Construct: ORF TRCN0000469244
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003927.1_s317c1
- Derived from:
- ccsbBroadEn_15839
- DNA Barcode:
- GTTCACACACCAAGACTTGCGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANAPC5 (51433)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469244
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51433 | ANAPC5 | anaphase promoting complex ... | NM_016237.5 | 99.9% | 100% | 1525C>T |
| 2 | human | 51433 | ANAPC5 | anaphase promoting complex ... | NM_001330489.2 | 98.2% | 98.2% | 1121_1122ins39;1486C>T |
| 3 | human | 51433 | ANAPC5 | anaphase promoting complex ... | NM_001137559.1 | 85.1% | 85.1% | 0_1ins297;824_825ins39;1189C>T |
| 4 | human | 51433 | ANAPC5 | anaphase promoting complex ... | XM_017019423.2 | 47.2% | 47.2% | 0_1ins1194;331C>T |
| 5 | human | 51433 | ANAPC5 | anaphase promoting complex ... | XM_017019424.2 | 47.2% | 47.2% | 0_1ins1194;331C>T |
| 6 | mouse | 59008 | Anapc5 | anaphase-promoting complex ... | NM_021505.3 | 88.3% | 92.8% | (many diffs) |
| 7 | mouse | 59008 | Anapc5 | anaphase-promoting complex ... | NM_001289518.1 | 87.3% | 91.7% | (many diffs) |
| 8 | mouse | 59008 | Anapc5 | anaphase-promoting complex ... | NM_001289520.1 | 87.2% | 91.7% | (many diffs) |
| 9 | mouse | 59008 | Anapc5 | anaphase-promoting complex ... | NM_001042491.2 | 86.5% | 91.1% | (many diffs) |
| 10 | mouse | 59008 | Anapc5 | anaphase-promoting complex ... | NM_001289517.1 | 85.6% | 90% | (many diffs) |
| 11 | mouse | 59008 | Anapc5 | anaphase-promoting complex ... | XM_017321048.1 | 80.8% | 84.2% | (many diffs) |
| 12 | mouse | 59008 | Anapc5 | anaphase-promoting complex ... | NM_001289519.1 | 78.9% | 71.5% | (many diffs) |
| 13 | mouse | 59008 | Anapc5 | anaphase-promoting complex ... | XR_387575.1 | 75.7% | (many diffs) | |
| 14 | mouse | 59008 | Anapc5 | anaphase-promoting complex ... | XM_006530429.3 | 74.7% | 64.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2331
- ORF length:
- 2265
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagcgtccac gagagcctct acttcaatcc catgatgacc aatggggttg 121 tgcacgccaa tgtgttcggc atcaaggact gggtgacgcc gtacaagatc gcggtgctgg 181 tgctgctgaa cgagatgagc cgcacaggcg agggcgccgt cagcctcatg gagcggcgga 241 ggctcaacca gctgctcctg cccctgctgc agggcccaga tattacactg tcaaaacttt 301 acaagttaat tgaagagtct tgtccacagc tggcaaattc agtgcagatc agaatcaaac 361 tgatggctga aggcgagttg aaggatatgg aacagttttt tgatgacctt tcagattctt 421 tctctggaac tgaaccagag gttcacaaaa caagtgtagt aggtttgttt ctgcgtcaca 481 tgatcttggc ctacagtaag ctttctttca gccaagtgtt taaactgtac actgcccttc 541 agcagtactt ccagaatggt gagaaaaaga cagtggagga tgctgatatg gaactgacca 601 gtagagatga gggtgaaaga aaaatggaaa aagaagaact tgatgtatct gtaagagaag 661 aggaggtatc ttgcagtggg cctctgtccc aaaaacaagc agaatttttt ctttctcaac 721 aggcttcttt gctaaagaat gatgagacta aggccctcac tccagcttcc ttgcagaagg 781 aattaaacaa tttgttgaaa tttaatcctg attttgctga agcgcattat ctcagctact 841 taaacaacct ccgtgtccaa gatgttttca gttcaacaca cagtctcctc cattattttg 901 atcgtctgat tcttaccgga gccgaaagca aaagtaatgg ggaagagggc tatggccgga 961 gcttgagata cgccgctctg aatcttgccg ccctgcactg ccgcttcggt cactatcaac 1021 aggcagagct cgccctgcag gaggcaatta ggattgccca ggagtccaac gatcacgtgt 1081 gtctccagca ctgtttgagc tggctttatg tgctggggca gaagagatcc gatagctatg 1141 ttctgctgga gcattctgtg aagaaggcag tacattttgg gttaccgtac ctcgcctccc 1201 tgggaataca gtcccttgtt caacagagag cttttgctgg gaagacggca aacaagctga 1261 tggatgccct aaaggactcc gacctcctgc actggaaaca cagcctgtca gagctcatcg 1321 atatcagcat cgcacagaaa acggccatct ggaggctgta tggccgcagc accatggcac 1381 tgcaacaggc ccagatgttg ctgagcatga acagcctgga ggcggtgaat gcgggcgtgc 1441 agcagaacaa cacagagtcc tttgctgtcg cactctgcca cctcgcagag ctacacgcgg 1501 agcagggctg ttttgctgca gcttctgaag tgttaaagca cttgaaggaa cgatttccgc 1561 ctaatagtca gcacgcccag ttatggatgt tatgtgatca aaaaatacag tttgacagag 1621 caatgaatga tggcaaatat catttggctg attcacttgt tacaggaatc acagctctca 1681 atagcataga gggtgtttat aggaaagcgg ttgtattaca agctcagaac caaatgtcag 1741 aggcacataa gcttttacaa aaattgttgg ttcattgtca gaaactgaag aacacagaaa 1801 tggtgatcag tgtcctactg tccgtggcag agctgtactg gcgatcttcc tcccctacca 1861 tcgcgctgcc catgctcctg caggctctgg ccctctccaa ggagtaccgg ttacagtact 1921 tggccTCTGA AACAGTGCTG AACTTGGCTT TTGCGCAGCT CATTCTTGGA ATCCCAGAAC 1981 AGGCCTTAAG TCTTCTCCAC ATGGCCATCG AGCCCATCTT GGCTGACGGG GCTATCCTGG 2041 ACAAAGGTCG TGCCATGTTC TTAGTGGCCA AGTGCCAGGT GGCTTCAGCA GCTTCCTACG 2101 ATCAGCCGAA GAAAGCAGAA GCTCTGGAGG CTGCCATCGA GAACCTCAAT GAAGCCAAGA 2161 ACTATTTTGC AAAGGTTGAC TGCAAAGAGC GCATCAGGGA CGTCGTTTAC TTCCAGGCCA 2221 GACTCTACCA TACCCTGGGG AAGACCCAGG AGAGGAACCG GTGTGCGATG CTCTTCCGGC 2281 AGCTGCATCA GGAGCTGCCC TCTCATGGGG TACCCTTGAT AAACCATCTC TACCCAACTT 2341 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2401 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2461 CTTGTGGAAA GGACGAGTTC ACACACCAAG ACTTGCGCAA ACGCGTTAAG TCgacaatca 2521 acctctggat tacaaaattt gtgaaagatt