Construct: ORF TRCN0000469253
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014822.1_s317c1
- Derived from:
- ccsbBroadEn_03721
- DNA Barcode:
- ACTTCCATTGTTTATTGGACCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CABP5 (56344)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469253
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 56344 | CABP5 | calcium binding protein 5 | NM_019855.5 | 99.8% | 100% | 238C>T |
2 | human | 56344 | CABP5 | calcium binding protein 5 | XM_017026992.1 | 60.5% | 60.2% | (many diffs) |
3 | mouse | 29865 | Cabp5 | calcium binding protein 5 | NM_013877.3 | 88.4% | 93% | (many diffs) |
4 | mouse | 29865 | Cabp5 | calcium binding protein 5 | XM_017322297.1 | 79.4% | 80.9% | (many diffs) |
5 | mouse | 29865 | Cabp5 | calcium binding protein 5 | XM_017322298.1 | 79.4% | 80.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 588
- ORF length:
- 519
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagttcccc atgggccccg cctgcatctt cttgaggaaa ggcattgctg 121 agaaacagcg ggaaagacca ctgggacaag atgagattga agagctgcgg gaagcatTTC 181 TTGAGTTCGA TAAGGACCGA GATGGGTTCA TCTCTTGTAA GGATCTGGGG AATCTCATGA 241 GGACGATGGG TTACATGCCC ACGGAGATGG AACTGATTGA GCTCGGCCAG CAAATCCGCA 301 TGAACTTGGG TGGCCGTGTA GACTTTGATG ACTTTGTGGA GCTGATGACC CCCAAATTGC 361 TTGCAGAAAC AGCTGGGATG ATCGGTGTCC AGGAGATGCG GGATGCCTTC AAGGAGTTTG 421 ACACGAATGG AGATGGGGAG ATCACCCTGG TGGAGCTACA GCAGGCCATG CAGAGACTCC 481 TGGGGGAGCG GCTCACCCCC CGGGAGATCT CTGAGGTTGT CCGGGAGGCT GATGTTAATG 541 GAGACGGCAC AGTTGACTTT GAAGAGTTTG TGAAGATGAT GTCTCGCTTG CCAACTTTCT 601 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 661 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 721 GTGGAAAGGA CGAACTTCCA TTGTTTATTG GACCATCACG CGTTAAGTCg acaatcaacc 781 tctggattac aaaatttgtg aaagatt