Construct: ORF TRCN0000469290
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010379.1_s317c1
- Derived from:
- ccsbBroadEn_14238
- DNA Barcode:
- CTCAAGAGATTGGCTGTCACCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABRACL (58527)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469290
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58527 | ABRACL | ABRA C-terminal like | NM_021243.3 | 98.7% | 98.7% | 212_214delATG |
| 2 | human | 58527 | ABRACL | ABRA C-terminal like | XM_017011151.1 | 98.7% | 98.7% | 212_214delATG |
| 3 | mouse | 73112 | Abracl | ABRA C-terminal like | NM_028440.1 | 87.2% | 91.3% | (many diffs) |
| 4 | mouse | 73112 | Abracl | ABRA C-terminal like | XM_011243210.2 | 87.2% | 91.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 217
- ORF end:
- 457
- ORF length:
- 240
- Sequence:
-
1 GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA GACTGAAGTT AGGCCAGCTT 61 GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC 121 TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGCTAGC 181 ATCGATTGAT CAACAAGTTT GTACAAAAAA GTTGGCATGA ATGTGGATCA CGAGGTTAAC 241 CTCTTAGTGG AGGAAATTCA TCGTTTGGGT TCAAAAAATG CTGATGGAAA GTTAAGCGTG 301 AAATTTGGGG TCCTCTTCCG TGATGATAAA TGTGCCAACC TCTTTGAAGC ATTGGTAGGA 361 ACTCTTAAAG CTGCAAAACG AAGGAAGATT GTAACATATC CAGGAGAGCT GCTTCTGCAA 421 GGTGTTCATG ATGTTGACAT TATATTACTG CAAGATTACC CAACTTCCTT GTACAAAGTG 481 GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA 541 ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC 601 GACTCAAGAG ATTGGCTGTC ACCGCAACGC GTTAAGTCga caatcaacct ctggattaca 661 aaatttgtga aagatt