Construct: ORF TRCN0000469272
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002243.1_s317c1
- Derived from:
- ccsbBroadEn_00475
- DNA Barcode:
- GGAGTCGTGAAACAACACGTTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EDA (1896)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469272
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1896 | EDA | ectodysplasin A | NM_001399.5 | 100% | 100% | |
| 2 | human | 1896 | EDA | ectodysplasin A | NM_001005609.2 | 99.4% | 99.4% | 918_919insGTAGAA |
| 3 | human | 1896 | EDA | ectodysplasin A | XM_006724630.2 | 99.2% | 99.2% | 792_793insGATCTTTCA |
| 4 | human | 1896 | EDA | ectodysplasin A | NM_001005612.3 | 98.7% | 98.7% | 792_793insGATCTTTCA;909_910insGTAGAA |
| 5 | human | 1896 | EDA | ectodysplasin A | XM_017029336.1 | 96.4% | 96.4% | 882_883ins42 |
| 6 | human | 1896 | EDA | ectodysplasin A | XM_011530885.2 | 82.7% | 80.1% | (many diffs) |
| 7 | human | 1896 | EDA | ectodysplasin A | NM_001005613.4 | 35.9% | 33.5% | (many diffs) |
| 8 | human | 1896 | EDA | ectodysplasin A | NM_001005610.4 | 34.1% | 33.7% | (many diffs) |
| 9 | human | 1896 | EDA | ectodysplasin A | XM_017029337.1 | 34% | 33.7% | 397_398insTG;399_400ins772 |
| 10 | mouse | 13607 | Eda | ectodysplasin-A | NM_010099.2 | 91.4% | 94.6% | (many diffs) |
| 11 | mouse | 13607 | Eda | ectodysplasin-A | NM_001177937.1 | 90.9% | 94.1% | (many diffs) |
| 12 | mouse | 13607 | Eda | ectodysplasin-A | NM_001177938.1 | 90.7% | 93.8% | (many diffs) |
| 13 | mouse | 13607 | Eda | ectodysplasin-A | NM_001177939.1 | 90.1% | 93.3% | (many diffs) |
| 14 | mouse | 13607 | Eda | ectodysplasin-A | NM_001177940.1 | 89.5% | 92.5% | (many diffs) |
| 15 | mouse | 13607 | Eda | ectodysplasin-A | NM_001177941.1 | 88.3% | 91.3% | (many diffs) |
| 16 | mouse | 13607 | Eda | ectodysplasin-A | NM_001177942.1 | 87.8% | 91% | (many diffs) |
| 17 | mouse | 13607 | Eda | ectodysplasin-A | NM_001177943.1 | 78% | 71.5% | (many diffs) |
| 18 | mouse | 13607 | Eda | ectodysplasin-A | NM_001177944.1 | 78% | 71.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1242
- ORF length:
- 1173
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggctacccg gaggtggagc gcagggaact cctgcctgca gcagcgccgc 121 gggagcgagg gagccagggc tgcgggtgtg gcggggcccc tgcccgggcg ggcgaaggga 181 acagctgcct gctcttcctg ggtttctttg gcctctcgct ggccctccac ctgctgacgt 241 tgtgctgcta cctagagttg cgctcggagt tgcggcggga acgtggagcc gagtcccgcc 301 ttggcggctc gggcacccct ggcacctctg gcaccctaag cagcctcggt ggcctcgacc 361 ctgacagccc catcaccagt caccttgggc agccgtcacc taagcagcag ccattggaac 421 cgggagaagc cgcactccac tctgactccc aggacgggca ccagatggcc ctattgaatt 481 tcttcttccc tgatgaaaag ccatactctg aagaagaaag taggcgtgtt cgccgcaata 541 aaagaagcaa aagcaatgaa ggagcagatg gcccagttaa aaacaagaaa aagggaaaga 601 aagcaggacc tcctggaccc aatggccctc caggaccccc aggacctcca ggaccccagg 661 gacccccagg aattccaggg attcctggaa ttccaggaac aactgttatg ggaccacctg 721 gtcctccagg tcctcctggt cctcaaggac cccctggcct ccagggacct tctggtgctg 781 ctgataaagc tggaactcga gaaaaccagc cagctgtggt gcatctacag ggccaagggt 841 cagcaattca agtcaagaat gaTCTTTCAG GTGGAGTGCT CAATGACTGG TCTCGCATCA 901 CTATGAACCC CAAGGTGTTT AAGCTACATC CCCGCAGCGG GGAGCTGGAG GTACTGGTGG 961 ACGGCACCTA CTTCATCTAT AGTCAGGTAG AAGTATACTA CATCAACTTC ACTGACTTTG 1021 CCAGCTATGA GGTGGTGGTG GATGAGAAGC CCTTCCTGCA GTGCACACGC AGCATCGAGA 1081 CGGGCAAGAC CAACTACAAC ACTTGCTATA CCGCAGGCGT CTGCCTCCTC AAGGCCCGGC 1141 AGAAGATCGC CGTCAAGATG GTGCACGCTG ACATCTCCAT CAACATGAGC AAGCACACCA 1201 CGTTCTTTGG GGCCATCAGG CTGGGTGAAG CCCCTGCATC CTTGCCAACT TTCTTGTACA 1261 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1321 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1381 AGGACGAGGA GTCGTGAAAC AACACGTTTG CACGCGTTAA GTCgacaatc aacctctgga 1441 ttacaaaatt tgtgaaagat t