Construct: ORF TRCN0000469270
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017480.1_s317c1
- Derived from:
- ccsbBroadEn_02983
- DNA Barcode:
- TTACCGGAATGATGAGATGCCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPTN (27020)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469270
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27020 | NPTN | neuroplastin | NM_017455.4 | 100% | 100% | |
| 2 | human | 27020 | NPTN | neuroplastin | NM_001161364.2 | 98.5% | 98.5% | 765_766insGATGATGAACCA |
| 3 | human | 27020 | NPTN | neuroplastin | XM_024449899.1 | 76% | 71.7% | (many diffs) |
| 4 | human | 27020 | NPTN | neuroplastin | XM_024449900.1 | 74.9% | 70.6% | (many diffs) |
| 5 | human | 27020 | NPTN | neuroplastin | NM_012428.4 | 70.8% | 70.6% | 92_439del |
| 6 | human | 27020 | NPTN | neuroplastin | NM_001161363.2 | 69.8% | 69.5% | 92_439del;1113_1114insGATGATGAACCA |
| 7 | human | 27020 | NPTN | neuroplastin | XM_011521452.3 | 64.2% | 64% | 92_439del;1116_1116delCins79 |
| 8 | human | 27020 | NPTN | neuroplastin | XR_001751222.2 | 41.9% | (many diffs) | |
| 9 | human | 27020 | NPTN | neuroplastin | XR_001751221.2 | 41.8% | 1_32del;798_890del;972_2023del | |
| 10 | human | 27020 | NPTN | neuroplastin | XR_001751223.2 | 39.6% | 1_32del;799_1003del;1084_2135del | |
| 11 | human | 27020 | NPTN | neuroplastin | XR_001751219.1 | 35.3% | (many diffs) | |
| 12 | human | 27020 | NPTN | neuroplastin | XR_001751217.1 | 35.2% | (many diffs) | |
| 13 | human | 27020 | NPTN | neuroplastin | XR_001751220.1 | 33.6% | (many diffs) | |
| 14 | human | 27020 | NPTN | neuroplastin | XR_001751216.1 | 32.4% | (many diffs) | |
| 15 | mouse | 20320 | Nptn | neuroplastin | NM_009145.2 | 91.1% | 93.2% | (many diffs) |
| 16 | mouse | 20320 | Nptn | neuroplastin | NM_001293673.1 | 89.7% | 91.8% | (many diffs) |
| 17 | mouse | 20320 | Nptn | neuroplastin | XM_006510882.1 | 64.7% | 65.8% | (many diffs) |
| 18 | mouse | 20320 | Nptn | neuroplastin | XM_006510883.1 | 64.7% | 65.8% | (many diffs) |
| 19 | mouse | 20320 | Nptn | neuroplastin | XM_006510884.1 | 63.7% | 64.8% | (many diffs) |
| 20 | mouse | 20320 | Nptn | neuroplastin | XM_017313224.1 | 63.7% | 64.8% | (many diffs) |
| 21 | mouse | 20320 | Nptn | neuroplastin | XM_006510885.2 | 59.4% | 58.2% | (many diffs) |
| 22 | mouse | 20320 | Nptn | neuroplastin | XM_006510886.2 | 58.8% | 59.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 915
- ORF length:
- 846
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcgggttcg tcgctgccca gcgccctggc cctctcgctg ttgctggtct 121 ctggctccct cctcccaggg ccaggcgccg ctcagaacga gccaaggatt gtcaccagtg 181 aagaggtcat tattcgagac agccctgttc tccctgtcac cctgcagtgt aacctcacct 241 ccagctctca cacccttaca tacagctact ggacaaagaa tggggtggaa ctgagtgcca 301 ctcgtaagaa tgccagcaac atggagtaca ggatcaataa gccgagagct gaggattcag 361 gcgaatacca ctgcgtatat cactttgtca gcgctcctaa agcaaacgcc accattgaag 421 tgaaagccgc tcctgacatc actggccata aacggagtga gaacaagaat gaagggcagg 481 atgccactat gtattgcaag tcagttGGCT ACCCCCACCC AGACTGGATA TGGCGCAAGA 541 AGGAGAACGG GATGCCCATG GACATTGTCA ATACCTCTGG CCGCTTCTTC ATCATCAACA 601 AGGAAAATTA CACTGAGTTG AACATTGTGA ACCTGCAGAT CACGGAAGAC CCTGGCGAGT 661 ATGAATGTAA TGCCACCAAC GCCATTGGCT CCGCCTCTGT TGTCACTGTC CTCAGGGTGC 721 GGAGCCACCT GGCCCCACTC TGGCCTTTCT TGGGAATTCT GGCTGAAATT ATCATCCTTG 781 TGGTGATCAT TGTTGTGTAT GAGAAGAGGA AGAGGCCAGA TGAGGTTCCT GACGATGATG 841 AACCAGCTGG ACCAATGAAA ACCAACTCTA CCAACAATCA CAAAGATAAA AACTTGCGCC 901 AGAGAAACAC AAATTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 961 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTGAAAGTAT 1021 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TACCGGAATG ATGAGATGCC 1081 CCTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att