcattttt 601 tatgtcatgt gccacctctg ttgaagttgg cctgtggaaa taatgtacca gctgtggccc 661 tgggcgtggg cttggtatgt atcatggcac tgctgggctg ttttctccTC ATCCTCCTCT 721 CCTATGCCTT CATCGTGGCC GACATCTTGA AGATCCCTTC TGCTGAAGGT CGGAACAAGG 781 CCTTCTCCAC CTGTGCCTCT CACCTTATTG TGGTCATTGT GCACTATGGC TTTGCCTCTG 841 TCATCTACCT CAAGCCCAAA GGTCCCCACT CTCAGGAGGG TGACACCCTG ATGGCCACCA 901 CCTACGCAGT CCTCACGCCC TTCCTCAGCC CCATCATCTT CAGCCTCAGG AACAAAGAAC 961 TGAAGGTTGC CATGAAGAGG ACCTTCCTCA GCACACTCTA TTCCTCAGGC ACCTTGCCAA 1021 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1081 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1141 TATCTTGTGG AAAGGACGAC TCTACGTTAA GCCTTTGGCG TCTACGCGTT AAGTCgacaa 1201 tcaacctctg gattacaaaa tttgtgaaag att