Transcript: Mouse XM_006510883.1

PREDICTED: Mus musculus neuroplastin (Nptn), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Nptn (20320)
Length:
2438
CDS:
192..1385

Additional Resources:

NCBI RefSeq record:
XM_006510883.1
NBCI Gene record:
Nptn (20320)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006510883.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000247867 TCTAAGGGCTTACCGTATAAC pLKO_005 1985 3UTR 100% 13.200 18.480 N Nptn n/a
2 TRCN0000247863 CACCAGTGAAGAGGTCATTAT pLKO_005 644 CDS 100% 13.200 10.560 N Nptn n/a
3 TRCN0000296825 CACCAGTGAAGAGGTCATTAT pLKO_005 644 CDS 100% 13.200 10.560 N NPTN n/a
4 TRCN0000061120 GTCGCCCATGTCAGAAACTAA pLKO.1 296 CDS 100% 5.625 4.500 N NPTN n/a
5 TRCN0000344926 ACCAGTGAAGAGGTCATTATT pLKO_005 645 CDS 100% 15.000 10.500 N NPTN n/a
6 TRCN0000061121 CATGGAGTACAGGATCAATAA pLKO.1 788 CDS 100% 13.200 9.240 N NPTN n/a
7 TRCN0000193073 CATGGAGTACAGGATCAATAA pLKO.1 788 CDS 100% 13.200 9.240 N Nptn n/a
8 TRCN0000247865 CATGGAGTACAGGATCAATAA pLKO_005 788 CDS 100% 13.200 9.240 N Nptn n/a
9 TRCN0000291393 CATGGAGTACAGGATCAATAA pLKO_005 788 CDS 100% 13.200 9.240 N NPTN n/a
10 TRCN0000061118 GCGTGAGTGTGCTGAGAATAA pLKO.1 481 CDS 100% 13.200 9.240 N NPTN n/a
11 TRCN0000247864 ATTACACTGAATTGAGTATTG pLKO_005 1075 CDS 100% 10.800 7.560 N Nptn n/a
12 TRCN0000247866 TCATCCTTGTGGTGATCATTG pLKO_005 1240 CDS 100% 10.800 7.560 N Nptn n/a
13 TRCN0000061122 CTACCAACAATCACAAAGATA pLKO.1 1336 CDS 100% 5.625 3.938 N NPTN n/a
14 TRCN0000193651 CCAAGAATTGTCACCAGTGAA pLKO.1 633 CDS 100% 4.950 3.465 N Nptn n/a
15 TRCN0000174908 GTATGAATGTAATGCCACCAA pLKO.1 1127 CDS 100% 2.640 1.848 N Nptn n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006510883.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02983 pDONR223 100% 64.7% 65.8% None (many diffs) n/a
2 ccsbBroad304_02983 pLX_304 0% 64.7% 65.8% V5 (many diffs) n/a
3 TRCN0000469270 TTACCGGAATGATGAGATGCCCCT pLX_317 48.2% 64.7% 65.8% V5 (many diffs) n/a
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