Transcript: Mouse XM_006530429.3

PREDICTED: Mus musculus anaphase-promoting complex subunit 5 (Anapc5), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Anapc5 (59008)
Length:
2376
CDS:
188..2056

Additional Resources:

NCBI RefSeq record:
XM_006530429.3
NBCI Gene record:
Anapc5 (59008)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006530429.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000087995 GCACTGTTTGAGCTGGCTTTA pLKO.1 1165 CDS 100% 10.800 15.120 N Gm10482 n/a
2 TRCN0000088405 GCCGCTTTGTTGAAGAATGAT pLKO.1 800 CDS 100% 5.625 7.875 N Anapc5 n/a
3 TRCN0000325253 GCCGCTTTGTTGAAGAATGAT pLKO_005 800 CDS 100% 5.625 7.875 N Anapc5 n/a
4 TRCN0000088407 CTGAAGGATATGGAACAATTT pLKO.1 464 CDS 100% 13.200 9.240 N Anapc5 n/a
5 TRCN0000325252 CTGAAGGATATGGAACAATTT pLKO_005 464 CDS 100% 13.200 9.240 N Anapc5 n/a
6 TRCN0000088406 GCTCATTACCTCAGCTACTTA pLKO.1 899 CDS 100% 5.625 3.938 N Anapc5 n/a
7 TRCN0000325255 GCTCATTACCTCAGCTACTTA pLKO_005 899 CDS 100% 5.625 3.938 N Anapc5 n/a
8 TRCN0000087932 CAGAACAATACTGAGTCCTTT pLKO.1 1520 CDS 100% 4.950 3.465 N Gm5288 n/a
9 TRCN0000088404 GCCCAGATATTACACTGTCAA pLKO.1 360 CDS 100% 4.950 3.465 N Anapc5 n/a
10 TRCN0000325254 GCCCAGATATTACACTGTCAA pLKO_005 360 CDS 100% 4.950 3.465 N Anapc5 n/a
11 TRCN0000088403 GCCAAGAACTACTTTGCACAA pLKO.1 2109 3UTR 100% 4.050 2.835 N Anapc5 n/a
12 TRCN0000325251 GCCAAGAACTACTTTGCACAA pLKO_005 2109 3UTR 100% 4.050 2.835 N Anapc5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006530429.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15839 pDONR223 0% 74.7% 64.1% None (many diffs) n/a
2 ccsbBroad304_15839 pLX_304 0% 74.7% 64.1% V5 (many diffs) n/a
3 TRCN0000469244 GTTCACACACCAAGACTTGCGCAA pLX_317 18.2% 74.7% 64.1% V5 (many diffs) n/a
4 ccsbBroadEn_03305 pDONR223 100% 74.7% 64.1% None (many diffs) n/a
5 ccsbBroad304_03305 pLX_304 0% 74.7% 64.1% V5 (many diffs) n/a
6 TRCN0000470872 ATTGACGTCAGGCGACGCTCCCAT pLX_317 18.2% 74.7% 64.1% V5 (many diffs) n/a
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