Construct: ORF TRCN0000469355
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013673.1_s317c1
- Derived from:
- ccsbBroadEn_08750
- DNA Barcode:
- GGTCAGGACTTCCGGCACCAGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL1 (57626)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469355
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57626 | KLHL1 | kelch like family member 1 | NM_020866.3 | 99.8% | 99.8% | 1170A>G;1221A>G;1270C>G |
| 2 | human | 57626 | KLHL1 | kelch like family member 1 | NM_001286725.1 | 91.7% | 91.7% | (many diffs) |
| 3 | human | 57626 | KLHL1 | kelch like family member 1 | XM_017020678.2 | 76.7% | 76.7% | (many diffs) |
| 4 | human | 57626 | KLHL1 | kelch like family member 1 | XM_017020679.1 | 69.8% | 69.6% | (many diffs) |
| 5 | mouse | 93688 | Klhl1 | kelch-like 1 | NM_053105.2 | 89% | 95% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2310
- ORF length:
- 2244
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc aggctctggg cgaaaagact tcgatgtgaa gcacattctg cgactccgct 121 ggaaactctt cagccacccg tctccttcca ccggcggccc ggcgggggga ggctgcctgc 181 aacaggacgg cagtggcagc tttgagcact ggggacccag ccagagtcgc ctgctcaaaa 241 gccaagagag aagcggtgtg agcactttct ggaagaagcc ttcctcctct tcctcttcat 301 cgtcctcccc gtcctcttcc tcctcttcct tcaatccgct gaatggcacc ctgcttccag 361 ttgccacgag gctgcagcaa ggggctcctg ggcagggcac tcagcagcca gccaggactc 421 tcttctacgt ggagtcacta gaggaggagg tggtgccagg catggacttt cctggaccac 481 acgaaaaagg gctggttctg caagagctca aagtggagcc agacaactct agccaggcaa 541 caggtgaagg atgtggacac aggctgtcat caaccggcca ttcaatgaca cctcaaagtg 601 atttggactc cagtagctct gaagaattct atcaagctgt tcatcatgct gagcaaacct 661 tcagaaagat ggaaagttat ttgaagcagc agcaactttg tgatgttatc ctgattgttg 721 ggaaccgaaa gatacctgca cataggcttg ttctgagttc agtctccgac tattttgcgg 781 ccatgtttac aagtgatgtt tgtgaagcca agcaagagga gatcaaaatg gaaggcatag 841 accccaatgc tctctgggac cttgtccaat ttgcatatac aggctgcttg gaattaaaag 901 aggacaccat tgagaacctt cttgctgcag cgtgccttct tcagcttcca caggtggtgg 961 aagtgtgctg ccacttcctc atgaagcttt tgcatccatc taactgttta ggaattcgag 1021 ccttcgcaga tgctcaagga tgcattgagt taatgaaggt ggcccacagc tacacaatgg 1081 aaaacataat ggaagttatc agaaatcaag agtttttact ccttccagct gaggagctcc 1141 ataaactact ggccagtgat gatgtcaatg ttcctgatga agaaaccatc ttccatgcat 1201 tgatgatgtg ggtcaagtat gacatgcaga gtaggtgcaa tgacctgagc atgcttcttg 1261 cctttataag actgccactg cttccgccac agatattggc tgacctagaa aatcatgcat 1321 tatttaagaa tgatgtggaa tgtcaaaagc tgattctaga agcaatgaaa taccatctat 1381 tgccagaaag aagaacttta atgcaaagtc cgagaactaa acccagaaaa tctacagtcg 1441 gaactttgta tgctgtagga ggaatggata acaacaaagg agctacaact atagagaaat 1501 atgatctgag aacaaatctg tggatccagg cagggatgat gaatggcaga aggctgcagt 1561 ttggtgtggc tgttattgat gacaaactct ttgtaattgg aggtcgagat ggcttaaaga 1621 cattgaacac tgttgaatgt tacaatccca aaaccaagac atggactgtc ttaccaccaa 1681 tgtcaacaca cagacatggt ctaggtgtaa cagtacttga aggccctatt tatgctgtgg 1741 gaggccatga tggctggagc tatctgaata cagtggaaag gtgggatcca cagagtcaac 1801 aatggacatt tgtagccagt atgtcaattg ctcggagcac agttggtgta gcagcattga 1861 atggcaagtt gtattcagtt ggaggtcgtg atggaagttc ctgtttgagt tcaatggaat 1921 attatgatcc TCATACAAAT AAGTGGAACA TGTGTGCTCC CATGTGTAAG AGGAGAGGGG 1981 GTGTCGGAGT GGCCACATGT GACGGTTTTC TTTATGCAGT AGGAGGTCAT GATGCTCCTG 2041 CTTCAAATCA CTGTTCCCGG CTACTGGATT ATGTAGAAAG ATATGATCCC AAAACAGACA 2101 CTTGGACCAT GGTGGCTCCT TTGAGTATGC CCAGAGATGC TGTTGGGGTC TGTCTCCTTG 2161 GTGACAGATT ATATGCTGTT GGTGGCTATG ATGGACAGAC ATACCTCAAC ACTATGGAAT 2221 CCTATGACCC ACAAACTAAT GAGTGGACAC AGATGGCTTC CTTGAATATT GGGAGAGCAG 2281 GTGCCTGTGT GGTAGTCATC AAGCAACCTT GCCCAACTTT CTTGTACAAA GTGGTTGATA 2341 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2401 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGTCA 2461 GGACTTCCGG CACCAGCCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2521 tgaaagatt