Transcript: Human NM_001363785.2

Homo sapiens eukaryotic translation initiation factor 3 subunit L (EIF3L), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
EIF3L (51386)
Length:
2589
CDS:
246..1646

Additional Resources:

NCBI RefSeq record:
NM_001363785.2
NBCI Gene record:
EIF3L (51386)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001363785.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000074844 GCAGAGGTTTGAATCCTATTA pLKO.1 404 CDS 100% 13.200 10.560 N EIF3L n/a
2 TRCN0000307934 GCAGAGGTTTGAATCCTATTA pLKO_005 404 CDS 100% 13.200 10.560 N EIF3L n/a
3 TRCN0000074846 CCTGGTAGACAAATCCAACAT pLKO.1 650 CDS 100% 4.950 3.465 N EIF3L n/a
4 TRCN0000307937 CCTGGTAGACAAATCCAACAT pLKO_005 650 CDS 100% 4.950 3.465 N EIF3L n/a
5 TRCN0000074845 CCTTGCTTATGAACGTCAGTA pLKO.1 130 5UTR 100% 4.950 3.465 N EIF3L n/a
6 TRCN0000292011 CCTTGCTTATGAACGTCAGTA pLKO_005 130 5UTR 100% 4.950 3.465 N EIF3L n/a
7 TRCN0000074847 CTGGGATATTATCGATGAGTT pLKO.1 500 CDS 100% 4.950 3.465 N EIF3L n/a
8 TRCN0000292010 CTGGGATATTATCGATGAGTT pLKO_005 500 CDS 100% 4.950 3.465 N EIF3L n/a
9 TRCN0000074843 ACACACATTCAGGAACCTGTT pLKO.1 1655 3UTR 100% 4.050 2.835 N EIF3L n/a
10 TRCN0000292009 ACACACATTCAGGAACCTGTT pLKO_005 1655 3UTR 100% 4.050 2.835 N EIF3L n/a
11 TRCN0000138395 CCTTCCAAGTAGCTGGGATTA pLKO.1 2041 3UTR 100% 10.800 5.400 Y C11orf88 n/a
12 TRCN0000264189 CAAGTAGCTGGGACTACAGGA pLKO_005 2356 3UTR 100% 2.640 1.320 Y LINC01098 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001363785.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03296 pDONR223 100% 82.6% 77.7% None 0_1ins214;78_79ins80 n/a
2 ccsbBroad304_03296 pLX_304 0% 82.6% 77.7% V5 0_1ins214;78_79ins80 n/a
3 TRCN0000469269 AATGCGTGTTAACGGGATACGATT pLX_317 21% 82.6% 77.7% V5 0_1ins214;78_79ins80 n/a
4 ccsbBroadEn_15838 pDONR223 0% 82.5% 77.7% None 0_1ins214;78_79ins80;852T>C n/a
5 ccsbBroad304_15838 pLX_304 0% 82.5% 77.7% V5 0_1ins214;78_79ins80;852T>C n/a
6 TRCN0000477125 ATTACAACTCCCTTTCCAAACAGC pLX_317 21.7% 82.5% 77.7% V5 0_1ins214;78_79ins80;852T>C n/a
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