Construct: ORF TRCN0000469305
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016262.1_s317c1
- Derived from:
- ccsbBroadEn_08958
- DNA Barcode:
- GGGGCGAGCTCCCCTATAAGAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SUGCT (79783)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469305
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79783 | SUGCT | succinyl-CoA:glutarate-CoA ... | NM_001193311.1 | 99.8% | 99.7% | 678A>N;729A>G |
| 2 | human | 79783 | SUGCT | succinyl-CoA:glutarate-CoA ... | XM_006715775.3 | 95.9% | 94.3% | (many diffs) |
| 3 | human | 79783 | SUGCT | succinyl-CoA:glutarate-CoA ... | XM_011515525.3 | 94.6% | 94.5% | 678A>N;729A>G;1188_1265del |
| 4 | human | 79783 | SUGCT | succinyl-CoA:glutarate-CoA ... | NM_001193313.1 | 94.3% | 94.2% | 678A>N;729A>G;1109_1110ins78 |
| 5 | human | 79783 | SUGCT | succinyl-CoA:glutarate-CoA ... | NM_024728.2 | 92% | 91.9% | 372_373ins111;567A>N;618A>G |
| 6 | human | 79783 | SUGCT | succinyl-CoA:glutarate-CoA ... | XM_011515529.3 | 89.8% | 89.8% | 595_596ins144 |
| 7 | human | 79783 | SUGCT | succinyl-CoA:glutarate-CoA ... | XM_011515526.2 | 86.5% | 86.9% | (many diffs) |
| 8 | human | 79783 | SUGCT | succinyl-CoA:glutarate-CoA ... | XM_017012621.1 | 86.3% | 86.3% | (many diffs) |
| 9 | human | 79783 | SUGCT | succinyl-CoA:glutarate-CoA ... | XM_017012622.2 | 86.2% | 80.5% | (many diffs) |
| 10 | human | 79783 | SUGCT | succinyl-CoA:glutarate-CoA ... | XM_011515527.3 | 85.1% | 85.1% | 595_596ins144;1044_1121del |
| 11 | human | 79783 | SUGCT | succinyl-CoA:glutarate-CoA ... | NM_001193312.1 | 84.2% | 84.2% | 595_596ins144;965_966ins78 |
| 12 | human | 79783 | SUGCT | succinyl-CoA:glutarate-CoA ... | XM_011515528.3 | 83.3% | 76.3% | (many diffs) |
| 13 | human | 79783 | SUGCT | succinyl-CoA:glutarate-CoA ... | XM_011515530.3 | 62% | 59% | (many diffs) |
| 14 | mouse | 192136 | Sugct | succinyl-CoA glutarate-CoA ... | NM_138654.3 | 78.8% | 78% | (many diffs) |
| 15 | mouse | 192136 | Sugct | succinyl-CoA glutarate-CoA ... | XM_011244290.2 | 70.1% | 68.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1482
- ORF length:
- 1413
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccatctgag acgcacgcga tgctggcgac gctggcgagg gtggcagctc 121 tgcgcagaac ctgcctcttc tccggccggg gcggcgggag ggggctgtgg actggccgcc 181 cgcagtcaga tatgaacaat ataaagccat tggaaggggt aaaaattctg gatctaacaa 241 gagtcctggc gggacctttt gctactatga atttaggaga tcttggagca gaagttataa 301 aagtggagag accaggagct ggtgatgata cacgaacttg ggggccacct tttgttggga 361 cagaaagtac atattatctc agtgttaacc gaaataaaaa aagtattgct gttaatatca 421 aggatccaaa aggggtgaaa atcatcaaag agcttgcagc tgtttgtgat gtgtttgtgg 481 aaaactatgt ccctggcaaa ctgtctgcaa tgggcctggg atatgaagat atagacgaga 541 ttgctcctca catcatctat tgttccatca cagggtatgg tcagacaggt ccaatttctc 601 agcgagctgg ttatgatgct gttgcctcgg ctgtttctgg tctgatgcac atcacagggc 661 ctgagaatgg agatccagtt cgcccaggag tagctatgac tgatcttgcc actggcctgt 721 atgcatatgg agctattatg gctggnttga tacaaaaata caaaactggg aaaggactgt 781 tcattgattg taacctgctg tcatcccagg tggcgtgttt gtctcacata gctgcaaatt 841 atcttattgg tcaaaaggaa gcaaaacgtt ggggtacagc tcatggcagt atcgttcctt 901 accaggcttt taaaaccaag gatggctata ttgtagttgg agcaggaaat aaccagcagt 961 ttgccaccgt ctgcaagatc ttggatttgc ctgagttgat tgataattcc aagtataaaa 1021 ctaaccacct tcgggtacac aatagaaaag agcttattaa aatattaTCT GAACGGTTTG 1081 AAGAAGAACT GACCAGCAAG TGGTTATATC TTTTTGAAGG CAGTGGAGTC CCGTATGGCC 1141 CAATCAACAA CATGAAGAAT GTATTTGCAG AACCTCAGAA CGCTGTCTCT GGCTTCCAAA 1201 GCCTGCTGCA TTCCTTGGCC CATGGCCCCT TCCTTCATCT TCAAGGATCA GCAAGGGTAT 1261 TACACAATGG CCTCGTTATG GAGATGGAGC ATCCAACTGT GGGGAAGATT TCCGTCCCAG 1321 GCCCAGCTGT GAGATACAGT AAGTTCAAGA TGTCAGAGGC CAGGCCGCCC CCGCTGCTCG 1381 GGCAGCACAC AACGCACATC CTGAAGGAGG TCCTGAGATA CGATGACAGG GCCATCGGGG 1441 AGCTGCTCAG CGCTGGAGTG GTGGACCAAC ATGAAACTCA CTTGCCAACT TTCTTGTACA 1501 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1561 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1621 AGGACGAGGG GCGAGCTCCC CTATAAGAGT TACGCGTTAA GTCgacaatc aacctctgga 1681 ttacaaaatt tgtgaaagat t