Construct: ORF TRCN0000469269
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009577.1_s317c1
- Derived from:
- ccsbBroadEn_03296
- DNA Barcode:
- AATGCGTGTTAACGGGATACGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF3L (51386)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469269
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51386 | EIF3L | eukaryotic translation init... | NM_016091.4 | 100% | 100% | |
| 2 | human | 51386 | EIF3L | eukaryotic translation init... | NM_001242923.2 | 91.4% | 91.4% | 435_436ins144 |
| 3 | human | 51386 | EIF3L | eukaryotic translation init... | XM_006724260.4 | 88.4% | 88.4% | 1_129del;1702_1703ins81 |
| 4 | human | 51386 | EIF3L | eukaryotic translation init... | NM_001363785.2 | 82.6% | 77.7% | 0_1ins214;78_79ins80 |
| 5 | human | 51386 | EIF3L | eukaryotic translation init... | XM_017028814.1 | 77.8% | 73% | 0_1ins214;78_79ins80;1279_1280ins81 |
| 6 | mouse | 223691 | Eif3l | eukaryotic translation init... | NM_145139.2 | 88.8% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1758
- ORF length:
- 1692
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ttatcccgct gatgattatg agtctgaggc ggcttatgac ccctacgctt 121 atcccagcga ctatgatatg cacacaggag atccaaagca ggaccttgct tatgaacgtc 181 agtatgaaca gcaaacctat caggtgatcc ctgaggtgat caaaaacttc atccagtatt 241 tccacaaaac tgtctcagat ttgattgacc agaaagtgta tgagctacag gccagtcgtg 301 tctccagtga tgtcattgac cagaaggtgt atgagatcca ggacatctat gagaacagct 361 ggaccaagct gactgaaaga ttcttcaaga atacaccttg gcccgaggct gaagccattg 421 ctccacaggt tggcaatgat gctgtcttcc tgattttata caaagaatta tactacaggc 481 acatatatgc caaagtcagt gggggacctt ccttggagca gaggtttgaa tcctattaca 541 actactgcaa tctcttcaac tacattctta atgccgatgg tcctgctccc cttgaactac 601 ccaaccagtg gctctgggat attatcgatg agttcatcta ccagtttcag tcattcagtc 661 agtaccgctg taagactgcc aagaagtcag aggaggagat tgactttctt cgttccaatc 721 ccaaaatctg gaatgttcat agtgtcctca atgtccttca ttccctggta gacaaatcca 781 acatcaaccg acagttggag gtatacacaa gcggaggtga ccctgagagt gtggctgggg 841 agtatgggcg gcactccctc tacaaaatgc ttggttactt cagcctggtc gggcttctcc 901 gcctgcactc cctgttagga gattactacc aggccatcaa ggtgctggag aacatcgaac 961 tgaacaagaa gagtatgtat tcccgtgtgc cagagtgcca ggtcaccaca tactattatg 1021 ttgggtttgc atatttgatg atgcgtcgtt accaggatgc catccgggtc ttcgccaaca 1081 tcctcctcta catccagagg accaagagca tgttccagag gaccacgtac aagtatgaga 1141 tgattaacaa gcagaatgag cagatgcatg cgctgctggc cattgccctc acgatgtacc 1201 ccatgcgtat tgatgagagc attcacctcc agctgcggga gaaatatggg gacaagatgt 1261 tgcgcatgca gaaaggtgac ccacaagtct atgaagaact tttcagttac tcctgcccca 1321 agttcctgtc gcctgtagtg cccaactatg ataatgtgca ccccaactac cacaaagagc 1381 ccttcctgca gcagctgaag gtgttttcTG ATGAAGTACA GCAGCAGGCC CAGCTTTCAA 1441 CCATCCGCAG CTTCCTGAAG CTCTACACCA CCATGCCTGT GGCCAAGCTG GCTGGCTTCC 1501 TGGACCTCAC AGAGCAGGAG TTCCGGATCC AGCTTCTTGT CTTCAAACAC AAGATGAAGA 1561 ACCTCGTGTG GACCAGCGGT ATCTCAGCCC TGGATGGTGA ATTTCAGTCA GCCTCAGAGG 1621 TTGACTTCTA CATTGATAAG GACATGATCC ACATCGCGGA CACCAAGGTC GCCAGGCGTT 1681 ATGGGGATTT CTTCATCCGT CAGATCCACA AATTTGAGGA GCTTAATCGA ACCCTGAAGA 1741 AGATGGGACA GAGACCTTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1801 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1861 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAATGCGT GTTAACGGGA 1921 TACGATTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt