Construct: ORF TRCN0000469273
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015415.1_s317c1
- Derived from:
- ccsbBroadEn_12662
- DNA Barcode:
- ATCGAAAAACCCCCCCGTTCATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM62 (80021)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469273
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80021 | TMEM62 | transmembrane protein 62 | XM_011522073.1 | 100% | 100% | |
| 2 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347019.1 | 99.3% | 99.3% | 1_9delATGAAAAAG |
| 3 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347020.1 | 99.3% | 99.3% | 1_9delATGAAAAAG |
| 4 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347021.1 | 99.3% | 99.3% | 1_9delATGAAAAAG |
| 5 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347023.1 | 99.3% | 99.3% | 1_9delATGAAAAAG |
| 6 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347034.1 | 91.9% | 91.9% | 756_757ins105 |
| 7 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347024.1 | 91.3% | 91.3% | 1_9delATGAAAAAG;765_766ins105 |
| 8 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347025.1 | 91.3% | 91.3% | 1_9delATGAAAAAG;765_766ins105 |
| 9 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347026.1 | 91.3% | 91.3% | 1_9delATGAAAAAG;765_766ins105 |
| 10 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347027.1 | 91.3% | 91.3% | 1_9delATGAAAAAG;765_766ins105 |
| 11 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347028.1 | 91.3% | 91.3% | 1_9delATGAAAAAG;765_766ins105 |
| 12 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347029.1 | 91.3% | 91.3% | 1_9delATGAAAAAG;765_766ins105 |
| 13 | human | 80021 | TMEM62 | transmembrane protein 62 | XM_024450071.1 | 90.7% | 85.7% | 1_46del;163_164ins79 |
| 14 | human | 80021 | TMEM62 | transmembrane protein 62 | XM_011522074.2 | 90.1% | 84.3% | 1_55del;172_173ins79 |
| 15 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347008.1 | 85.1% | 85.1% | 1_228del |
| 16 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347009.1 | 85.1% | 85.1% | 1_228del |
| 17 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347010.1 | 85.1% | 85.1% | 1_228del |
| 18 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347011.1 | 85.1% | 85.1% | 1_228del |
| 19 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347012.1 | 85.1% | 85.1% | 1_228del |
| 20 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347013.1 | 85.1% | 85.1% | 1_228del |
| 21 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347004.1 | 84.7% | 84.7% | 1_234del |
| 22 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347005.1 | 84.7% | 84.7% | 1_234del |
| 23 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347006.1 | 84.7% | 84.7% | 1_234del |
| 24 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347007.1 | 84.7% | 84.7% | 1_234del |
| 25 | human | 80021 | TMEM62 | transmembrane protein 62 | XM_011522071.1 | 84.7% | 84.7% | 1_234del |
| 26 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347030.1 | 82.9% | 77.7% | 1_46del;163_164ins79;723_724ins105 |
| 27 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347017.1 | 78.2% | 78.2% | 1_228del;984_985ins105 |
| 28 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347018.1 | 78.2% | 78.2% | 1_228del;984_985ins105 |
| 29 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347014.1 | 77.9% | 77.9% | 1_234del;990_991ins105 |
| 30 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347015.1 | 77.9% | 77.9% | 1_234del;990_991ins105 |
| 31 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347016.1 | 77.9% | 77.9% | 1_234del;990_991ins105 |
| 32 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347033.1 | 70.4% | 69.1% | 1_436del;553_554ins79 |
| 33 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_024956.4 | 67.6% | 67.6% | 1_624del |
| 34 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347031.1 | 64.3% | 62.8% | 1_436del;553_554ins79;1113_1114ins105 |
| 35 | human | 80021 | TMEM62 | transmembrane protein 62 | NM_001347032.1 | 62.2% | 62.2% | 1_624del;1380_1381ins105 |
| 36 | human | 80021 | TMEM62 | transmembrane protein 62 | NR_144541.1 | 43.1% | (many diffs) | |
| 37 | human | 80021 | TMEM62 | transmembrane protein 62 | NR_144544.1 | 41.1% | (many diffs) | |
| 38 | human | 80021 | TMEM62 | transmembrane protein 62 | NR_144542.1 | 40.4% | (many diffs) | |
| 39 | human | 80021 | TMEM62 | transmembrane protein 62 | NR_144543.1 | 40% | (many diffs) | |
| 40 | human | 80021 | TMEM62 | transmembrane protein 62 | XM_024450072.1 | 24.1% | 21.5% | (many diffs) |
| 41 | mouse | 96957 | Tmem62 | transmembrane protein 62 | NM_175285.3 | 58.1% | 56.2% | (many diffs) |
| 42 | mouse | 96957 | Tmem62 | transmembrane protein 62 | XR_374546.3 | 28.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1371
- ORF length:
- 1305
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagctctta ttactggcca aggaaagcag tcggagcaac catacaattt 121 ggtttggaca ctttacaaca tccactattc tttctccatc accaggaatc cggtcaataa 181 tgagttcggc tatagcttat ttgtgtggac atctccatac acttggtgga ctgatgcctg 241 ttttgcacac tcgtcacttc cagggcactt tggaacttga ggtgggagac tggaaggata 301 ataggaggta ccggattttt gcttttgatc acgacctctt tagctttgca gatttgatct 361 ttgggaagtg gcctgtggtt cttatcacca atcctaaatc actcctttat agttgtggtg 421 aacatgaacc actagaaaga cttcttcact caacacacat cagagtcttg gccttttcct 481 tatcctccat tacttctgtc acagttaaga ttgatggagt tcatttaggc caggctgttc 541 atgtgtctgg tcccattttc gtactgaagt ggaatcctag aaactacagt agtgggacac 601 ataacataga agtaatcgtc caggattctg ctggaagaag taagagtgtt caccacatat 661 tttctgttca agagaataat catctcagtt ttgatcccct ggcatcattt attctccgta 721 ctgatcacta catcatggcc cgggtccttt ttgtgctgat tgtgctgagc cagctcacca 781 ttctcattat ttttagatat cgaggatacc cagagcttaa agaaccttca gggtttataa 841 atctgacctc attttctctt catgtcttga gcaaaataaa catcttctac tattctgtgt 901 tgttgttgac cctgtataca gtgctgggtc catggttttt tggtgaaatc attgatggca 961 aatttggttg ctgcttttcc tttgggatat ttgttaatgg aCATTTCCTA CAAGGCAGCA 1021 TAACATTTAT AATTGGAATT CTCCAGCTGG CGTTTTTTAA CATCCCCTTG ATGGCTTACA 1081 TGTGTTGGAG CTTGCTGCAG CGGTGCTTTG GTCACAACTT CAGGTCTCAT CTCCATCAAA 1141 GAAAATACTT GAAAATTATG CCTGTTCACC TACTTATGCT ACTGCTGTAC ATCTGGCAGG 1201 TTTATTCCTG CTACTTTCTT TATGCAACAT ACGGCACCCT AGCTTTTTTA TTCTCCCCTT 1261 TGCGGACCTG GTTGACACTG CTGACACCTG TTCTCATTCG TTATGTGTGG ACACTGAACT 1321 CCACCAAGTT TGGAATCTTC ATGGTGCAGT TAAAAAGCCA CCTGAGCTCC TGCCCAACTT 1381 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1441 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1501 CTTGTGGAAA GGACGAATCG AAAAACCCCC CCGTTCATTC ACGCGTTAAG TCgacaatca 1561 acctctggat tacaaaattt gtgaaagatt