Construct: ORF TRCN0000469355

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013673.1_s317c1
Derived from:
ccsbBroadEn_08750
DNA Barcode:
GGTCAGGACTTCCGGCACCAGCCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KLHL1 (57626)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469355
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57626 KLHL1 kelch like family member 1 NM_020866.3 99.8% 99.8% 1170A>G;1221A>G;1270C>G
2 human 57626 KLHL1 kelch like family member 1 NM_001286725.1 91.7% 91.7% (many diffs)
3 human 57626 KLHL1 kelch like family member 1 XM_017020678.2 76.7% 76.7% (many diffs)
4 human 57626 KLHL1 kelch like family member 1 XM_017020679.1 69.8% 69.6% (many diffs)
5 mouse 93688 Klhl1 kelch-like 1 NM_053105.2 89% 95% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2310
ORF length:
2244
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc aggctctggg cgaaaagact tcgatgtgaa gcacattctg cgactccgct
121ggaaactctt cagccacccg tctccttcca ccggcggccc ggcgggggga ggctgcctgc
181aacaggacgg cagtggcagc tttgagcact ggggacccag ccagagtcgc ctgctcaaaa
241gccaagagag aagcggtgtg agcactttct ggaagaagcc ttcctcctct tcctcttcat
301cgtcctcccc gtcctcttcc tcctcttcct tcaatccgct gaatggcacc ctgcttccag
361ttgccacgag gctgcagcaa ggggctcctg ggcagggcac tcagcagcca gccaggactc
421tcttctacgt ggagtcacta gaggaggagg tggtgccagg catggacttt cctggaccac
481acgaaaaagg gctggttctg caagagctca aagtggagcc agacaactct agccaggcaa
541caggtgaagg atgtggacac aggctgtcat caaccggcca ttcaatgaca cctcaaagtg
601atttggactc cagtagctct gaagaattct atcaagctgt tcatcatgct gagcaaacct
661tcagaaagat ggaaagttat ttgaagcagc agcaactttg tgatgttatc ctgattgttg
721ggaaccgaaa gatacctgca cataggcttg ttctgagttc agtctccgac tattttgcgg
781ccatgtttac aagtgatgtt tgtgaagcca agcaagagga gatcaaaatg gaaggcatag
841accccaatgc tctctgggac cttgtccaat ttgcatatac aggctgcttg gaattaaaag
901aggacaccat tgagaacctt cttgctgcag cgtgccttct tcagcttcca caggtggtgg
961aagtgtgctg ccacttcctc atgaagcttt tgcatccatc taactgttta ggaattcgag
1021ccttcgcaga tgctcaagga tgcattgagt taatgaaggt ggcccacagc tacacaatgg
1081aaaacataat ggaagttatc agaaatcaag agtttttact ccttccagct gaggagctcc
1141ataaactact ggccagtgat gatgtcaatg ttcctgatga agaaaccatc ttccatgcat
1201tgatgatgtg ggtcaagtat gacatgcaga gtaggtgcaa tgacctgagc atgcttcttg
1261cctttataag actgccactg cttccgccac agatattggc tgacctagaa aatcatgcat
1321tatttaagaa tgatgtggaa tgtcaaaagc tgattctaga agcaatgaaa taccatctat
1381tgccagaaag aagaacttta atgcaaagtc cgagaactaa acccagaaaa tctacagtcg
1441gaactttgta tgctgtagga ggaatggata acaacaaagg agctacaact atagagaaat
1501atgatctgag aacaaatctg tggatccagg cagggatgat gaatggcaga aggctgcagt
1561ttggtgtggc tgttattgat gacaaactct ttgtaattgg aggtcgagat ggcttaaaga
1621cattgaacac tgttgaatgt tacaatccca aaaccaagac atggactgtc ttaccaccaa
1681tgtcaacaca cagacatggt ctaggtgtaa cagtacttga aggccctatt tatgctgtgg
1741gaggccatga tggctggagc tatctgaata cagtggaaag gtgggatcca cagagtcaac
1801aatggacatt tgtagccagt atgtcaattg ctcggagcac agttggtgta gcagcattga
1861atggcaagtt gtattcagtt ggaggtcgtg atggaagttc ctgtttgagt tcaatggaat
1921attatgatcc TCATACAAAT AAGTGGAACA TGTGTGCTCC CATGTGTAAG AGGAGAGGGG
1981GTGTCGGAGT GGCCACATGT GACGGTTTTC TTTATGCAGT AGGAGGTCAT GATGCTCCTG
2041CTTCAAATCA CTGTTCCCGG CTACTGGATT ATGTAGAAAG ATATGATCCC AAAACAGACA
2101CTTGGACCAT GGTGGCTCCT TTGAGTATGC CCAGAGATGC TGTTGGGGTC TGTCTCCTTG
2161GTGACAGATT ATATGCTGTT GGTGGCTATG ATGGACAGAC ATACCTCAAC ACTATGGAAT
2221CCTATGACCC ACAAACTAAT GAGTGGACAC AGATGGCTTC CTTGAATATT GGGAGAGCAG
2281GTGCCTGTGT GGTAGTCATC AAGCAACCTT GCCCAACTTT CTTGTACAAA GTGGTTGATA
2341TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
2401CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGTCA
2461GGACTTCCGG CACCAGCCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
2521tgaaagatt

Download FASTA (ORF) (Full)